Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BBX44_RS16965 Genome accession   NZ_CP024024
Coordinates   3598918..3600618 (+) Length   566 a.a.
NCBI ID   WP_003112841.1    Uniprot ID   P22608
Organism   Pseudomonas aeruginosa strain PARM801     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3582013..3601842 3598918..3600618 within 0


Gene organization within MGE regions


Location: 3582013..3601842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBX44_RS16830 - 3582013..3582729 (-) 717 WP_073624880.1 hypothetical protein -
  BBX44_RS16835 - 3582878..3583078 (+) 201 WP_023435053.1 helix-turn-helix domain-containing protein -
  BBX44_RS16840 - 3583075..3583491 (+) 417 WP_023435054.1 hypothetical protein -
  BBX44_RS16845 - 3583484..3583717 (+) 234 WP_023110507.1 hypothetical protein -
  BBX44_RS16850 - 3583714..3583962 (+) 249 WP_073653397.1 hypothetical protein -
  BBX44_RS16855 - 3583959..3584264 (+) 306 WP_073653396.1 hypothetical protein -
  BBX44_RS33860 - 3584267..3584704 (+) 438 WP_343229373.1 hypothetical protein -
  BBX44_RS16865 - 3584743..3585210 (+) 468 WP_031629313.1 hypothetical protein -
  BBX44_RS16870 - 3585217..3586170 (+) 954 WP_023107347.1 DUF3631 domain-containing protein -
  BBX44_RS16875 - 3586170..3586463 (+) 294 WP_023107348.1 hypothetical protein -
  BBX44_RS16880 - 3586519..3587415 (+) 897 WP_023107349.1 hypothetical protein -
  BBX44_RS16885 - 3587546..3587959 (+) 414 WP_031629315.1 hypothetical protein -
  BBX44_RS16890 - 3588110..3588685 (-) 576 WP_124715997.1 hypothetical protein -
  BBX44_RS16895 - 3589422..3589928 (-) 507 WP_003096122.1 GNAT family N-acetyltransferase -
  BBX44_RS16900 - 3590689..3590970 (+) 282 WP_033978094.1 hypothetical protein -
  BBX44_RS16905 - 3590970..3591206 (+) 237 WP_003096116.1 hypothetical protein -
  BBX44_RS16910 - 3591413..3591694 (-) 282 WP_023435065.1 hypothetical protein -
  BBX44_RS16915 - 3591891..3592589 (+) 699 WP_124014670.1 hypothetical protein -
  BBX44_RS16920 - 3592614..3593618 (+) 1005 WP_031689993.1 pesticin C-terminus-like muramidase -
  BBX44_RS16925 - 3593631..3594293 (+) 663 WP_033992423.1 DUF3218 family protein -
  BBX44_RS16930 fliP 3594573..3595328 (+) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -
  BBX44_RS16935 fliQ 3595373..3595642 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  BBX44_RS16940 fliR 3595642..3596424 (+) 783 Protein_3366 flagellar biosynthetic protein FliR -
  BBX44_RS16945 - 3596427..3596686 (+) 260 Protein_3367 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  BBX44_RS16950 - 3597715..3597957 (+) 243 WP_023082027.1 hypothetical protein -
  BBX44_RS16960 pilA 3598237..3598686 (-) 450 WP_003112842.1 type 4a pilus biogenesis protein PilA Machinery gene
  BBX44_RS16965 pilB 3598918..3600618 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BBX44_RS16970 pilC 3600622..3601842 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62328.19 Da        Isoelectric Point: 5.7041

>NTDB_id=251239 BBX44_RS16965 WP_003112841.1 3598918..3600618(+) (pilB) [Pseudomonas aeruginosa strain PARM801]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQSKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=251239 BBX44_RS16965 WP_003112841.1 3598918..3600618(+) (pilB) [Pseudomonas aeruginosa strain PARM801]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAGCAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTTGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P22608

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.517

100

0.581
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.647

0.509
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.823

99.647

0.496
  pilF Thermus thermophilus HB27

39.75

99.117

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366

Multiple sequence alignment