Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EBN1_RS04935 Genome accession   NC_006513
Coordinates   1033788..1034921 (+) Length   377 a.a.
NCBI ID   WP_011236809.1    Uniprot ID   Q5P6H7
Organism   Aromatoleum aromaticum EbN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1028788..1039921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBN1_RS04910 (EB_RS04865) - 1030084..1030377 (-) 294 WP_011236804.1 DUF167 family protein -
  EBN1_RS04915 (EB_RS04870) - 1030383..1030961 (-) 579 WP_011236805.1 YggT family protein -
  EBN1_RS04920 (EB_RS04875) proC 1030968..1031780 (-) 813 WP_041646940.1 pyrroline-5-carboxylate reductase -
  EBN1_RS04925 (EB_RS04880) - 1031789..1032490 (-) 702 WP_011236807.1 YggS family pyridoxal phosphate-dependent enzyme -
  EBN1_RS04930 (EB_RS04885) pilT 1032594..1033637 (+) 1044 WP_011236808.1 type IV pilus twitching motility protein PilT Machinery gene
  EBN1_RS04935 (EB_RS04890) pilU 1033788..1034921 (+) 1134 WP_011236809.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EBN1_RS04940 (EB_RS04895) - 1034977..1036137 (-) 1161 WP_011236810.1 THUMP domain-containing protein -
  EBN1_RS04945 (EB_RS04900) - 1036243..1036662 (-) 420 WP_011236811.1 CopD family protein -
  EBN1_RS04950 (EB_RS04905) - 1036662..1037549 (-) 888 WP_011236812.1 SDR family oxidoreductase -
  EBN1_RS04955 (EB_RS04910) - 1037571..1038596 (+) 1026 WP_011236813.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  EBN1_RS04960 (EB_RS04915) - 1038601..1038774 (+) 174 Protein_967 regulator -
  EBN1_RS04965 (EB_RS04920) - 1038928..1039236 (-) 309 WP_041645820.1 c-type cytochrome -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42233.51 Da        Isoelectric Point: 6.4338

>NTDB_id=24107 EBN1_RS04935 WP_011236809.1 1033788..1034921(+) (pilU) [Aromatoleum aromaticum EbN1]
MERDQALKFMHDLLRLMVQKNGSDLFITAGFPPAIKVDGRVTPQSNQTLTAQHTTELARAIMNDRQAAEFESTKECNFAI
SPPGVGRFRANAFIQQGKVGLVLRTIAQTIPDFDELGMPAVLRDIAMAKRGLVIFVGGTGTGKTTSLAAMVDFRNEHSYG
HIITVEDPIEYVHQHKNCIVTQREIGIDTDNWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEDRRPQLLMDLSLNLRALISQRLLPLKERKGRVPAVEVLLNSPLIADLIFKGEVPGLKEVMKRSRELG
MQTFDQSLFDLYEEERISYEDALRNADSVNDLRLQIKLHSKHGDKDLVSGIQHLGIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=24107 EBN1_RS04935 WP_011236809.1 1033788..1034921(+) (pilU) [Aromatoleum aromaticum EbN1]
ATGGAACGCGATCAGGCCCTGAAGTTCATGCACGACCTGCTGCGGCTGATGGTGCAGAAAAATGGCTCGGACCTCTTCAT
CACCGCGGGCTTTCCGCCAGCAATCAAGGTGGACGGGCGCGTCACGCCGCAATCGAACCAGACGCTGACGGCGCAGCACA
CGACCGAGCTCGCGCGCGCGATCATGAACGACCGCCAGGCGGCCGAGTTCGAGTCGACGAAGGAATGCAACTTCGCGATC
TCGCCGCCGGGCGTCGGGCGCTTCCGCGCCAACGCGTTCATTCAGCAAGGCAAAGTGGGCCTCGTGCTGCGCACGATCGC
GCAGACGATCCCGGACTTCGACGAGCTCGGCATGCCGGCGGTGCTGCGCGACATCGCGATGGCCAAGCGTGGGCTGGTGA
TTTTCGTCGGCGGCACCGGCACCGGCAAGACGACGTCGCTCGCAGCGATGGTCGACTTCCGCAACGAACACTCGTACGGC
CACATCATCACTGTCGAGGATCCGATCGAATACGTGCATCAGCACAAGAACTGCATCGTCACGCAGCGCGAGATCGGCAT
CGACACCGACAACTGGGAAGCGGCGCTGAAGAACACGCTGCGCCAGGCACCCGACGTGATCCTGATGGGCGAAATCCGCG
ATCGCGAGACGATGGACTACGCGATCGCGTTCGCCGAAACCGGCCACCTGTGCCTCGCGACGCTGCATGCGAACAGCGCG
AACCAGGCGATCGACCGCATCATCAACTTCTTTCCCGAAGACCGCCGTCCGCAGCTCCTGATGGACCTGTCGCTGAACCT
GCGCGCGCTGATCTCGCAGCGGCTGCTGCCGCTCAAGGAGCGCAAGGGCCGCGTGCCGGCGGTCGAAGTGCTGCTGAATT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAAGTGCCGGGGCTCAAGGAAGTCATGAAGCGCTCGCGCGAGCTCGGC
ATGCAGACGTTCGACCAGAGCCTGTTCGACCTCTACGAGGAAGAGCGCATCAGCTACGAGGACGCGCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCCACAGCAAACACGGCGACAAGGACCTGGTGTCCGGCATCCAGCACC
TCGGGATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5P6H7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.155

96.021

0.597
  pilU Acinetobacter baylyi ADP1

57.939

95.225

0.552
  pilU Vibrio cholerae strain A1552

55

95.491

0.525
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.179

88.859

0.393
  pilT Acinetobacter baylyi ADP1

44.375

84.881

0.377
  pilT Pseudomonas stutzeri DSM 10701

42.388

88.859

0.377
  pilT Pseudomonas aeruginosa PAK

42.388

88.859

0.377
  pilT Acinetobacter nosocomialis M2

42.9

87.798

0.377
  pilT Acinetobacter baumannii D1279779

42.9

87.798

0.377
  pilT Acinetobacter baumannii strain A118

42.9

87.798

0.377
  pilT Legionella pneumophila strain Lp02

41.994

87.798

0.369
  pilT Legionella pneumophila strain ERS1305867

41.994

87.798

0.369

Multiple sequence alignment