Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CEW87_RS04700 Genome accession   NZ_CP022188
Coordinates   1007742..1008875 (+) Length   377 a.a.
NCBI ID   WP_108971667.1    Uniprot ID   -
Organism   Parazoarcus communis strain TSNA42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1002742..1013875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEW87_RS04675 (CEW87_04675) - 1004036..1004332 (-) 297 WP_108971663.1 DUF167 domain-containing protein -
  CEW87_RS04680 (CEW87_04680) - 1004329..1004910 (-) 582 WP_108971664.1 YggT family protein -
  CEW87_RS04685 (CEW87_04685) proC 1004917..1005741 (-) 825 WP_108971665.1 pyrroline-5-carboxylate reductase -
  CEW87_RS04690 (CEW87_04690) - 1005782..1006498 (-) 717 WP_108971666.1 YggS family pyridoxal phosphate-dependent enzyme -
  CEW87_RS04695 (CEW87_04695) pilT 1006601..1007644 (+) 1044 WP_108949787.1 type IV pilus twitching motility protein PilT Machinery gene
  CEW87_RS04700 (CEW87_04700) pilU 1007742..1008875 (+) 1134 WP_108971667.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CEW87_RS04705 (CEW87_04705) - 1008930..1010111 (-) 1182 WP_108971668.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  CEW87_RS04710 (CEW87_04710) - 1010366..1010581 (-) 216 WP_108971669.1 hypothetical protein -
  CEW87_RS04715 (CEW87_04715) - 1010578..1011612 (-) 1035 WP_108971670.1 formate dehydrogenase subunit gamma -
  CEW87_RS04720 (CEW87_04720) - 1011624..1011851 (-) 228 WP_108949791.1 hypothetical protein -
  CEW87_RS04725 (CEW87_04725) fdh3B 1011848..1012456 (-) 609 WP_108949792.1 formate dehydrogenase FDH3 subunit beta -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42206.61 Da        Isoelectric Point: 6.3150

>NTDB_id=237487 CEW87_RS04700 WP_108971667.1 1007742..1008875(+) (pilU) [Parazoarcus communis strain TSNA42]
MERDQALKFMHDLLRLMLQKNGSDLFINSGFPPAIKIDGRIVPQSNQALSPAHTAELARAIMNDRQAAEFEATKECNFAI
SPAGIGRFRANAYIQQGRVGLVLRTIALKIPTFDELGLPAVLRDIAMAKRGLVIFVGGTGTGKTTSLAAMVDYRNENSYG
HIITVEDPIEYVHQHKNCIISQREVGIDTENWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEERRSQLLMDLSLNLRSMISQRLMPLKDRKGRIPAVEVLLNSPLISDLIFKGEVPGIKEIMKRSRELG
MQTFDQSLFDLYEEGMITYEDALRNADSVNDLRLQIKLNSKLGDKDMSSGIQHLDIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=237487 CEW87_RS04700 WP_108971667.1 1007742..1008875(+) (pilU) [Parazoarcus communis strain TSNA42]
ATGGAACGCGATCAGGCACTCAAGTTCATGCATGACCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCCGACCTTTTCAT
CAACTCCGGATTTCCGCCGGCAATCAAGATCGACGGCCGCATCGTGCCGCAGTCGAACCAGGCGCTGAGCCCCGCTCACA
CCGCCGAACTCGCCCGCGCAATCATGAACGATCGCCAGGCCGCCGAGTTCGAGGCCACCAAGGAGTGCAACTTCGCGATC
TCGCCGGCCGGCATCGGCCGCTTCCGCGCCAATGCCTATATCCAGCAGGGGCGGGTCGGCCTCGTCCTGCGAACCATCGC
GCTGAAGATCCCAACTTTCGACGAACTGGGGCTGCCGGCCGTGCTGCGGGACATCGCAATGGCCAAACGTGGCCTGGTGA
TTTTCGTCGGCGGGACGGGTACCGGCAAGACGACCTCGCTGGCGGCGATGGTCGATTACCGCAACGAGAACAGCTACGGT
CACATCATCACCGTAGAAGACCCGATCGAGTATGTGCATCAGCACAAGAACTGCATCATTTCCCAGCGTGAAGTGGGCAT
CGACACCGAGAACTGGGAGGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACCATGGACTACGCGATCGCCTTCGCCGAAACCGGTCACCTGTGTCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCCATCGACCGCATCATCAACTTCTTCCCGGAAGAACGTCGCTCGCAGTTGCTGATGGACCTGTCGCTGAACCT
GCGCTCAATGATCTCGCAACGCCTGATGCCGCTCAAGGACCGCAAGGGCCGCATCCCGGCAGTCGAAGTCCTGCTCAATT
CGCCGCTGATCTCCGACCTCATCTTCAAGGGTGAGGTGCCGGGCATCAAGGAGATCATGAAGCGTTCGCGCGAGCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTACGAGGAAGGCATGATCACCTACGAGGATGCACTGCGAAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCAAGCTGGGCGACAAGGACATGAGCTCGGGCATCCAGCACC
TCGACATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.538

96.552

0.594
  pilU Acinetobacter baylyi ADP1

58.405

93.103

0.544
  pilU Vibrio cholerae strain A1552

55

95.491

0.525
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.296

94.96

0.411
  pilT Acinetobacter baylyi ADP1

42.433

89.39

0.379
  pilT Acinetobacter nosocomialis M2

42.598

87.798

0.374
  pilT Acinetobacter baumannii D1279779

42.598

87.798

0.374
  pilT Acinetobacter baumannii strain A118

42.598

87.798

0.374
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.859

0.371
  pilT Pseudomonas aeruginosa PAK

41.493

88.859

0.369
  pilT Legionella pneumophila strain Lp02

41.088

87.798

0.361
  pilT Legionella pneumophila strain ERS1305867

41.088

87.798

0.361

Multiple sequence alignment