Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CBP36_RS02130 Genome accession   NZ_CP021366
Coordinates   435095..436231 (-) Length   378 a.a.
NCBI ID   WP_086911109.1    Uniprot ID   A0A240TPE0
Organism   Acidovorax carolinensis strain P4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430095..441231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP36_RS02100 (CBP36_02095) - 430141..431076 (+) 936 WP_086926385.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  CBP36_RS02105 (CBP36_02100) rsmI 431197..432126 (-) 930 WP_204247598.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  CBP36_RS02110 (CBP36_02105) - 432126..432545 (+) 420 WP_086926386.1 YraN family protein -
  CBP36_RS02115 (CBP36_02110) - 432646..433245 (+) 600 WP_086926387.1 SIS domain-containing protein -
  CBP36_RS02120 (CBP36_02115) - 433242..433901 (+) 660 WP_086926388.1 BON domain-containing protein -
  CBP36_RS02125 (CBP36_02120) - 433963..434877 (-) 915 WP_086926389.1 NAD(P)-dependent oxidoreductase -
  CBP36_RS02130 (CBP36_02125) pilU 435095..436231 (-) 1137 WP_086911109.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CBP36_RS02135 (CBP36_02130) - 436275..436910 (-) 636 WP_086911110.1 cyclic nucleotide-binding domain-containing protein -
  CBP36_RS02140 (CBP36_02135) pilT 436981..438024 (-) 1044 WP_024813830.1 type IV pilus twitching motility protein PilT Machinery gene
  CBP36_RS02145 (CBP36_02140) - 438062..438778 (+) 717 WP_086926390.1 YggS family pyridoxal phosphate-dependent enzyme -
  CBP36_RS02150 (CBP36_02145) - 438931..440070 (+) 1140 WP_086926391.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41734.09 Da        Isoelectric Point: 6.9078

>NTDB_id=230202 CBP36_RS02130 WP_086911109.1 435095..436231(-) (pilU) [Acidovorax carolinensis strain P4]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTGTHTLTLARAVMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGKVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEVMKKSRNLG
MQTFDQALFDAYEANVISYEDALRNADSLNDLRLQIKLNSQRARSPDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=230202 CBP36_RS02130 WP_086911109.1 435095..436231(-) (pilU) [Acidovorax carolinensis strain P4]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGACTTGCTCAAACTGATGGTGAGCCGCAATGGCAGCGACTTGTTCAT
CACAGCCGAGTTTCCGCCGGCCATCAAGGTCGACGGAAAAGTGACCAAGGTATCGCCCCAGCCGCTGACGGGCACCCACA
CCCTCACGCTGGCGCGCGCCGTCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAATGCAACTTCGCCATC
TCGCCGGCCGGCATTGGCCGCTTTCGCGTGAACGCCTTCATCCAGCAGGGCAAGGTCGGCATGGTGTTGCGGACGATTCC
GCTCACGCTGCCCACCATTGACGGACTGGGCGTGCCGCAGGTTCTCAAGGAAGTGACGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAGTCCACCACGCTCGCGGCCATGGTGGACTGGCGCAACGAGAACTCGTTCGGC
CACATCATCACGGTGGAAGACCCGGTGGAATTCGTGCACCCGCACAAGAACTGCGTGGTGACGCAGCGTGAAGTGGGGCT
GGACACCGACAGCTGGGAAGCGGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAACATGCGGTGGCTTTCGCCGAAACCGGTCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGTTGCTGATGGATCTTTCGCTCAACCT
GCGCGCCATGGTGTCGCAGCGCCTTATCCCCAAACAGGATGGCAAGGGCCGGGCTGCGGCGGTGGAGGTCATGCTCAACA
CCCCGCTGATTGCCGACCTGATCTTCAAGGGCGAAGTCGCCGAGATCAAGGAGGTCATGAAAAAGAGCCGCAATCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATGCCTACGAGGCCAATGTGATCAGCTACGAAGACGCACTGCGCAACGCCGA
CTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCCAGGTCGCCCGATCTGGCCTCGGGCACCGAGC
ATTTCGCCATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A240TPE0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

58.263

94.444

0.55
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baylyi ADP1

43.26

84.392

0.365

Multiple sequence alignment