Detailed information NTG0022979

Bioinformatically predicted

Overview

• Name: pilA/pilAI
• Type: Machinery gene
• Locus tag: ALW85_RS06805
• Genome accession: NC_004757
• Coordinates: 1427752..1428162 (-)
• Length: 136 a.a.
• NCBI ID: WP_011111886.1
• Uniprot ID: Q820M2
• Organism: Nitrosomonas europaea ATCC 19718
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1422752..1433162

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ALW85_RS06765 (NE1300)	-	1423064..1423339 (-)	276	WP_011111878.1	hypothetical protein	-
ALW85_RS06770 (NE1301)	-	1423652..1424122 (+)	471	WP_011111879.1	methyltransferase family protein	-
ALW85_RS06775 (NE1302)	-	1424274..1425371 (-)	1098	WP_011111880.1	Rpn family recombination-promoting nuclease/putative transposase	-
ALW85_RS06780 (NE1303)	-	1425501..1425992 (+)	492	WP_011111881.1	FG-GAP repeat domain-containing protein	-
ALW85_RS06785 (NE1304)	-	1426056..1426358 (-)	303	WP_011111882.1	HigA family addiction module antitoxin	-
ALW85_RS06790 (NE1305)	-	1426371..1426649 (-)	279	WP_011111883.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ALW85_RS06795 (NE1306)	-	1426777..1427103 (-)	327	WP_011111884.1	type II toxin-antitoxin system PemK/MazF family toxin	-
ALW85_RS06800 (NE1307)	-	1427100..1427324 (-)	225	WP_041356736.1	antitoxin MazE family protein	-
ALW85_RS06805 (NE1308)	pilA/pilAI	1427752..1428162 (-)	411	WP_011111886.1	pilin	Machinery gene
ALW85_RS06810 (NE1310)	-	1429522..1430811 (-)	1290	WP_011111888.1	type II toxin-antitoxin system HipA family toxin	-
ALW85_RS06815 (NE1311)	-	1430801..1431061 (-)	261	WP_041356737.1	helix-turn-helix transcriptional regulator	-
ALW85_RS06825 (NE1312)	-	1431744..1431947 (-)	204	WP_011111890.1	transcription antiterminator/RNA stability regulator CspE	-
ALW85_RS14180	-	1432243..1432413 (+)	171	WP_158297464.1	hypothetical protein	-

Sequence

Protein

• Length: 136 a.a.
• Molecular weight: 14121.10 Da
• Isoelectric Point: 6.8976

>NTDB_id=22979 ALW85_RS06805 WP_011111886.1 1427752..1428162(-) (pilA/pilAI) [Nitrosomonas europaea ATCC 19718]
MQHTQKGFTLIELMIVVAIIGILAAVAIPAYSDYQAKSKVTAGLAEISAGKTAFEERVNNGDTVGTDPTVIGLKTPTSNC
TIVTSGTTIECTLTNAPSQVSGKKITLTRTDGTWSCATDADNKYAPKSCPGVTPTP

Nucleotide

• Length: 411 bp

>NTDB_id=22979 ALW85_RS06805 WP_011111886.1 1427752..1428162(-) (pilA/pilAI) [Nitrosomonas europaea ATCC 19718]
ATGCAACATACCCAAAAAGGTTTTACTTTGATTGAATTGATGATCGTCGTTGCGATCATCGGTATTTTGGCGGCCGTGGC
GATTCCGGCTTATTCAGATTATCAGGCTAAATCGAAAGTGACTGCTGGTCTTGCTGAAATTTCAGCAGGAAAGACCGCAT
TTGAGGAACGTGTCAATAACGGTGATACAGTTGGCACGGATCCTACGGTTATTGGTCTTAAAACACCGACTAGTAACTGT
ACTATTGTTACTTCTGGAACTACCATTGAATGTACGCTTACGAACGCTCCATCTCAAGTGAGTGGTAAAAAAATTACCTT
GACGCGTACTGATGGAACTTGGTCATGTGCTACTGATGCGGATAATAAATATGCTCCAAAGAGCTGTCCTGGCGTTACTC
CTACTCCTTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (36-129)

• Prokaryotic N-terminal methylation motif (N_methyl) (4-27)

3D structure

• AlphaFold DB: Q820M2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	51.111	99.265	0.507
pilA	Acinetobacter baumannii strain A118	46.853	100	0.493
pilA/pilAII	Pseudomonas stutzeri DSM 10701	47.727	97.059	0.463
comP	Acinetobacter baylyi ADP1	41.096	100	0.441
pilA	Pseudomonas aeruginosa PAK	41.379	100	0.441
pilA	Vibrio cholerae C6706	42.143	100	0.434
pilA	Vibrio cholerae strain A1552	42.143	100	0.434
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.143	100	0.434
pilA2	Legionella pneumophila strain ERS1305867	41.353	97.794	0.404
pilA2	Legionella pneumophila str. Paris	41.353	97.794	0.404
pilE	Neisseria gonorrhoeae strain FA1090	33.121	100	0.382
pilA	Vibrio parahaemolyticus RIMD 2210633	42.975	88.971	0.382