Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   B2G52_RS01500 Genome accession   NZ_CP019894
Coordinates   246014..246460 (+) Length   148 a.a.
NCBI ID   WP_003714658.1    Uniprot ID   A0A1V0DRU4
Organism   Neisseria lactamica strain Y92-1009     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 241014..251460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2G52_RS01465 (B2G52_01465) rpoN 241429..242772 (-) 1344 WP_003713045.1 RNA polymerase factor sigma-54 -
  B2G52_RS01470 (B2G52_01470) katA 243120..244634 (+) 1515 WP_003713046.1 catalase KatA -
  B2G52_RS12575 - 245111..245383 (+) 273 WP_231118393.1 hypothetical protein -
  B2G52_RS12805 - 245453..245581 (-) 129 WP_269202465.1 hypothetical protein -
  B2G52_RS12580 - 245586..245756 (+) 171 WP_231118394.1 hypothetical protein -
  B2G52_RS01500 (B2G52_01500) pilE 246014..246460 (+) 447 WP_003714658.1 pilin Machinery gene
  B2G52_RS01510 (B2G52_01510) - 247020..247586 (+) 567 WP_003714660.1 manganese efflux pump MntP family protein -
  B2G52_RS01520 (B2G52_01520) - 248012..250048 (-) 2037 WP_003714662.1 M3 family metallopeptidase -
  B2G52_RS01525 (B2G52_01525) - 250136..251008 (-) 873 WP_045750347.1 mechanosensitive ion channel family protein -
  B2G52_RS11555 - 251048..251275 (+) 228 WP_042508111.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15462.68 Da        Isoelectric Point: 8.9788

>NTDB_id=218713 B2G52_RS01500 WP_003714658.1 246014..246460(+) (pilE) [Neisseria lactamica strain Y92-1009]
MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEALTLAEGQKSAVVEYYSDNGTFPNTNASAGIANASDIK
GKYVASVNVSGTASLAKITATMNSSGVNNDIKGSTLVLEGKQNSGSFEWTCKAGTVKEKFLPSSCRAK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=218713 B2G52_RS01500 WP_003714658.1 246014..246460(+) (pilE) [Neisseria lactamica strain Y92-1009]
ATGAAAGCAATCCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTCATCGCCATCGTCGGTATCTTGGCAGCCGTCGC
CCTGCCCGCATACCAAGACTACACCGCGCGCGCCCAAATGTCCGAAGCCCTGACTTTGGCCGAAGGTCAAAAATCTGCAG
TGGTCGAATATTATTCCGACAACGGCACGTTCCCGAACACCAATGCTTCCGCAGGTATTGCTAATGCTAGCGATATTAAA
GGTAAGTATGTGGCATCGGTTAATGTTTCTGGCACTGCTTCCCTTGCTAAGATTACCGCTACCATGAACTCTTCCGGTGT
GAACAACGACATCAAAGGTTCAACCTTGGTATTGGAAGGCAAACAAAACTCCGGCTCGTTCGAATGGACTTGTAAAGCAG
GTACTGTGAAAGAAAAATTCCTGCCGTCTTCCTGCCGCGCCAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0DRU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

58.182

100

0.649
  pilE Neisseria gonorrhoeae strain FA1090

55.556

100

0.608
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.508

100

0.466
  pilA2 Legionella pneumophila str. Paris

45.07

95.946

0.432
  pilA/pilA1 Eikenella corrodens VA1

40.385

100

0.426
  pilA2 Legionella pneumophila strain ERS1305867

44.366

95.946

0.426
  pilA Ralstonia pseudosolanacearum GMI1000

37.126

100

0.419
  comP Acinetobacter baylyi ADP1

41.216

100

0.412
  pilA Pseudomonas aeruginosa PAK

37.255

100

0.385
  pilA Acinetobacter baumannii strain A118

37.086

100

0.378
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.356

98.649

0.378
  pilA Acinetobacter nosocomialis M2

45.528

83.108

0.378

Multiple sequence alignment