Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CWI20_RS25420 Genome accession   NZ_CP025056
Coordinates   5442594..5443058 (-) Length   154 a.a.
NCBI ID   WP_031630343.1    Uniprot ID   Q93PL5
Organism   Pseudomonas aeruginosa strain PB367     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5437594..5448058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI20_RS25395 (CWI20_25975) - 5438272..5438613 (-) 342 Protein_5042 HAMP domain-containing protein -
  CWI20_RS25400 - 5438608..5438850 (-) 243 Protein_5043 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CWI20_RS25405 (CWI20_25980) - 5439001..5441256 (-) 2256 WP_003094643.1 DUF1631 domain-containing protein -
  CWI20_RS25410 (CWI20_25985) nadC 5441542..5442390 (+) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CWI20_RS25420 (CWI20_25995) pilA 5442594..5443058 (-) 465 WP_031630343.1 pilin Machinery gene
  CWI20_RS25425 (CWI20_26000) pilB 5443288..5444988 (+) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CWI20_RS25430 (CWI20_26005) pilC 5444992..5446212 (+) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  CWI20_RS25435 (CWI20_26010) pilD 5446216..5447088 (+) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CWI20_RS25440 (CWI20_26015) coaE 5447085..5447696 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  CWI20_RS25445 (CWI20_26020) yacG 5447693..5447893 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15780.91 Da        Isoelectric Point: 6.1571

>NTDB_id=217211 CWI20_RS25420 WP_031630343.1 5442594..5443058(-) (pilA) [Pseudomonas aeruginosa strain PB367]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVSRSQVSRVMAEAGSLKTAVEACLQDGRTAVGTAAGQCDPGATGSSL
LTGASQTSQTLPTNTGVPQVLDPLTTQTTIIATFGNGASAAISGQTLTWTRDVNGGWSCATTVDAKFRPNGCTD

Nucleotide


Download         Length: 465 bp        

>NTDB_id=217211 CWI20_RS25420 WP_031630343.1 5442594..5443058(-) (pilA) [Pseudomonas aeruginosa strain PB367]
ATGAAAGCACAGAAGGGTTTTACTCTGATTGAACTGATGATCGTAGTTGCGATCATTGGTATTCTGGCTGCAATCGCAAT
TCCCCAATATCAGAACTATGTTTCTCGCTCTCAGGTCTCCAGGGTTATGGCAGAGGCTGGCTCCTTGAAGACTGCAGTTG
AGGCCTGCCTCCAGGATGGTCGTACTGCTGTGGGTACTGCTGCTGGTCAATGCGACCCGGGTGCGACGGGTTCCAGTTTG
TTGACTGGTGCTTCTCAGACTTCTCAAACCCTGCCAACCAATACCGGTGTTCCGCAGGTTCTGGATCCTCTGACTACTCA
AACCACTATCATTGCGACTTTTGGTAACGGTGCATCCGCAGCTATTTCTGGCCAGACTCTGACCTGGACTCGTGATGTTA
ATGGTGGCTGGAGCTGTGCTACTACCGTAGATGCTAAATTCCGTCCTAATGGCTGTACTGACTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q93PL5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

46.753

100

0.468
  pilA Vibrio cholerae strain A1552

43.59

100

0.442
  pilA Vibrio cholerae C6706

43.59

100

0.442
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.59

100

0.442
  comP Acinetobacter baylyi ADP1

40.12

100

0.435
  pilA Vibrio parahaemolyticus RIMD 2210633

40.881

100

0.422
  pilA Acinetobacter baumannii strain A118

41.83

99.351

0.416
  pilA2 Legionella pneumophila strain ERS1305867

41.558

100

0.416
  pilA2 Legionella pneumophila str. Paris

40.909

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40

100

0.403
  pilE Neisseria gonorrhoeae MS11

33.908

100

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.411

98.052

0.377