Detailed information NTG0215750

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PspS49_RS13425
• Genome accession: NZ_CP019432
• Coordinates: 3040026..3041135 (+)
• Length: 369 a.a.
• NCBI ID: WP_160039715.1
• Uniprot ID: A0A7Z2M7U6
• Organism: Pseudomonas sp. S49
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3035026..3046135

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PspS49_RS13410 (PspS49_13570)	-	3036014..3036955 (+)	942	WP_108227338.1	transposase	-
PspS49_RS13415 (PspS49_13575)	-	3037048..3037323 (-)	276	WP_007912322.1	peptidylprolyl isomerase	-
PspS49_RS13420 (PspS49_13580)	-	3037375..3039912 (-)	2538	WP_160039713.1	PAS domain S-box protein	-
PspS49_RS13425 (PspS49_13585)	pilU	3040026..3041135 (+)	1110	WP_160039715.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PspS49_RS13430 (PspS49_13590)	-	3041269..3045036 (+)	3768	WP_160039717.1	hypothetical protein	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40723.55 Da
• Isoelectric Point: 6.5984

>NTDB_id=215750 PspS49_RS13425 WP_160039715.1 3040026..3041135(+) (pilU) [Pseudomonas sp. S49]
MEIDALLSVLSNKNGSDLFLSTGAPPSARVDGVLTALSEQPFKIGETAAIAASLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFADLKLPPVLLDTVMFKQGLILFVGATDSGKSTSLAALIDHRNRHSTGHIITIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHATNANQALDRI
INMFPEERRPQLLQTLGNNLKAFISQRLVRTVDGQRRAAVEVLLGTPTIADLIRRGQFDELKPIMEKSPELGMQTFDAAL
FSLVAEGAISAQEALKNADSINNLKLRIKLAGDESSNNGSPSGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=215750 PspS49_RS13425 WP_160039715.1 3040026..3041135(+) (pilU) [Pseudomonas sp. S49]
ATGGAAATCGATGCACTGTTGTCAGTTTTGTCCAATAAAAACGGTTCGGATCTGTTCCTGTCCACGGGCGCACCGCCCAG
CGCGCGGGTCGACGGCGTGCTCACGGCATTGAGCGAGCAGCCTTTCAAGATCGGTGAAACCGCCGCCATCGCCGCCTCCC
TGATGGACGCCGAGCAACGACGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATTTCGCGAACGGGCATCGGCCGC
TTTCGCGTGAACATCTTCAAACAGCGCAACGATGTATCGATCGTTATCCGCAACGTCAAACTCGACATCCCGCGCTTCGC
TGACCTGAAACTGCCGCCGGTACTGCTCGACACCGTCATGTTCAAGCAAGGGCTGATCCTGTTTGTCGGCGCCACCGATT
CGGGTAAGTCGACTTCGCTGGCGGCATTGATCGACCACCGCAACCGCCACAGCACCGGGCACATCATCACCATCGAAGAT
CCGATCGAGTACATCCATCGGCACCAGCGTTCAATCATCAATCAACGTGAAGTCGGGGTCGATACGCGCAGTTTTCATGC
GGCGTTGAAGAACACTTTGCGCCAGGCGCCGGATGTAGTGCTGATCGGTGAAATCCGTGATCGCGAAACCATGGAACATG
CATTGGCGTTTGCCGATACCGGACATCTGGTGCTATCGACGTTGCATGCCACTAACGCCAATCAGGCGCTGGATCGAATT
ATCAATATGTTCCCGGAAGAGCGGCGACCGCAGTTATTGCAGACGCTGGGCAATAACCTGAAAGCATTTATCTCGCAACG
ATTGGTGCGCACTGTCGATGGCCAACGACGGGCTGCTGTCGAGGTGCTGTTGGGCACGCCAACCATCGCTGATCTGATCC
GACGCGGTCAGTTCGATGAACTCAAACCGATCATGGAGAAATCCCCCGAACTGGGTATGCAGACATTTGATGCAGCGTTG
TTTTCGTTGGTTGCCGAAGGTGCAATCAGTGCGCAGGAGGCTTTGAAAAATGCAGACTCGATCAATAATCTTAAATTACG
AATCAAACTGGCTGGAGATGAAAGTAGTAATAATGGTTCGCCGTCAGGGGAGTGGGGACTAATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (50-271)

3D structure

• AlphaFold DB: A0A7Z2M7U6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.435	99.729	0.553
pilU	Acinetobacter baylyi ADP1	54.108	95.664	0.518
pilU	Vibrio cholerae strain A1552	50.97	97.832	0.499
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.945	98.374	0.393
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.541	90.244	0.366
pilT	Vibrio cholerae strain A1552	40.541	90.244	0.366
pilT	Pseudomonas stutzeri DSM 10701	39.244	93.225	0.366
pilT	Acinetobacter baumannii D1279779	40	90.786	0.363
pilT	Acinetobacter baumannii strain A118	40	90.786	0.363
pilT	Pseudomonas aeruginosa PAK	38.953	93.225	0.363
pilT	Acinetobacter baylyi ADP1	39.118	92.141	0.36
pilT	Acinetobacter nosocomialis M2	39.701	90.786	0.36