Detailed information NTG0209720

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: BBX44_RS16960
• Genome accession: NZ_CP024024
• Coordinates: 3598237..3598686 (-)
• Length: 149 a.a.
• NCBI ID: WP_003112842.1
• Uniprot ID: P04739
• Organism: Pseudomonas aeruginosa strain PARM801
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3593237..3603686

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BBX44_RS16925	-	3593631..3594293 (+)	663	WP_033992423.1	DUF3218 family protein	-
BBX44_RS16930	fliP	3594573..3595328 (+)	756	WP_031285507.1	flagellar type III secretion system pore protein FliP	-
BBX44_RS16935	fliQ	3595373..3595642 (+)	270	WP_023435365.1	flagellar biosynthesis protein FliQ	-
BBX44_RS16940	fliR	3595642..3596424 (+)	783	Protein_3366	flagellar biosynthetic protein FliR	-
BBX44_RS16945	-	3596427..3596686 (+)	260	Protein_3367	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
BBX44_RS16950	-	3597715..3597957 (+)	243	WP_023082027.1	hypothetical protein	-
BBX44_RS16960	pilA	3598237..3598686 (-)	450	WP_003112842.1	type 4a pilus biogenesis protein PilA	Machinery gene
BBX44_RS16965	pilB	3598918..3600618 (+)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BBX44_RS16970	pilC	3600622..3601842 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
BBX44_RS16975	pilD	3601846..3602718 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
BBX44_RS16980	coaE	3602715..3603326 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
BBX44_RS16985	yacG	3603323..3603523 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 15511.78 Da
• Isoelectric Point: 6.2905

>NTDB_id=209720 BBX44_RS16960 WP_003112842.1 3598237..3598686(-) (pilA) [Pseudomonas aeruginosa strain PARM801]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV
GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide

• Length: 450 bp

>NTDB_id=209720 BBX44_RS16960 WP_003112842.1 3598237..3598686(-) (pilA) [Pseudomonas aeruginosa strain PARM801]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC
GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterProScan.

• Pilin (bacterial filament) (Pilin) (35-147)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

3D structure

• PDB: 1PAN
• PDB: 1PAO
• PDB: 8TUM
• PDB: 8TUW
• PDB: 9EWX

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	67.785	100	0.678
pilA	Acinetobacter baumannii strain A118	48	100	0.483
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	48.322	100	0.483
pilA	Vibrio cholerae strain A1552	48.322	100	0.483
pilA	Vibrio cholerae C6706	48.322	100	0.483
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.638	100	0.456
pilA	Vibrio parahaemolyticus RIMD 2210633	44.898	98.658	0.443
comP	Acinetobacter baylyi ADP1	41.83	100	0.43
pilA/pilAII	Pseudomonas stutzeri DSM 10701	44.056	95.973	0.423
pilA2	Legionella pneumophila str. Paris	38.776	98.658	0.383
pilA2	Legionella pneumophila strain ERS1305867	38.095	98.658	0.376