Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   BBX32_RS01020 Genome accession   NZ_CP018677
Coordinates   191465..191926 (-) Length   153 a.a.
NCBI ID   WP_000993711.1    Uniprot ID   A0A009I3S8
Organism   Acinetobacter baumannii strain LAC4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 186465..196926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBX32_RS00995 (BBX32_01005) - 186827..187210 (+) 384 WP_000090000.1 RidA family protein -
  BBX32_RS01000 (BBX32_01010) - 187420..187614 (+) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  BBX32_RS01005 (BBX32_01015) bfr 187858..188322 (+) 465 WP_000678123.1 bacterioferritin -
  BBX32_RS01010 (BBX32_01020) - 188363..189991 (-) 1629 WP_001195768.1 PglL family O-oligosaccharyltransferase -
  BBX32_RS01015 (BBX32_01025) - 190098..191408 (-) 1311 WP_000960539.1 O-antigen ligase family protein -
  BBX32_RS01020 (BBX32_01030) pilE 191465..191926 (-) 462 WP_000993711.1 pilin Machinery gene
  BBX32_RS01025 (BBX32_01035) - 192268..192828 (-) 561 WP_000258947.1 TPM domain-containing protein -
  BBX32_RS01030 (BBX32_01040) - 192822..193904 (-) 1083 WP_032003110.1 TPM domain-containing protein -
  BBX32_RS01035 (BBX32_01045) - 193926..194516 (-) 591 WP_000846931.1 LemA family protein -
  BBX32_RS01040 (BBX32_01050) - 194646..195533 (-) 888 WP_001090224.1 metal-dependent hydrolase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15590.71 Da        Isoelectric Point: 7.5868

>NTDB_id=209581 BBX32_RS01020 WP_000993711.1 191465..191926(-) (pilE) [Acinetobacter baumannii strain LAC4]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQDYIARSQVSEAMELASGLKGAVAETFSQDATCPTNGTNGIATATDINGK
YVASVTTAGTAAASGGCTITAKMRATGVSKGIQNGELTLTLSNADKGSNVWSCTSTGSNKIEQKYLPKSCTSS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=209581 BBX32_RS01020 WP_000993711.1 191465..191926(-) (pilE) [Acinetobacter baumannii strain LAC4]
ATGAATGCACAAAAAGGTTTTACGTTAATTGAACTCATGATCGTGGTTGCCATTATCGGTATCTTGGCAGCAATTGCGAT
TCCTGCGTATCAAGACTATATCGCTCGTTCTCAAGTTAGTGAAGCTATGGAGTTAGCTAGTGGATTGAAGGGGGCTGTTG
CAGAGACTTTTAGTCAAGATGCTACTTGCCCAACAAATGGTACTAATGGAATTGCTACTGCTACTGATATTAATGGTAAA
TATGTTGCTTCTGTAACCACGGCAGGTACCGCTGCTGCCTCAGGTGGATGTACAATTACTGCAAAAATGCGGGCAACTGG
AGTTTCAAAAGGTATTCAAAATGGAGAGCTTACTCTAACTCTTAGTAATGCAGATAAAGGTTCAAATGTGTGGAGTTGTA
CTTCAACTGGTTCAAACAAAATTGAACAAAAGTACTTACCTAAATCATGTACTTCTTCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009I3S8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

47.826

100

0.503
  pilE Neisseria gonorrhoeae MS11

43.21

100

0.458
  pilA2 Legionella pneumophila strain ERS1305867

42.581

100

0.431
  pilA Acinetobacter baumannii strain A118

41.509

100

0.431
  pilA2 Legionella pneumophila str. Paris

43.046

98.693

0.425
  pilA Acinetobacter nosocomialis M2

52.459

79.739

0.418
  comP Acinetobacter baylyi ADP1

42.384

98.693

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.447

99.346

0.412
  pilA Ralstonia pseudosolanacearum GMI1000

39.13

100

0.412
  pilA Pseudomonas aeruginosa PAK

39.744

100

0.405
  pilA/pilA1 Eikenella corrodens VA1

38.217

100

0.392
  pilA Vibrio parahaemolyticus RIMD 2210633

41.791

87.582

0.366

Multiple sequence alignment