Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BER93_RS15760 Genome accession   NZ_CP016833
Coordinates   3437823..3438956 (+) Length   377 a.a.
NCBI ID   WP_002814037.1    Uniprot ID   A0A1Y6GW32
Organism   Xanthomonas fragariae isolate Fap29     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3432823..3443956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BER93_RS15735 (BER93_15735) - 3433004..3434203 (-) 1200 WP_002814030.1 cystathionine gamma-synthase -
  BER93_RS15740 (BER93_15740) - 3434292..3435662 (-) 1371 WP_002814032.1 pyridoxal-phosphate dependent enzyme -
  BER93_RS15745 (BER93_15745) - 3435883..3436101 (-) 219 WP_002814034.1 YdcH family protein -
  BER93_RS15750 (BER93_15750) - 3436454..3437344 (-) 891 WP_002814035.1 hypothetical protein -
  BER93_RS15755 (BER93_15755) - 3437341..3437724 (-) 384 WP_002814036.1 DUF4398 domain-containing protein -
  BER93_RS15760 (BER93_15760) pilU 3437823..3438956 (+) 1134 WP_002814037.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BER93_RS15770 (BER93_15770) maiA 3439375..3440043 (-) 669 WP_002814039.1 maleylacetoacetate isomerase -
  BER93_RS15775 (BER93_15775) - 3440087..3441076 (-) 990 WP_002814041.1 fumarylacetoacetate hydrolase family protein -
  BER93_RS15780 (BER93_15780) - 3441454..3442848 (+) 1395 WP_002814042.1 DEAD/DEAH box helicase -
  BER93_RS15785 (BER93_15785) queD 3442965..3443318 (+) 354 WP_002814044.1 6-carboxytetrahydropterin synthase QueD -
  BER93_RS15790 (BER93_15790) - 3443524..3443900 (+) 377 Protein_3131 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42053.34 Da        Isoelectric Point: 6.2401

>NTDB_id=192558 BER93_RS15760 WP_002814037.1 3437823..3438956(+) (pilU) [Xanthomonas fragariae isolate Fap29]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGIVRKIAYSLMDEEQVRRFEHELEFNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDIIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTITGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFQESAHKNVLMNLALNLRAVVSQRLVKGADGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=192558 BER93_RS15760 WP_002814037.1 3437823..3438956(+) (pilU) [Xanthomonas fragariae isolate Fap29]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGATTGCCACCGGGAATAGTCAGGAAAATCGCATACTCGT
TGATGGACGAAGAACAAGTGCGGCGGTTCGAGCACGAGTTGGAATTCAACATGGCCATCGCCCTGCAAGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAACAGCGCGGCGAGGTGGGGATGGTGATCCGCGCCATCCGCAGCAGGATCCCAAGCATCGA
AGAACTCAATCTCCCGCAGGTGCTCAAGGACATCATCATGACGCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGTAAGTCGACCTCGCTGGCTTCGATGATCGACCACCGCAACAGCACCATTACCGGGCACATCCTCACTATCGAGGAC
CCGATCGAATATTTGCACAAGCACAAGATGTCGATCGTGAATCAGCGCGAGGTAGGTCTGGATACGCATACCTTCCAAAG
CGCGCTAAAGAACGCGATGCGTGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGTCTGGCGACGCTGCACTCCAACAATGCCGACCAGACTATCGAGCGCATC
CTCAATTTCTTCCAGGAAAGCGCGCACAAGAATGTGCTGATGAATCTGGCGCTGAATCTGCGCGCCGTCGTCTCTCAGCG
GCTGGTCAAGGGCGCGGACGGACGCCGCCGCCCCGCTACCGAAGTGCTGCTCAACACCCCGATGATTCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGGTCAAACAGGCGATGGAGGAATCTCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTCAAATCTGGCGAAATCGAGCAGGAGGAAGCACTGCGCGCAGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCTCCGGCGAGCACGACCCGTATGCGGATTACGAAACGGCAGCGGCGCCGAAGATCAGTC
ACGGGTTTATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y6GW32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

48.889

95.491

0.467
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.216

88.594

0.374
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.018

88.594

0.363

Multiple sequence alignment