Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BSY15_RS13415 Genome accession   NZ_CP016447
Coordinates   3032198..3033331 (-) Length   377 a.a.
NCBI ID   WP_069105230.1    Uniprot ID   A0A1B3PGW7
Organism   Acidovorax sp. RAC01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3027198..3038331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSY15_RS13390 (BSY15_2718) - 3027721..3028149 (+) 429 WP_069105226.1 Hsp20/alpha crystallin family protein -
  BSY15_RS13395 (BSY15_2719) - 3028198..3028869 (-) 672 WP_069105227.1 ABC transporter ATP-binding protein -
  BSY15_RS13400 (BSY15_2720) miaA 3028866..3029876 (-) 1011 WP_069106630.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  BSY15_RS13405 (BSY15_2721) - 3029863..3031104 (-) 1242 WP_069105228.1 multidrug effflux MFS transporter -
  BSY15_RS13410 (BSY15_2722) - 3031249..3032127 (-) 879 WP_069105229.1 alpha/beta fold hydrolase -
  BSY15_RS13415 (BSY15_2723) pilU 3032198..3033331 (-) 1134 WP_069105230.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BSY15_RS13420 (BSY15_2724) gdhA 3033461..3034804 (-) 1344 WP_069106631.1 NADP-specific glutamate dehydrogenase -
  BSY15_RS13425 (BSY15_2725) - 3035051..3036220 (+) 1170 WP_069105231.1 glycerophosphodiester phosphodiesterase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41468.73 Da        Isoelectric Point: 6.1874

>NTDB_id=188325 BSY15_RS13415 WP_069105230.1 3032198..3033331(-) (pilU) [Acidovorax sp. RAC01]
MEIFSFLNVMVEKGGSDLFFSVGAPVNLKIEGVTHPLKMPALLPGQVKQLAYAVMNEKQISEFEARQEMNLSISAENLGR
FRVNVFVQRGETGMVVRYIKNKIPPLAALGLPPILEKLVLHKRGLVLVTGATGSGKSTTLASMINYRNENVTGHILTIED
PLEFLHAHKRSVVDQREVGIDTHSYEEALKNALREAPDVIMIGEVRDRNTMKQAIAFAETGHLCLTTLHANNANQAMERV
INFFPEDAHHQLLMDLSLNLAGVVSQRLIPGLHEKLVPAVEVMLNSPYIADLIGKGEFASIKETMGHSTEIGMCTFDQAL
YQLYTEGRISLEEALHNADSRTDLALRVRLAAGVSAQDAGDLSIDTSSPMQSASRLY

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=188325 BSY15_RS13415 WP_069105230.1 3032198..3033331(-) (pilU) [Acidovorax sp. RAC01]
GTGGAGATCTTCAGCTTCCTCAATGTCATGGTCGAAAAAGGCGGCTCCGACCTGTTTTTCAGCGTGGGAGCCCCCGTCAA
CCTCAAGATCGAAGGCGTCACGCATCCGCTCAAGATGCCTGCGCTGCTGCCTGGCCAGGTCAAGCAGCTGGCCTATGCGG
TCATGAATGAAAAGCAGATCAGCGAGTTCGAGGCGCGGCAGGAGATGAACCTGTCGATCTCGGCCGAGAATCTGGGGCGC
TTTCGCGTCAACGTGTTCGTGCAGCGCGGCGAAACCGGCATGGTGGTGCGCTACATCAAGAACAAGATTCCGCCACTGGC
TGCACTGGGCCTGCCGCCCATCCTGGAAAAGCTGGTGCTGCACAAGCGCGGGCTGGTGCTGGTGACAGGCGCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCAACTACCGCAACGAAAACGTCACCGGTCACATCCTCACCATCGAAGAC
CCGCTGGAGTTCTTGCACGCGCACAAGCGCTCGGTGGTGGACCAGCGCGAGGTGGGCATCGACACGCACTCGTACGAAGA
AGCCCTGAAGAACGCGCTGCGCGAGGCGCCAGACGTGATCATGATCGGCGAGGTGCGCGACCGCAACACCATGAAGCAGG
CCATCGCGTTTGCCGAAACCGGGCACCTGTGCCTGACCACGCTGCACGCCAACAATGCCAACCAGGCCATGGAACGCGTT
ATCAACTTCTTCCCCGAAGACGCCCACCACCAGCTGCTGATGGACCTGAGCCTGAACCTGGCGGGCGTGGTGTCGCAGCG
GCTCATCCCGGGCCTGCATGAAAAGCTGGTGCCTGCGGTGGAAGTGATGCTCAACTCGCCCTACATCGCCGACCTGATCG
GCAAGGGCGAGTTTGCAAGCATCAAGGAAACCATGGGCCACAGCACCGAGATCGGCATGTGCACCTTCGACCAGGCGCTG
TACCAGCTGTACACCGAAGGTCGCATCTCGCTCGAAGAAGCCCTGCACAACGCCGACTCGCGCACCGACCTGGCGCTGCG
CGTGCGCCTGGCAGCGGGCGTGAGCGCGCAGGACGCGGGCGATCTCTCCATCGACACCTCCTCGCCCATGCAGTCGGCAT
CGCGCCTGTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B3PGW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.807

93.634

0.523
  pilU Vibrio cholerae strain A1552

51.695

93.899

0.485
  pilU Acinetobacter baylyi ADP1

50.708

93.634

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.896

94.695

0.387
  pilT Acinetobacter baumannii strain A118

41.916

88.594

0.371
  pilT Acinetobacter nosocomialis M2

41.916

88.594

0.371
  pilT Acinetobacter baumannii D1279779

41.916

88.594

0.371
  pilT Acinetobacter baylyi ADP1

41.617

88.594

0.369
  pilT Pseudomonas aeruginosa PAK

40.828

89.655

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.533

89.655

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.329

0.363
  pilT Vibrio cholerae strain A1552

41.141

88.329

0.363

Multiple sequence alignment