Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AMD27_RS01540 Genome accession   NZ_CP015110
Coordinates   339477..339917 (+) Length   146 a.a.
NCBI ID   WP_067655408.1    Uniprot ID   -
Organism   Acinetobacter sp. TGL-Y2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334477..344917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMD27_RS01515 (AMD27_01515) - 335521..335925 (+) 405 WP_067655393.1 ribosome-binding factor A -
  AMD27_RS01520 (AMD27_01520) - 336008..336829 (+) 822 WP_067655396.1 tetratricopeptide repeat protein -
  AMD27_RS01525 (AMD27_01525) - 336930..337520 (+) 591 WP_067655399.1 LemA family protein -
  AMD27_RS01530 (AMD27_01530) - 337609..338718 (+) 1110 WP_067655402.1 TPM domain-containing protein -
  AMD27_RS01535 (AMD27_01535) - 338712..339272 (+) 561 WP_067655405.1 TPM domain-containing protein -
  AMD27_RS01540 (AMD27_01540) pilA 339477..339917 (+) 441 WP_067655408.1 pilin Machinery gene
  AMD27_RS01545 (AMD27_01545) - 339951..341594 (+) 1644 WP_081405897.1 PglL family O-oligosaccharyltransferase -
  AMD27_RS01550 (AMD27_01550) - 341604..341957 (+) 354 WP_067655414.1 type IV pilin protein -
  AMD27_RS01555 (AMD27_01555) - 342153..343670 (+) 1518 WP_322843235.1 PglL family O-oligosaccharyltransferase -
  AMD27_RS01560 (AMD27_01560) bfr 343745..344209 (-) 465 WP_067655417.1 heteropolymeric bacterioferritin subunit Bfr -
  AMD27_RS01565 (AMD27_01565) - 344442..344636 (-) 195 WP_067655420.1 bacterioferritin-associated ferredoxin -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15071.36 Da        Isoelectric Point: 8.1097

>NTDB_id=176701 AMD27_RS01540 WP_067655408.1 339477..339917(+) (pilA) [Acinetobacter sp. TGL-Y2]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYTAKSQTAAGLAEISPAKTNAEAKLAEGISATIATASALGINDSSRCT
LKASVAADGASTIQCTLKGTKSVNGQIIQWVRTADNVDGTMGKWTCKTNVVEELRPKTCEGSPTAI

Nucleotide


Download         Length: 441 bp        

>NTDB_id=176701 AMD27_RS01540 WP_067655408.1 339477..339917(+) (pilA) [Acinetobacter sp. TGL-Y2]
ATGAACGCTCAAAAAGGTTTTACCTTAATTGAATTAATGATCGTTGTTGCGATTATCGGTATTTTGGCTGCGATTGCGAT
TCCTGCATACCAAAACTACACAGCTAAATCTCAAACTGCTGCTGGTCTTGCAGAAATTTCTCCAGCAAAAACAAATGCTG
AAGCGAAATTAGCTGAAGGAATTTCTGCAACAATTGCGACTGCTTCAGCGTTAGGTATTAATGATTCAAGTCGTTGCACT
CTCAAAGCCTCTGTTGCAGCAGATGGTGCTTCAACTATTCAATGCACTTTAAAAGGTACAAAATCTGTTAATGGACAGAT
TATCCAATGGGTTCGTACTGCTGATAATGTAGATGGTACAATGGGTAAATGGACTTGCAAAACTAATGTAGTTGAAGAGT
TACGTCCTAAAACTTGTGAAGGTTCACCGACTGCTATTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50

100

0.507
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.667

100

0.459
  pilA Vibrio cholerae strain A1552

44.667

100

0.459
  pilA Vibrio cholerae C6706

44.667

100

0.459
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.518

96.575

0.459
  pilA Pseudomonas aeruginosa PAK

42.675

100

0.459
  comP Acinetobacter baylyi ADP1

41.722

100

0.432
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.857

95.89

0.411
  pilA2 Legionella pneumophila str. Paris

42.857

95.89

0.411
  pilA2 Legionella pneumophila strain ERS1305867

42.857

95.89

0.411
  pilA/pilA1 Eikenella corrodens VA1

38.158

100

0.397
  pilA Vibrio parahaemolyticus RIMD 2210633

44.8

85.616

0.384

Multiple sequence alignment