Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A3K91_RS01710 Genome accession   NZ_CP014945
Coordinates   400155..401276 (-) Length   373 a.a.
NCBI ID   WP_062843733.1    Uniprot ID   -
Organism   Psychrobacter alimentarius strain PAMC 27889     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395155..406276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3K91_RS01680 (A3K91_0332) - 395742..396077 (+) 336 WP_062843729.1 c-type cytochrome -
  A3K91_RS01685 (A3K91_0333) - 396394..397062 (+) 669 WP_062843730.1 c-type cytochrome -
  A3K91_RS01690 (A3K91_0334) - 397298..398419 (+) 1122 WP_062843731.1 hypothetical protein -
  A3K91_RS01695 - 398407..398790 (+) 384 WP_228139887.1 RnfH family protein -
  A3K91_RS01700 (A3K91_0336) - 398839..399258 (-) 420 WP_062845808.1 outer membrane protein assembly factor BamE -
  A3K91_RS01705 (A3K91_0337) fur 399583..400017 (+) 435 WP_062843732.1 ferric iron uptake transcriptional regulator -
  A3K91_RS01710 (A3K91_0338) pilU 400155..401276 (-) 1122 WP_062843733.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A3K91_RS01715 (A3K91_0339) pilT 401424..402479 (-) 1056 WP_062843734.1 type IV pilus twitching motility protein PilT Machinery gene
  A3K91_RS01720 (A3K91_0340) - 402755..403477 (+) 723 WP_062843735.1 YggS family pyridoxal phosphate-dependent enzyme -
  A3K91_RS01725 (A3K91_0341) - 403648..404922 (-) 1275 WP_062843736.1 hemolysin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41699.97 Da        Isoelectric Point: 6.8289

>NTDB_id=175265 A3K91_RS01710 WP_062843733.1 400155..401276(-) (pilU) [Psychrobacter alimentarius strain PAMC 27889]
MDIDKLLQLMINKNGSDLFITADVAPSMKINGKILPVGKTPLSADQTMRLVKGVMTPSQQKEFEETNECQFAISDEAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSTTLAAMIGHRNHNSHGHIITIE
DPIEFVHKHRGCIITQREVGLDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRESGMQTFDQ
ALFDLYEQGEITYKEAILHADSPNDLRLKIKLSSKNGMANLDEGADSLSLDIT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=175265 A3K91_RS01710 WP_062843733.1 400155..401276(-) (pilU) [Psychrobacter alimentarius strain PAMC 27889]
ATGGATATTGATAAACTATTGCAGCTAATGATCAATAAAAATGGCTCTGATCTTTTTATTACCGCTGATGTGGCCCCTTC
CATGAAAATAAATGGAAAAATCCTGCCAGTAGGTAAAACACCGTTGAGTGCTGATCAAACCATGAGACTGGTCAAAGGGG
TAATGACGCCTTCTCAGCAAAAAGAATTTGAAGAGACCAATGAATGCCAGTTTGCTATCTCTGATGAGGCTCAACAAGCC
CGTTTTCGGGTTAGTGCTTTTATACAGCGTGACATGGCAGGAATGATTTTACGAAAAATTGAAAATAAAATCCCAACCGT
CGAAGAGCTACGCCTACCACCTGCTCTAAAAGAGCTGGCAATGAAAAAAAGAGGCATTATTTTATTGGTTGGTGCTACTG
GTACAGGCAAATCTACTACGCTTGCTGCCATGATCGGACATCGCAATCACAATTCTCATGGTCATATCATCACTATTGAA
GATCCGATTGAATTTGTCCATAAACATCGTGGCTGCATCATCACCCAACGTGAAGTTGGTCTCGATACCCACTCGTTTGA
AGCGGGTCTAAAAAATACTTTGCGCCAAGCGCCTGATGTTATTTTAATTGGTGAGATTCGCAACCGAGAGGTCATGAGCT
ATGCCATTCAATATGCCGAAACAGGGCATCTGGTATTTGCAACATTACACGCAAACAATGCCAACCAAGCCATTGACCGT
ATTATTCACTTTTTTGAAACCAGTCGTCACAATCAGCTATTTATGGATTTGTCCCTAAACCTACAAGCCATTATTGCTCA
GCAGCTCATTCCAACTCCAGATGGTAAAGGGCGACGCGCTGCTATTGAGATTTTATTAAACTCCCAGCTGATTAGTGACT
ATATTCGTAAAGGTGAGGTTCATGAAATCAAAGATGTCATGACGCGTTCGCGTGAGAGTGGCATGCAAACCTTTGATCAA
GCTTTGTTTGACTTATATGAGCAAGGAGAAATCACATACAAAGAAGCGATTCTTCATGCGGATTCTCCCAATGACTTACG
CTTAAAAATCAAACTAAGTAGTAAAAATGGTATGGCGAATCTGGACGAAGGCGCTGATAGTTTATCGTTAGACATCACTT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.952

100

0.66
  pilU Acinetobacter baylyi ADP1

67.042

95.174

0.638
  pilU Vibrio cholerae strain A1552

52.717

98.66

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.35

98.123

0.416
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.194

89.812

0.37
  pilT Vibrio cholerae strain A1552

41.194

89.812

0.37
  pilT Acinetobacter nosocomialis M2

40.233

91.957

0.37
  pilT Acinetobacter baumannii D1279779

40.233

91.957

0.37
  pilT Acinetobacter baumannii strain A118

40.233

91.957

0.37
  pilT Legionella pneumophila strain Lp02

41.071

90.08

0.37
  pilT Legionella pneumophila strain ERS1305867

41.071

90.08

0.37
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365

Multiple sequence alignment