Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AL536_RS30945 Genome accession   NZ_CP014035
Coordinates   109248..109667 (-) Length   139 a.a.
NCBI ID   WP_047459095.1    Uniprot ID   A0AAX2LK83
Organism   Vibrio fluvialis strain ATCC 33809     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 104248..114667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL536_RS30925 (AL536_08145) coaE 104756..105364 (-) 609 WP_061056140.1 dephospho-CoA kinase -
  AL536_RS30930 (AL536_08150) pilD 105367..106236 (-) 870 WP_024374403.1 prepilin peptidase Machinery gene
  AL536_RS30935 (AL536_08155) pilC 106288..107514 (-) 1227 WP_061056141.1 type II secretion system F family protein Machinery gene
  AL536_RS30940 (AL536_08160) pilB 107550..109238 (-) 1689 WP_061056142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL536_RS30945 (AL536_08165) pilA 109248..109667 (-) 420 WP_047459095.1 pilin Machinery gene
  AL536_RS30950 (AL536_08170) nadC 109898..110785 (-) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL536_RS30955 (AL536_08175) ampD 110938..111489 (+) 552 WP_061056144.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL536_RS30960 (AL536_08180) fldB 111494..112015 (-) 522 WP_020430452.1 flavodoxin FldB -
  AL536_RS30965 (AL536_08185) xerD 112156..113064 (+) 909 WP_047459088.1 site-specific tyrosine recombinase XerD -
  AL536_RS30970 (AL536_08190) dsbC 113081..113845 (+) 765 WP_061056145.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14270.22 Da        Isoelectric Point: 8.4642

>NTDB_id=167680 AL536_RS30945 WP_047459095.1 109248..109667(-) (pilA) [Vibrio fluvialis strain ATCC 33809]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVTKSELASGFATIKSVLTPAELYVQENGKISSGVSTITSLGV
SSAANSLGTLSISADNEITFSHTDGSAKGAKFVYTRTTGGWSCAYTGPAAVSAAAPKGC

Nucleotide


Download         Length: 420 bp        

>NTDB_id=167680 AL536_RS30945 WP_047459095.1 109248..109667(-) (pilA) [Vibrio fluvialis strain ATCC 33809]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGAGTTCT
GTCGGCGATTGCCGTACCCGCTTATAAGGATTACGTAACCAAGAGTGAGTTAGCTTCTGGCTTTGCCACAATTAAATCCG
TGCTAACACCAGCAGAGTTATATGTCCAAGAAAATGGAAAGATTAGTAGTGGAGTAAGTACCATCACATCGCTGGGTGTT
TCCTCTGCCGCTAATAGCTTAGGCACACTAAGTATCTCAGCAGACAATGAAATCACCTTTTCTCACACTGATGGTTCAGC
AAAGGGCGCGAAGTTCGTATACACAAGAACAACTGGTGGCTGGTCATGTGCGTACACCGGCCCAGCAGCCGTGAGCGCAG
CAGCACCGAAAGGTTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

50.746

96.403

0.489
  pilA Acinetobacter baumannii strain A118

41.83

100

0.46
  pilA Pseudomonas aeruginosa PAK

40.667

100

0.439
  pilA Vibrio cholerae C6706

38.312

100

0.424
  pilA Vibrio cholerae strain A1552

38.312

100

0.424
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.312

100

0.424
  pilA2 Legionella pneumophila str. Paris

37.857

100

0.381
  pilA2 Legionella pneumophila strain ERS1305867

37.143

100

0.374
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.31

92.806

0.374

Multiple sequence alignment