Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   AL536_RS30930 Genome accession   NZ_CP014035
Coordinates   105367..106236 (-) Length   289 a.a.
NCBI ID   WP_024374403.1    Uniprot ID   A0AAX2LNE1
Organism   Vibrio fluvialis strain ATCC 33809     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100367..111236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL536_RS30910 (AL536_08130) parC 101407..103680 (+) 2274 WP_047459105.1 DNA topoisomerase IV subunit A -
  AL536_RS30915 (AL536_08135) yacG 103731..103928 (-) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  AL536_RS30920 (AL536_08140) zapD 103983..104723 (-) 741 WP_020332196.1 cell division protein ZapD -
  AL536_RS30925 (AL536_08145) coaE 104756..105364 (-) 609 WP_061056140.1 dephospho-CoA kinase -
  AL536_RS30930 (AL536_08150) pilD 105367..106236 (-) 870 WP_024374403.1 prepilin peptidase Machinery gene
  AL536_RS30935 (AL536_08155) pilC 106288..107514 (-) 1227 WP_061056141.1 type II secretion system F family protein Machinery gene
  AL536_RS30940 (AL536_08160) pilB 107550..109238 (-) 1689 WP_061056142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL536_RS30945 (AL536_08165) pilA 109248..109667 (-) 420 WP_047459095.1 pilin Machinery gene
  AL536_RS30950 (AL536_08170) nadC 109898..110785 (-) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 289 a.a.        Molecular weight: 32248.27 Da        Isoelectric Point: 6.4655

>NTDB_id=167677 AL536_RS30930 WP_024374403.1 105367..106236(-) (pilD) [Vibrio fluvialis strain ATCC 33809]
MDIFNFYPWLFPTLATVLGLIIGSFLNVVIYRLPKIMEREWRQECAESFPEYHIEPPQGILTLSTPRSTCPHCDTPIRVI
DNIPVLSWLLLRGKCSNCQGSISARYPLIELLTASCSLLIALQFGFSWFAVAMLFFTYTLIAATFIDFDTMLLPDQLTLP
LLWAGIALALAGISPISLQDAVIGAIAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQQLPIIILMSSLVGL
VFGLIQLRLQKRGIDKAFPFGPYLAIAGWLSLLFGHDIAGWYFHSVLGF

Nucleotide


Download         Length: 870 bp        

>NTDB_id=167677 AL536_RS30930 WP_024374403.1 105367..106236(-) (pilD) [Vibrio fluvialis strain ATCC 33809]
ATGGACATTTTTAATTTTTACCCCTGGCTGTTTCCTACGCTGGCGACAGTGCTGGGGTTAATTATCGGCAGTTTCCTCAA
TGTGGTGATTTATCGCCTGCCGAAAATCATGGAGCGCGAGTGGCGTCAGGAGTGTGCGGAATCCTTTCCCGAGTATCATA
TTGAACCGCCTCAGGGCATTTTAACGCTGAGCACGCCACGTTCCACCTGCCCGCATTGCGATACGCCGATCCGGGTTATC
GACAACATTCCGGTACTGAGCTGGCTTTTGCTGCGTGGTAAATGCTCGAATTGTCAGGGCTCTATTAGCGCGCGTTATCC
ACTGATTGAACTGCTGACGGCTTCATGCAGCCTCTTGATTGCGCTGCAATTTGGTTTTAGCTGGTTTGCCGTTGCCATGC
TGTTCTTTACCTATACGCTGATTGCAGCAACCTTCATTGATTTCGATACGATGCTGCTGCCGGATCAGCTTACTCTGCCC
CTACTCTGGGCTGGTATTGCACTGGCATTGGCGGGCATCAGCCCGATTTCGCTACAAGATGCGGTGATTGGTGCGATAGC
GGGTTATCTGTCGTTATGGTCGGTGTACTGGCTGTTTAAGCTACTGACAGGCAAAGAAGGCATGGGCTATGGCGACTTTA
AGCTGTTAGCGGCTCTCGGTGCTTGGCTGGGATGGCAGCAACTACCGATCATCATTCTGATGTCATCTCTGGTTGGACTG
GTGTTCGGCCTCATTCAACTGCGTCTGCAAAAGCGCGGCATTGATAAAGCATTTCCCTTCGGGCCTTATCTGGCGATTGC
AGGCTGGCTGTCTCTGTTGTTTGGTCATGACATTGCGGGTTGGTATTTTCATTCGGTATTAGGATTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(135-244)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

78.472

99.654

0.782
  pilD Vibrio campbellii strain DS40M4

77.778

99.654

0.775
  pilD Acinetobacter baumannii D1279779

52.92

94.81

0.502
  pilD Acinetobacter nosocomialis M2

52.555

94.81

0.498
  pilD Neisseria gonorrhoeae MS11

50.929

93.08

0.474

Multiple sequence alignment