Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AL536_RS30940 Genome accession   NZ_CP014035
Coordinates   107550..109238 (-) Length   562 a.a.
NCBI ID   WP_061056142.1    Uniprot ID   -
Organism   Vibrio fluvialis strain ATCC 33809     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 102550..114238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL536_RS30915 (AL536_08135) yacG 103731..103928 (-) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  AL536_RS30920 (AL536_08140) zapD 103983..104723 (-) 741 WP_020332196.1 cell division protein ZapD -
  AL536_RS30925 (AL536_08145) coaE 104756..105364 (-) 609 WP_061056140.1 dephospho-CoA kinase -
  AL536_RS30930 (AL536_08150) pilD 105367..106236 (-) 870 WP_024374403.1 prepilin peptidase Machinery gene
  AL536_RS30935 (AL536_08155) pilC 106288..107514 (-) 1227 WP_061056141.1 type II secretion system F family protein Machinery gene
  AL536_RS30940 (AL536_08160) pilB 107550..109238 (-) 1689 WP_061056142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AL536_RS30945 (AL536_08165) pilA 109248..109667 (-) 420 WP_047459095.1 pilin Machinery gene
  AL536_RS30950 (AL536_08170) nadC 109898..110785 (-) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AL536_RS30955 (AL536_08175) ampD 110938..111489 (+) 552 WP_061056144.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AL536_RS30960 (AL536_08180) fldB 111494..112015 (-) 522 WP_020430452.1 flavodoxin FldB -
  AL536_RS30965 (AL536_08185) xerD 112156..113064 (+) 909 WP_047459088.1 site-specific tyrosine recombinase XerD -
  AL536_RS30970 (AL536_08190) dsbC 113081..113845 (+) 765 WP_061056145.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61932.92 Da        Isoelectric Point: 6.2639

>NTDB_id=167679 AL536_RS30940 WP_061056142.1 107550..109238(-) (pilB) [Vibrio fluvialis strain ATCC 33809]
MLTNLLAILRQADLLSPTQEQAVMDHIHASGTNVPEALIDLAIFHHHELTEHLSQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRCQALPLNSNGQILTLAVSDPTHLQVEDEFRFATGLQIELVLADHKALQAAIRRLYGRAIASHGTLGKEISQDELAS
LVQVSDDEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVEIQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAESVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=167679 AL536_RS30940 WP_061056142.1 107550..109238(-) (pilB) [Vibrio fluvialis strain ATCC 33809]
ATGCTCACCAACCTACTCGCCATCCTGCGTCAGGCCGATTTGCTTAGCCCTACGCAGGAACAGGCCGTAATGGATCATAT
TCACGCTTCCGGAACCAATGTTCCGGAAGCGTTGATCGATTTGGCGATCTTCCATCACCATGAACTGACCGAGCATCTAA
GCCAGATCTTTGGTCTGCCGAATACGGATCTGACCCGTTTTGACTTCGCGCCGCTTTGCCAGCAGTTGGGGCTGCGAGAG
CTCATCACCCGTTGTCAGGCTCTGCCCCTCAATAGCAACGGCCAGATACTGACTCTTGCGGTATCCGATCCTACTCACCT
GCAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAATAGAACTGGTATTGGCTGATCATAAGGCACTGCAAGCCG
CTATTCGCCGCTTGTATGGCCGAGCCATCGCATCACATGGCACGCTGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAAGTTTCCGATGACGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTCATCAA
CCAGATCCTGCTGGATGCGGTGCGTAAAGGCGCTTCTGATATCCACTTTGAGCCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGTATTTTGGTGGAAATCCAGCAACCAGCTAGCCATTTAAGCCGTCGATTGGCCGCGCGGATTAAGATT
TTGTCGAAACTCGATATCGCTGAGCGACGTTTACCCCAGGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTCTGCTCGACAGCAGTGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAACACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCCTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACACTGCACACCAACTCCGCCGCCGAAAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTTAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GGCGCCTTTGCCCTCACTGCAAAGTCGCCACATCGCCCTCTCCGACACTGCAACAACAATTTCACTTGAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATTCATCAGATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATTACCAGTTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.384

100

0.824
  pilB Vibrio campbellii strain DS40M4

72.598

100

0.726
  pilB Vibrio parahaemolyticus RIMD 2210633

71.708

100

0.717
  pilB Legionella pneumophila strain ERS1305867

52.529

91.459

0.48
  pilB Acinetobacter baumannii D1279779

50.096

92.349

0.463
  pilB Acinetobacter baylyi ADP1

48.184

93.06

0.448
  pilF Neisseria gonorrhoeae MS11

44.326

100

0.445
  pilF Thermus thermophilus HB27

37.367

100

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

37.455

97.865

0.367

Multiple sequence alignment