Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   APT60_RS23825 Genome accession   NZ_CP013989
Coordinates   5181258..5181707 (-) Length   149 a.a.
NCBI ID   WP_003112842.1    Uniprot ID   P04739
Organism   Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5176258..5186707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APT60_RS23805 (APT60_23805) ampD 5176950..5177516 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  APT60_RS23810 (APT60_23810) - 5177667..5179922 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  APT60_RS23815 (APT60_23815) nadC 5180208..5181056 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  APT60_RS23825 (APT60_23825) pilA 5181258..5181707 (-) 450 WP_003112842.1 type 4a pilus biogenesis protein PilA Machinery gene
  APT60_RS23830 (APT60_23830) pilB 5181939..5183639 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  APT60_RS23835 (APT60_23835) pilC 5183643..5184863 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  APT60_RS23840 (APT60_23840) pilD 5184867..5185739 (+) 873 WP_059349755.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  APT60_RS23845 (APT60_23845) coaE 5185736..5186347 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  APT60_RS23850 (APT60_23850) yacG 5186344..5186544 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15511.78 Da        Isoelectric Point: 6.2905

>NTDB_id=166927 APT60_RS23825 WP_003112842.1 5181258..5181707(-) (pilA) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV
GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=166927 APT60_RS23825 WP_003112842.1 5181258..5181707(-) (pilA) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC
GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1PAN
  PDB 1PAO
  PDB 8TUM
  PDB 8TUW
  PDB 9EWX

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

67.785

100

0.678
  pilA Acinetobacter baumannii strain A118

48

100

0.483
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.322

100

0.483
  pilA Vibrio cholerae strain A1552

48.322

100

0.483
  pilA Vibrio cholerae C6706

48.322

100

0.483
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.638

100

0.456
  pilA Vibrio parahaemolyticus RIMD 2210633

44.898

98.658

0.443
  comP Acinetobacter baylyi ADP1

41.83

100

0.43
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.056

95.973

0.423
  pilA2 Legionella pneumophila str. Paris

38.776

98.658

0.383
  pilA2 Legionella pneumophila strain ERS1305867

38.095

98.658

0.376

Multiple sequence alignment