Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   APT60_RS23830 Genome accession   NZ_CP013989
Coordinates   5181939..5183639 (+) Length   566 a.a.
NCBI ID   WP_003112841.1    Uniprot ID   P22608
Organism   Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5176939..5188639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  APT60_RS23805 (APT60_23805) ampD 5176950..5177516 (-) 567 WP_003104911.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  APT60_RS23810 (APT60_23810) - 5177667..5179922 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  APT60_RS23815 (APT60_23815) nadC 5180208..5181056 (+) 849 WP_003122081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  APT60_RS23825 (APT60_23825) pilA 5181258..5181707 (-) 450 WP_003112842.1 type 4a pilus biogenesis protein PilA Machinery gene
  APT60_RS23830 (APT60_23830) pilB 5181939..5183639 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  APT60_RS23835 (APT60_23835) pilC 5183643..5184863 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  APT60_RS23840 (APT60_23840) pilD 5184867..5185739 (+) 873 WP_059349755.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  APT60_RS23845 (APT60_23845) coaE 5185736..5186347 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  APT60_RS23850 (APT60_23850) yacG 5186344..5186544 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  APT60_RS23855 (APT60_23855) - 5186581..5186790 (-) 210 WP_003094660.1 hypothetical protein -
  APT60_RS23860 (APT60_23860) - 5186896..5187585 (-) 690 WP_034034896.1 energy-coupling factor ABC transporter permease -
  APT60_RS23865 (APT60_23865) - 5187582..5188052 (-) 471 WP_003103866.1 hypothetical protein -
  APT60_RS23870 (APT60_23870) - 5188049..5188474 (-) 426 WP_059349756.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62328.19 Da        Isoelectric Point: 5.7041

>NTDB_id=166928 APT60_RS23830 WP_003112841.1 5181939..5183639(+) (pilB) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQSKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=166928 APT60_RS23830 WP_003112841.1 5181939..5183639(+) (pilB) [Pseudomonas aeruginosa strain USDA-ARS-USMARC-41639]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAGCAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTTGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P22608

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.517

100

0.581
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.647

0.509
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio cholerae strain A1552

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.823

99.647

0.496
  pilF Thermus thermophilus HB27

39.75

99.117

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366

Multiple sequence alignment