Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AOT82_RS06915 Genome accession   NZ_CP012969
Coordinates   1649723..1650844 (+) Length   373 a.a.
NCBI ID   WP_071002369.1    Uniprot ID   A0A1G6U944
Organism   Psychrobacter sp. AntiMn-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1644723..1655844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT82_RS06900 (AOT82_1465) - 1646079..1647353 (+) 1275 WP_071002367.1 hemolysin family protein -
  AOT82_RS06905 (AOT82_1466) - 1647454..1648182 (-) 729 WP_071003702.1 YggS family pyridoxal phosphate-dependent enzyme -
  AOT82_RS06910 (AOT82_1467) pilT 1648548..1649603 (+) 1056 WP_071002368.1 type IV pilus twitching motility protein PilT Machinery gene
  AOT82_RS06915 (AOT82_1468) pilU 1649723..1650844 (+) 1122 WP_071002369.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AOT82_RS06920 (AOT82_1469) fur 1650981..1651415 (-) 435 WP_058024231.1 ferric iron uptake transcriptional regulator -
  AOT82_RS06925 (AOT82_1470) bamE 1651743..1652162 (+) 420 WP_058024230.1 outer membrane protein assembly factor BamE -
  AOT82_RS06930 (AOT82_1471) - 1652213..1652596 (-) 384 WP_227517114.1 RnfH family protein -
  AOT82_RS06935 (AOT82_1472) - 1652572..1653702 (-) 1131 WP_071002371.1 hypothetical protein -
  AOT82_RS06940 (AOT82_1473) - 1653994..1654662 (-) 669 WP_071002372.1 c-type cytochrome -
  AOT82_RS06945 (AOT82_1474) - 1654986..1655321 (-) 336 WP_058024226.1 c-type cytochrome -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41619.92 Da        Isoelectric Point: 7.1147

>NTDB_id=158660 AOT82_RS06915 WP_071002369.1 1649723..1650844(+) (pilU) [Psychrobacter sp. AntiMn-1]
MDIDKLLQLMINKNGSDLFITADVAPSMKVNGKILPVGKTPLTADQTMMLVKGVMTPSQQKEFKETNECQFAISDEAQQA
RFRVSAFIQRDMAGMILRKIENKIPTVEELRLPPALKELAMKKRGIILLVGATGTGKSSTLAAMIGHRNQNSHGHIITIE
DPIEFVHKHRGCIITQREVGIDTHSFEAGLKNTLRQAPDVILIGEIRNREVMSYAIQYAETGHLVFATLHANNANQAIDR
IIHFFETSRHNQLFMDLSLNLQAIIAQQLIPTPDGKGRRAAIEILLNSQLISDYIRKGEVHEIKDVMTRSRDSGMQTFDQ
ALFDLYEQGEITYKEAILHADSPNDLRLKIKLSSKNGKANLDEGADSLSLDIS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=158660 AOT82_RS06915 WP_071002369.1 1649723..1650844(+) (pilU) [Psychrobacter sp. AntiMn-1]
ATGGACATTGATAAATTATTGCAGTTGATGATTAATAAAAATGGCTCTGACCTTTTTATTACTGCTGATGTAGCGCCTTC
CATGAAAGTAAATGGAAAAATTTTACCTGTCGGTAAAACCCCTTTGACCGCTGATCAAACCATGATGTTAGTCAAAGGAG
TGATGACCCCTAGCCAGCAAAAAGAATTTAAAGAGACGAACGAGTGTCAATTTGCTATATCTGATGAAGCACAGCAAGCA
CGCTTTCGGGTCAGTGCATTCATACAGCGCGATATGGCAGGAATGATTTTACGGAAAATTGAGAATAAGATCCCAACTGT
TGAGGAACTACGCTTGCCGCCAGCGCTCAAAGAACTGGCCATGAAAAAGCGCGGCATTATCTTGCTGGTCGGCGCGACCG
GCACAGGTAAATCCTCTACGCTCGCGGCTATGATTGGACATCGTAACCAAAACTCTCATGGGCATATCATTACCATAGAA
GACCCTATCGAGTTTGTCCATAAACATCGTGGTTGTATCATCACTCAGCGTGAAGTCGGTATCGACACTCATTCATTTGA
AGCAGGACTAAAAAATACGTTGCGCCAAGCACCAGATGTCATTTTGATTGGTGAGATTCGTAACCGTGAGGTCATGAGCT
ACGCTATCCAGTACGCTGAGACAGGGCATCTGGTATTTGCAACCTTGCATGCCAATAATGCCAACCAAGCGATTGACCGT
ATTATTCATTTCTTTGAAACCAGTCGTCATAATCAGCTATTTATGGATTTGTCACTGAACTTGCAGGCCATTATCGCTCA
GCAGCTCATTCCAACCCCTGATGGCAAAGGACGCCGTGCTGCTATTGAGATTTTATTAAACTCACAGCTAATTAGCGACT
ATATCCGTAAAGGTGAGGTGCATGAAATCAAAGACGTCATGACACGCTCTCGAGACAGCGGTATGCAAACCTTTGATCAA
GCGCTTTTTGACTTGTATGAACAAGGAGAGATCACTTATAAAGAGGCAATTTTGCATGCTGACTCCCCTAACGACTTACG
CCTAAAAATTAAGCTCAGTAGCAAAAACGGTAAGGCTAATTTAGATGAAGGTGCGGATAGTTTATCTTTAGACATCAGTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G6U944

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.488

100

0.665
  pilU Acinetobacter baylyi ADP1

66.48

95.979

0.638
  pilU Vibrio cholerae strain A1552

52.717

98.66

0.52
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.53

98.123

0.408
  pilT Legionella pneumophila strain Lp02

40.774

90.08

0.367
  pilT Legionella pneumophila strain ERS1305867

40.774

90.08

0.367
  pilT Acinetobacter nosocomialis M2

39.65

91.957

0.365
  pilT Acinetobacter baumannii strain A118

39.65

91.957

0.365
  pilT Acinetobacter baumannii D1279779

39.65

91.957

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.299

89.812

0.362
  pilT Vibrio cholerae strain A1552

40.299

89.812

0.362
  pilT Pseudomonas aeruginosa PAK

39.017

92.761

0.362

Multiple sequence alignment