Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB/cilA   Type   Machinery gene
Locus tag   RN86_RS04255 Genome accession   NZ_CP012648
Coordinates   858736..859131 (-) Length   131 a.a.
NCBI ID   WP_046164720.1    Uniprot ID   A0A0F5MM81
Organism   Streptococcus gordonii strain KCOM 1506 (= ChDC B679)     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 856211..879902 858736..859131 within 0


Gene organization within MGE regions


Location: 856211..879902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RN86_RS04240 (RN86_04235) - 856211..856414 (+) 204 WP_046164722.1 helix-turn-helix transcriptional regulator -
  RN86_RS11460 - 856411..856575 (+) 165 WP_082730221.1 hypothetical protein -
  RN86_RS04245 (RN86_04240) - 856585..857019 (+) 435 WP_046164721.1 hypothetical protein -
  RN86_RS04250 (RN86_04245) - 857073..858557 (-) 1485 WP_053794892.1 DUF1846 domain-containing protein -
  RN86_RS04255 (RN86_04250) ssbB/cilA 858736..859131 (-) 396 WP_046164720.1 single-stranded DNA-binding protein Machinery gene
  RN86_RS04260 (RN86_04255) ytpR 859195..859821 (-) 627 WP_046164719.1 YtpR family tRNA-binding protein -
  RN86_RS04265 (RN86_04260) - 859838..860155 (-) 318 WP_046164718.1 thioredoxin family protein -
  RN86_RS04270 (RN86_04265) - 860155..860439 (-) 285 WP_053794333.1 DUF4651 domain-containing protein -
  RN86_RS04275 (RN86_04270) pepA 860620..861687 (+) 1068 WP_053794334.1 glutamyl aminopeptidase -
  RN86_RS04280 (RN86_04275) - 861936..862922 (+) 987 WP_125377546.1 substrate-binding domain-containing protein -
  RN86_RS04285 (RN86_04280) - 862922..864235 (+) 1314 WP_046164715.1 sensor histidine kinase -
  RN86_RS04290 (RN86_04285) - 864228..864911 (+) 684 WP_002896732.1 response regulator transcription factor -
  RN86_RS04295 (RN86_04290) - 864908..866191 (+) 1284 WP_053794335.1 sugar ABC transporter substrate-binding protein -
  RN86_RS04300 (RN86_04295) - 866408..866845 (+) 438 WP_053794336.1 PTS fructose transporter subunit IIA -
  RN86_RS04305 (RN86_04300) - 866866..867360 (+) 495 WP_008810035.1 PTS sugar transporter subunit IIB -
  RN86_RS04310 (RN86_04305) - 867391..868242 (+) 852 WP_008810034.1 PTS sugar transporter subunit IIC -
  RN86_RS04315 (RN86_04310) - 868244..869074 (+) 831 WP_048777245.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
  RN86_RS04320 (RN86_04315) - 869086..869376 (+) 291 WP_046164712.1 hypothetical protein -
  RN86_RS04325 (RN86_04320) - 869779..871752 (+) 1974 WP_046164711.1 ABC transporter substrate binding protein -
  RN86_RS04330 (RN86_04325) - 871752..872555 (+) 804 WP_046164710.1 ATP-binding cassette domain-containing protein -
  RN86_RS04335 (RN86_04330) groES 872716..872997 (+) 282 WP_012130907.1 co-chaperone GroES -
  RN86_RS04340 (RN86_04335) groL 873020..874642 (+) 1623 WP_045634142.1 chaperonin GroEL -
  RN86_RS04345 (RN86_04340) - 874766..875524 (+) 759 WP_045634141.1 epoxyqueuosine reductase QueH -
  RN86_RS04350 (RN86_04345) - 875618..876439 (-) 822 WP_046164708.1 PrsW family glutamic-type intramembrane protease -
  RN86_RS04355 (RN86_04350) folE 876487..877050 (-) 564 WP_008810025.1 GTP cyclohydrolase I FolE -
  RN86_RS04360 (RN86_04355) rpsF 877262..877552 (+) 291 WP_002896497.1 30S ribosomal protein S6 -
  RN86_RS04365 (RN86_04360) ssb 877569..878060 (+) 492 WP_046164707.1 single-stranded DNA-binding protein Machinery gene
  RN86_RS04370 (RN86_04365) rpsR 878099..878338 (+) 240 WP_000068664.1 30S ribosomal protein S18 -
  RN86_RS04375 (RN86_04370) - 878392..879048 (+) 657 WP_080998581.1 TetR/AcrR family transcriptional regulator -
  RN86_RS04380 (RN86_04375) - 879189..879902 (+) 714 WP_046164705.1 ABC transporter ATP-binding protein -

Sequence


Protein


Download         Length: 131 a.a.        Molecular weight: 14776.67 Da        Isoelectric Point: 6.2467

>NTDB_id=156078 RN86_RS04255 WP_046164720.1 858736..859131(-) (ssbB/cilA) [Streptococcus gordonii strain KCOM 1506 (= ChDC B679)]
MYNKVIIIGRLTATPELHKTSNEKSVTRATIAVNRRFKDQNGEREADFINIVLWGKLAETLASYASKGSLISLDGELRTR
RYEKDGVTHYVTEVLGHGFQLLESRAQRAIRENNSGADLADLVLEEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=156078 RN86_RS04255 WP_046164720.1 858736..859131(-) (ssbB/cilA) [Streptococcus gordonii strain KCOM 1506 (= ChDC B679)]
ATGTACAATAAAGTCATTATTATCGGTCGTTTGACGGCCACACCAGAATTGCACAAAACTAGCAATGAAAAATCTGTCAC
ACGCGCAACAATTGCTGTTAACCGTCGTTTTAAGGACCAAAATGGTGAACGTGAAGCAGACTTTATCAATATCGTCCTTT
GGGGCAAATTGGCTGAGACTCTAGCTAGTTATGCCAGCAAAGGAAGCTTAATTTCTCTGGACGGCGAACTACGGACCCGT
CGTTATGAAAAAGACGGTGTGACTCACTATGTCACAGAAGTCCTTGGACATGGTTTCCAGCTTCTTGAAAGTCGTGCCCA
GCGGGCTATTCGTGAAAATAATTCTGGAGCCGATTTGGCTGACCTAGTCTTGGAAGAAGAGGAACTTCCTTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0F5MM81

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB/cilA Streptococcus mitis NCTC 12261

83.969

100

0.84

  ssbB/cilA Streptococcus pneumoniae Rx1

83.969

100

0.84

  ssbB/cilA Streptococcus pneumoniae D39

83.969

100

0.84

  ssbB/cilA Streptococcus pneumoniae R6

83.969

100

0.84

  ssbB/cilA Streptococcus pneumoniae TIGR4

83.969

100

0.84

  ssbB/cilA Streptococcus mitis SK321

83.206

100

0.832

  ssbA Streptococcus mutans UA159

81.679

100

0.817

  ssbB Streptococcus sobrinus strain NIDR 6715-7

75.573

100

0.756

  ssbB Lactococcus lactis subsp. cremoris KW2

63.393

85.496

0.542

  ssb Latilactobacillus sakei subsp. sakei 23K

50.943

80.916

0.412

  ssbA Bacillus subtilis subsp. subtilis str. 168

50

80.916

0.405

  ssbB Bacillus subtilis subsp. subtilis str. 168

45.37

82.443

0.374


Multiple sequence alignment