Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PA8281_RS25870 Genome accession   NZ_CP015002
Coordinates   5554826..5555299 (-) Length   157 a.a.
NCBI ID   WP_019727264.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PA8281     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5549826..5560299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PA8281_RS25850 (PA8281_02568) ampD 5550567..5551133 (-) 567 WP_009875882.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PA8281_RS25855 (PA8281_02569) - 5551284..5553539 (-) 2256 WP_019726810.1 DUF1631 domain-containing protein -
  PA8281_RS25860 (PA8281_02570) nadC 5553825..5554673 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PA8281_RS25870 (PA8281_02571) pilE 5554826..5555299 (-) 474 WP_019727264.1 pilin Machinery gene
  PA8281_RS25875 (PA8281_02572) pilB 5555530..5557230 (+) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PA8281_RS25880 (PA8281_02573) pilC 5557234..5558451 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  PA8281_RS25885 (PA8281_02574) pilD 5558454..5559326 (+) 873 WP_019726811.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PA8281_RS25890 (PA8281_02575) coaE 5559323..5559934 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  PA8281_RS25895 (PA8281_02576) yacG 5559931..5560131 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16162.51 Da        Isoelectric Point: 8.0955

>NTDB_id=150887 PA8281_RS25870 WP_019727264.1 5554826..5555299(-) (pilE) [Pseudomonas aeruginosa strain PA8281]
MKAAQKGFTLIELMIVVAIIGILAAIAIPAYQDYTARAQLSEAMTLASGLKTKVSDIFSQDGSCPANTAATAGIEKDTDI
NGKYVAKVTTGGTAAASGGCTIVATMKASDVATPLREKTLTLTLGNADKGSYTWACTSNADNKYLPKTCQTATTTTP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=150887 PA8281_RS25870 WP_019727264.1 5554826..5555299(-) (pilE) [Pseudomonas aeruginosa strain PA8281]
ATGAAAGCTGCTCAAAAAGGCTTTACCTTGATCGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCTGCCATCGC
TATTCCAGCGTATCAGGACTACACCGCACGCGCTCAGCTTAGCGAAGCCATGACCCTGGCCAGTGGTCTCAAGACGAAAG
TGAGCGATATCTTCTCTCAGGATGGGTCCTGCCCGGCTAATACTGCTGCCACGGCAGGCATCGAGAAAGATACCGACATC
AACGGCAAGTATGTTGCCAAGGTAACAACTGGTGGCACCGCAGCTGCGTCTGGTGGTTGCACTATCGTTGCTACTATGAA
AGCCTCTGATGTGGCTACTCCTCTGAGGGAGAAAACTCTGACTTTGACTCTAGGAAATGCTGACAAGGGTTCTTACACTT
GGGCCTGTACTTCCAACGCAGATAACAAGTACCTGCCAAAAACCTGCCAGACTGCTACCACTACCACTCCGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-150)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

42.197

100

0.465
  comP Acinetobacter baylyi ADP1

46.405

97.452

0.452
  pilE Neisseria gonorrhoeae MS11

41.916

100

0.446
  pilA/pilA1 Eikenella corrodens VA1

40.741

100

0.42
  pilA2 Legionella pneumophila str. Paris

44.898

93.631

0.42
  pilA2 Legionella pneumophila strain ERS1305867

43.421

96.815

0.42
  pilA Ralstonia pseudosolanacearum GMI1000

40.252

100

0.408
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.667

95.541

0.408
  pilA Pseudomonas aeruginosa PAK

41.06

96.178

0.395
  pilA Acinetobacter nosocomialis M2

55.357

71.338

0.395
  pilA Acinetobacter baumannii strain A118

37.5

96.815

0.363