Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACP86_RS12580 Genome accession   NZ_CP011929
Coordinates   2729461..2730582 (+) Length   373 a.a.
NCBI ID   WP_008174191.1    Uniprot ID   G6YV23
Organism   Marinobacter sp. CP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2724461..2735582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACP86_RS12560 (ACP86_12555) - 2726021..2726605 (-) 585 WP_008174183.1 YggT family protein -
  ACP86_RS12565 (ACP86_12560) proC 2726628..2727461 (-) 834 WP_014575886.1 pyrroline-5-carboxylate reductase -
  ACP86_RS12570 (ACP86_12565) - 2727523..2728227 (-) 705 WP_039882033.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACP86_RS12575 (ACP86_12570) pilT 2728368..2729402 (+) 1035 WP_008174189.1 type IV pilus twitching motility protein PilT Machinery gene
  ACP86_RS12580 (ACP86_12575) pilU 2729461..2730582 (+) 1122 WP_008174191.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACP86_RS12585 (ACP86_12580) - 2730822..2732105 (+) 1284 WP_008174193.1 OmpP1/FadL family transporter -
  ACP86_RS12590 (ACP86_12585) - 2732137..2732511 (-) 375 WP_053114212.1 hypothetical protein -
  ACP86_RS12595 (ACP86_12590) polA 2732742..2735474 (-) 2733 WP_053114214.1 DNA polymerase I -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41500.65 Da        Isoelectric Point: 6.5039

>NTDB_id=149352 ACP86_RS12580 WP_008174191.1 2729461..2730582(+) (pilU) [Marinobacter sp. CP1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRAEFEETHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLSLPEVIKELAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLKAIVAQQLVPTPDGKGRKAVIEVLINTPLVSDLIRKGEVHRLKELMSKSNESGMQTFDQA
LYQLYAEGSITYEDALAHADSANDLRLMIKLGADAQGADKLSSSVDKLSIQDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=149352 ACP86_RS12580 WP_008174191.1 2729461..2730582(+) (pilU) [Marinobacter sp. CP1]
ATGGAATTCGAAAAACTGTTACGGCTGATGGTGGAGAAGGGCGGTTCGGACCTGTTTATTACAGCGGGTGTGCCGCCGAG
TATGAAGGTGAACGGGAAGGTTCTACCGGTCACCAAGAACGCGTTGACGCCGGAGCAGACCAGGGAGTTCGTTTACGGCG
CCATGAACGACAAGCAGCGCGCCGAATTCGAGGAAACCCATGAGTGTAACTTCGCTATCAGTGCCCGTGGCATTGGCCGT
TTCCGTGTTTCTGCCTTTTTCCAGCGCAACCTGTGCGGGATGGTGCTGCGTCGGATCGAGGTAAAGATCCCGCAAATCGA
TGATCTGTCGTTGCCGGAGGTAATCAAGGAACTGGCCATGACCAAGCGTGGCCTGATCATGTTCGTGGGCGCTACCGGTA
CCGGCAAGTCTACGTCCCTTGCGGCCATGCTCGGGCATCGTAACCGCAACAGCCGTGGCCACATCATTTCCATCGAAGAC
CCGATCGAATTCGTGCACCAGCATCAGGGTTGTATCGTCACCCAGCGCGAGGTGGGTATCGACACCGAGAGCTTTGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCACCAGACGTTATTCTGATCGGTGAGGTTCGTACCCGGCAGACCATGGAATATT
CCGTGCAATTCGCCGAGACCGGTCACCTGTGTCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCCAGTTCTTCCCGCCGGAACAGCACAACCAGATCTGGATGGACCTGTCCCTGAACCTCAAGGCGATCGTGGCTCAGCA
GCTGGTGCCAACGCCAGATGGCAAGGGCCGTAAGGCGGTTATCGAGGTGCTGATCAATACCCCGCTGGTATCGGACCTCA
TCCGGAAGGGCGAGGTGCATCGGCTGAAAGAGCTGATGTCCAAATCCAACGAATCCGGCATGCAGACCTTTGACCAGGCG
CTTTATCAGCTCTATGCAGAAGGGTCGATTACTTATGAGGACGCCCTGGCTCATGCTGATTCTGCTAACGACCTGCGCCT
GATGATCAAACTGGGTGCCGACGCCCAGGGAGCCGACAAGCTCTCGTCTTCCGTCGACAAGCTTTCCATTCAGGATGACT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G6YV23

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

79.245

99.464

0.788
  pilU Acinetobacter baylyi ADP1

64.888

95.442

0.619
  pilU Vibrio cholerae strain A1552

57.297

99.196

0.568
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

90.885

0.386
  pilT Pseudomonas aeruginosa PAK

40.896

89.812

0.367
  pilT Acinetobacter baumannii strain A118

40.597

89.812

0.365
  pilT Acinetobacter baumannii D1279779

40.597

89.812

0.365
  pilT Pseudomonas stutzeri DSM 10701

40.597

89.812

0.365
  pilT Acinetobacter nosocomialis M2

40.299

89.812

0.362
  pilT Legionella pneumophila strain Lp02

40.299

89.812

0.362
  pilT Legionella pneumophila strain ERS1305867

40.299

89.812

0.362

Multiple sequence alignment