Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WQ53_RS11045 Genome accession   NZ_CP011144
Coordinates   2666733..2667869 (-) Length   378 a.a.
NCBI ID   WP_052632357.1    Uniprot ID   A0A0E3UNS5
Organism   Pseudoxanthomonas suwonensis strain J1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2661733..2672869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WQ53_RS11020 (WQ53_11085) - 2661874..2662263 (-) 390 WP_052632350.1 phasin family protein -
  WQ53_RS11025 (WQ53_11090) queD 2662499..2662852 (-) 354 WP_052632352.1 6-carboxytetrahydropterin synthase QueD -
  WQ53_RS11030 (WQ53_11095) - 2662888..2664249 (-) 1362 WP_052632354.1 DEAD/DEAH box helicase -
  WQ53_RS11035 (WQ53_11100) - 2664746..2665744 (+) 999 WP_052632356.1 fumarylacetoacetate hydrolase family protein -
  WQ53_RS11040 (WQ53_11105) maiA 2665853..2666521 (+) 669 WP_052634052.1 maleylacetoacetate isomerase -
  WQ53_RS11045 (WQ53_11110) pilU 2666733..2667869 (-) 1137 WP_052632357.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WQ53_RS11050 (WQ53_11115) - 2667976..2668374 (+) 399 WP_052632359.1 DUF4398 domain-containing protein -
  WQ53_RS11055 (WQ53_11120) - 2668371..2669252 (+) 882 WP_052632361.1 hypothetical protein -
  WQ53_RS16855 - 2669444..2669638 (+) 195 WP_144409313.1 hypothetical protein -
  WQ53_RS11060 (WQ53_11125) - 2669656..2669871 (+) 216 WP_052632363.1 YdcH family protein -
  WQ53_RS11065 (WQ53_11130) - 2670066..2671442 (+) 1377 WP_052632365.1 pyridoxal-phosphate dependent enzyme -
  WQ53_RS11070 (WQ53_11135) - 2671655..2672848 (+) 1194 WP_052632367.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41855.33 Da        Isoelectric Point: 6.2370

>NTDB_id=142700 WQ53_RS11045 WP_052632357.1 2666733..2667869(-) (pilU) [Pseudoxanthomonas suwonensis strain J1]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEDLNLPDVLKDIIMTPRGLVLLVGSTGSGKSTALASMIDHRNNSTTGHILTIED
PIEYLHRHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGIDERRLPATEVLINTPMIRDLLRRGQVHEVKQAMEASLEEGMQTFDQCL
FRMVKQGRIEQEEALRAADSRDGLALKFRLSEGADAEHDPYADVTTPPATPTITHGFV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=142700 WQ53_RS11045 WP_052632357.1 2666733..2667869(-) (pilU) [Pseudoxanthomonas suwonensis strain J1]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAGAAGAACGCCTCGGACATGTTCCTGACCACCGGTGCTCCGGTATA
CATCAAGATCGAAGGCAAGCTGTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTGAACATGGCCATTGCCCTGGCCGACGCCGGGCGC
TTCCGGGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGAGCATCGA
GGACCTCAACCTGCCGGACGTCCTCAAGGACATCATCATGACCCCGCGCGGGCTGGTGCTGCTGGTGGGCTCCACCGGCT
CGGGCAAGTCGACCGCGCTGGCGTCGATGATCGACCACCGCAACAACAGCACCACCGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGACCACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTGTCGCTGCG
CCTGGTCAAGGGTATAGATGAGCGCCGGCTGCCGGCCACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAGGCCTCGCTGGAGGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGCAGGGCCGGATCGAGCAGGAGGAGGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGCCGACGCCGAGCATGACCCCTATGCCGACGTGACCACCCCGCCGGCGACGCCGACGATCA
CCCACGGCTTCGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E3UNS5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.811

94.974

0.492
  pilU Acinetobacter baylyi ADP1

48.459

94.444

0.458
  pilU Vibrio cholerae strain A1552

47.191

94.18

0.444
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.767

91.005

0.407
  pilT Pseudomonas aeruginosa PAK

41.716

89.418

0.373
  pilT Legionella pneumophila strain ERS1305867

41.124

89.418

0.368
  pilT Legionella pneumophila strain Lp02

41.124

89.418

0.368
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.418

0.365
  pilT Acinetobacter baumannii D1279779

40.828

89.418

0.365
  pilT Acinetobacter nosocomialis M2

40.828

89.418

0.365
  pilT Acinetobacter baumannii strain A118

40.828

89.418

0.365
  pilT Acinetobacter baylyi ADP1

40.294

89.947

0.362

Multiple sequence alignment