Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AO356_RS16040 Genome accession   NZ_CP012831
Coordinates   3621257..3621667 (+) Length   136 a.a.
NCBI ID   WP_060743129.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3616257..3626667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS16015 (AO356_15995) yacG 3616407..3616613 (-) 207 WP_057450865.1 DNA gyrase inhibitor YacG -
  AO356_RS16020 (AO356_16000) coaE 3616610..3617233 (-) 624 WP_060740577.1 dephospho-CoA kinase -
  AO356_RS16025 (AO356_16005) pilD 3617233..3618102 (-) 870 WP_060740578.1 prepilin peptidase Machinery gene
  AO356_RS16030 (AO356_16010) pilC 3618104..3619321 (-) 1218 WP_060740579.1 type II secretion system F family protein Machinery gene
  AO356_RS16035 (AO356_16015) pilB 3619324..3621033 (-) 1710 WP_060740580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AO356_RS16040 (AO356_16020) pilA 3621257..3621667 (+) 411 WP_060743129.1 pilin Machinery gene
  AO356_RS16045 (AO356_16025) - 3621936..3622289 (+) 354 WP_053125323.1 BON domain-containing protein -
  AO356_RS16050 (AO356_16030) - 3622620..3622916 (-) 297 WP_060740581.1 DUF2845 domain-containing protein -
  AO356_RS16055 (AO356_16035) - 3622918..3625851 (-) 2934 WP_203225753.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13737.91 Da        Isoelectric Point: 8.1087

>NTDB_id=136754 AO356_RS16040 WP_060743129.1 3621257..3621667(+) (pilA) [Pseudomonas fluorescens strain FW300-N2C3]
MNKQTGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLGEISALKVPYEDMMNQGTSPTAANMNITTPTSNCAIA
VTGTAAAGTGTIVCTLSNAPAAVAGKTITLSRDAAGVWTCASNAAKEFLPAGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=136754 AO356_RS16040 WP_060743129.1 3621257..3621667(+) (pilA) [Pseudomonas fluorescens strain FW300-N2C3]
ATGAATAAGCAAACGGGCTTTACGCTGATCGAGCTGCTGATCGTCGTGGCGATCATTGGCATTCTGGCGACCTTTGCGTT
GCCGCAATATTCCAAATATCAGGCACGGGCCAAGGCTACGGCCGGGCTGGGGGAAATTTCTGCGTTGAAGGTACCGTACG
AGGACATGATGAATCAGGGGACCAGCCCGACGGCGGCCAACATGAACATCACGACACCTACCAGCAACTGCGCAATCGCA
GTCACGGGCACTGCCGCGGCAGGCACGGGGACCATCGTTTGTACCCTCTCGAATGCTCCTGCTGCCGTAGCGGGTAAGAC
AATCACCCTTAGTCGTGATGCCGCCGGCGTCTGGACATGCGCATCTAACGCTGCCAAGGAATTCCTGCCGGCTGGCTGCC
CTGGCGTCTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

47.445

100

0.478
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.118

100

0.441
  pilA Ralstonia pseudosolanacearum GMI1000

37.908

100

0.426
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.86

100

0.419
  pilA Vibrio cholerae strain A1552

39.86

100

0.419
  pilA Vibrio cholerae C6706

39.86

100

0.419
  pilA Pseudomonas aeruginosa PAK

37.838

100

0.412
  pilA/pilA1 Eikenella corrodens VA1

38.095

100

0.412
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.299

98.529

0.397
  comP Acinetobacter baylyi ADP1

36.242

100

0.397
  pilA2 Legionella pneumophila str. Paris

37.241

100

0.397
  pilA2 Legionella pneumophila strain ERS1305867

36.552

100

0.39

Multiple sequence alignment