Detailed information NTG0136350

Bioinformatically predicted

Overview

• Name: comGC/cglC
• Type: Machinery gene
• Locus tag: GBS85147_RS01000
• Genome accession: NZ_CP010319
• Coordinates: 183773..184102 (+)
• Length: 109 a.a.
• NCBI ID: WP_000793380.1
• Uniprot ID: A0A0E1EKJ1
• Organism: Streptococcus agalactiae strain GBS85147
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 178773..189102

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GBS85147_RS00985 (GBS85147_0199)	-	181331..181696 (+)	366	WP_000285373.1	DUF1033 family protein	-
GBS85147_RS00990 (GBS85147_0200)	comYA	181868..182839 (+)	972	WP_000250799.1	competence type IV pilus ATPase ComGA	Machinery gene
GBS85147_RS00995 (GBS85147_0201)	comYB	182733..183776 (+)	1044	WP_228322108.1	competence type IV pilus assembly protein ComGB	Machinery gene
GBS85147_RS01000 (GBS85147_0202)	comGC/cglC	183773..184102 (+)	330	WP_000793380.1	competence type IV pilus major pilin ComGC	Machinery gene
GBS85147_RS01005 (GBS85147_0203)	comYD	184077..184490 (+)	414	WP_017646959.1	competence type IV pilus minor pilin ComGD	Machinery gene
GBS85147_RS01010	comGE	184462..184761 (+)	300	WP_025195478.1	competence type IV pilus minor pilin ComGE	-
GBS85147_RS01015 (GBS85147_0204)	comGF	184715..185176 (+)	462	WP_001874060.1	competence type IV pilus minor pilin ComGF	-
GBS85147_RS01020 (GBS85147_0205)	comGG	185154..185525 (+)	372	WP_000601103.1	competence type IV pilus minor pilin ComGG	-
GBS85147_RS01025 (GBS85147_0206)	comYH	185640..186614 (+)	975	WP_001008568.1	class I SAM-dependent methyltransferase	Machinery gene
GBS85147_RS01030 (GBS85147_0207)	-	186646..187839 (+)	1194	WP_000047537.1	acetate kinase	-
GBS85147_RS01035 (GBS85147_0208)	-	187991..188197 (+)	207	WP_000798241.1	helix-turn-helix transcriptional regulator	-
GBS85147_RS09950	-	188256..188393 (+)	138	WP_079263416.1	hypothetical protein	-
GBS85147_RS01040 (GBS85147_0209)	-	188434..188889 (+)	456	WP_053039115.1	hypothetical protein	-

Sequence

Protein

• Length: 109 a.a.
• Molecular weight: 12129.27 Da
• Isoelectric Point: 10.0311

>NTDB_id=136350 GBS85147_RS01000 WP_000793380.1 183773..184102(+) (comGC/cglC) [Streptococcus agalactiae strain GBS85147]
MKNLLLKCKDKKVKAFTLLEMLVVLIIISVLLLLFVPNLSKQKESVTRTGNAAVVKVVESQAELFELQETGRKASLSTLK
SGGYITEKQEKAYLDYYKDSSNGSQKISS

Nucleotide

• Length: 330 bp

>NTDB_id=136350 GBS85147_RS01000 WP_000793380.1 183773..184102(+) (comGC/cglC) [Streptococcus agalactiae strain GBS85147]
ATGAAAAATTTATTGTTAAAATGTAAGGATAAGAAGGTTAAAGCATTTACACTTTTAGAAATGTTAGTTGTTTTGATCAT
TATTTCGGTCTTGTTGTTATTGTTTGTACCCAATTTATCAAAACAAAAGGAAAGTGTCACAAGAACTGGAAATGCCGCTG
TTGTCAAGGTTGTAGAAAGTCAAGCCGAACTTTTTGAACTGCAAGAAACAGGTAGAAAAGCTAGTTTATCCACTCTAAAA
TCTGGAGGATATATTACTGAAAAGCAAGAAAAAGCCTATTTAGATTATTATAAAGACAGTTCCAATGGTTCTCAGAAAAT
TTCAAGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (11-34)

3D structure

• AlphaFold DB: A0A0E1EKJ1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGC/cglC	Streptococcus mitis NCTC 12261	60.55	100	0.606
comGC/cglC	Streptococcus mitis SK321	59.633	100	0.596
comYC	Streptococcus gordonii str. Challis substr. CH1	61.165	94.495	0.578
comGC/cglC	Streptococcus pneumoniae TIGR4	57.798	100	0.578
comGC/cglC	Streptococcus pneumoniae R6	57.798	100	0.578
comGC/cglC	Streptococcus pneumoniae Rx1	57.798	100	0.578
comGC/cglC	Streptococcus pneumoniae D39	57.798	100	0.578
comYC	Streptococcus mutans UA159	55.238	96.33	0.532
comYC	Streptococcus mutans UA140	55.238	96.33	0.532
comGC	Lactococcus lactis subsp. cremoris KW2	63.043	84.404	0.532
comYC	Streptococcus suis isolate S10	65.854	75.229	0.495