Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   GBS85147_RS00990 Genome accession   NZ_CP010319
Coordinates   181868..182839 (+) Length   323 a.a.
NCBI ID   WP_000250799.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain GBS85147     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 176868..187839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBS85147_RS00980 (GBS85147_0198) rpoC 177567..181217 (+) 3651 WP_000228729.1 DNA-directed RNA polymerase subunit beta' -
  GBS85147_RS00985 (GBS85147_0199) - 181331..181696 (+) 366 WP_000285373.1 DUF1033 family protein -
  GBS85147_RS00990 (GBS85147_0200) comYA 181868..182839 (+) 972 WP_000250799.1 competence type IV pilus ATPase ComGA Machinery gene
  GBS85147_RS00995 (GBS85147_0201) comYB 182733..183776 (+) 1044 WP_228322108.1 competence type IV pilus assembly protein ComGB Machinery gene
  GBS85147_RS01000 (GBS85147_0202) comGC/cglC 183773..184102 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  GBS85147_RS01005 (GBS85147_0203) comYD 184077..184490 (+) 414 WP_017646959.1 competence type IV pilus minor pilin ComGD Machinery gene
  GBS85147_RS01010 comGE 184462..184761 (+) 300 WP_025195478.1 competence type IV pilus minor pilin ComGE -
  GBS85147_RS01015 (GBS85147_0204) comGF 184715..185176 (+) 462 WP_001874060.1 competence type IV pilus minor pilin ComGF -
  GBS85147_RS01020 (GBS85147_0205) comGG 185154..185525 (+) 372 WP_000601103.1 competence type IV pilus minor pilin ComGG -
  GBS85147_RS01025 (GBS85147_0206) comYH 185640..186614 (+) 975 WP_001008568.1 class I SAM-dependent methyltransferase Machinery gene
  GBS85147_RS01030 (GBS85147_0207) - 186646..187839 (+) 1194 WP_000047537.1 acetate kinase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36832.29 Da        Isoelectric Point: 6.9167

>NTDB_id=136348 GBS85147_RS00990 WP_000250799.1 181868..182839(+) (comYA) [Streptococcus agalactiae strain GBS85147]
MVQSLAKHVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGTRGLYLFSGPVGSGKTTLMYQFASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSIPGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=136348 GBS85147_RS00990 WP_000250799.1 181868..182839(+) (comYA) [Streptococcus agalactiae strain GBS85147]
ATGGTTCAATCATTAGCAAAGCACGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTACAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTCGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCTCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTCCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAACTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

68.489

96.285

0.659
  comYA Streptococcus mutans UA140

68.489

96.285

0.659
  comYA Streptococcus gordonii str. Challis substr. CH1

63.323

98.762

0.625
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.379

96.285

0.601
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.415

96.285

0.591
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.415

96.285

0.591
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.415

96.285

0.591
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.415

96.285

0.591
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.613

95.975

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

50.311

99.69

0.502

Multiple sequence alignment