Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   GBS85147_RS00995 Genome accession   NZ_CP010319
Coordinates   182733..183776 (+) Length   347 a.a.
NCBI ID   WP_228322108.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain GBS85147     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 177733..188776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBS85147_RS00985 (GBS85147_0199) - 181331..181696 (+) 366 WP_000285373.1 DUF1033 family protein -
  GBS85147_RS00990 (GBS85147_0200) comYA 181868..182839 (+) 972 WP_000250799.1 competence type IV pilus ATPase ComGA Machinery gene
  GBS85147_RS00995 (GBS85147_0201) comYB 182733..183776 (+) 1044 WP_228322108.1 competence type IV pilus assembly protein ComGB Machinery gene
  GBS85147_RS01000 (GBS85147_0202) comGC/cglC 183773..184102 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  GBS85147_RS01005 (GBS85147_0203) comYD 184077..184490 (+) 414 WP_017646959.1 competence type IV pilus minor pilin ComGD Machinery gene
  GBS85147_RS01010 comGE 184462..184761 (+) 300 WP_025195478.1 competence type IV pilus minor pilin ComGE -
  GBS85147_RS01015 (GBS85147_0204) comGF 184715..185176 (+) 462 WP_001874060.1 competence type IV pilus minor pilin ComGF -
  GBS85147_RS01020 (GBS85147_0205) comGG 185154..185525 (+) 372 WP_000601103.1 competence type IV pilus minor pilin ComGG -
  GBS85147_RS01025 (GBS85147_0206) comYH 185640..186614 (+) 975 WP_001008568.1 class I SAM-dependent methyltransferase Machinery gene
  GBS85147_RS01030 (GBS85147_0207) - 186646..187839 (+) 1194 WP_000047537.1 acetate kinase -
  GBS85147_RS01035 (GBS85147_0208) - 187991..188197 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  GBS85147_RS09950 - 188256..188393 (+) 138 WP_079263416.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39961.70 Da        Isoelectric Point: 9.9642

>NTDB_id=136349 GBS85147_RS00995 WP_228322108.1 182733..183776(+) (comYB) [Streptococcus agalactiae strain GBS85147]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVAAYPLILLSFLVLIMIGLRNYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=136349 GBS85147_RS00995 WP_228322108.1 182733..183776(+) (comYB) [Streptococcus agalactiae strain GBS85147]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGGATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTGCTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGAATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTGTTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGGTATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGTCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(218-338)

Type II secretion system (T2SS), protein F (T2SSF)

(33-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

62.17

98.271

0.611
  comYB Streptococcus mutans UA159

61.877

98.271

0.608
  comYB Streptococcus gordonii str. Challis substr. CH1

56.012

98.271

0.55
  comGB/cglB Streptococcus mitis NCTC 12261

54.925

96.542

0.53
  comGB/cglB Streptococcus mitis SK321

54.328

96.542

0.524
  comGB/cglB Streptococcus pneumoniae Rx1

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae D39

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae R6

53.433

96.542

0.516
  comGB/cglB Streptococcus pneumoniae TIGR4

53.433

96.542

0.516
  comGB Lactococcus lactis subsp. cremoris KW2

50.148

97.118

0.487

Multiple sequence alignment