Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LG36_RS10805 Genome accession   NZ_CP009472
Coordinates   2229030..2229968 (-) Length   312 a.a.
NCBI ID   WP_038602362.1    Uniprot ID   -
Organism   Lactococcus lactis strain AI06     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2224030..2234968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG36_RS10765 (LG36_2056) - 2224918..2225760 (-) 843 WP_038602352.1 metal ABC transporter substrate-binding protein -
  LG36_RS10770 (LG36_2057) - 2225757..2226194 (-) 438 WP_021721874.1 zinc-dependent MarR family transcriptional regulator -
  LG36_RS10775 (LG36_2058) comGG 2226275..2226550 (-) 276 WP_021721873.1 hypothetical protein Machinery gene
  LG36_RS10780 (LG36_2059) comGF 2226598..2227044 (-) 447 WP_021721872.1 competence type IV pilus minor pilin ComGF Machinery gene
  LG36_RS10785 (LG36_2060) comGE 2227007..2227303 (-) 297 WP_038602356.1 competence type IV pilus minor pilin ComGE Machinery gene
  LG36_RS10790 (LG36_2061) comGD 2227275..2227706 (-) 432 WP_080651959.1 competence type IV pilus minor pilin ComGD Machinery gene
  LG36_RS10795 (LG36_2062) comGC 2227666..2228049 (-) 384 WP_080739889.1 competence type IV pilus major pilin ComGC Machinery gene
  LG36_RS10800 (LG36_2063) comGB 2228063..2229136 (-) 1074 WP_080739890.1 competence type IV pilus assembly protein ComGB Machinery gene
  LG36_RS10805 (LG36_2064) comGA 2229030..2229968 (-) 939 WP_038602362.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35592.88 Da        Isoelectric Point: 6.6176

>NTDB_id=129665 LG36_RS10805 WP_038602362.1 2229030..2229968(-) (comGA) [Lactococcus lactis strain AI06]
MIPKMAKELIQKAIDRGASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKELNFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFELARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHAASVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTSTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=129665 LG36_RS10805 WP_038602362.1 2229030..2229968(-) (comGA) [Lactococcus lactis strain AI06]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAAAGCAATTGATAGAGGTGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTACGTCTTTCAACAGTGGGGGATTTCGAGGGTAATGAATCCTTGGTAATACGTCT
CTTGCATGATCAAAACAAAGAACTTAATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCTGGTCCAGTAGGATCGGGCAAAACATCTTTAATGTTTGAGTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCTGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTATTTTCAACCGTTCATGCCGCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGATATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACATCTACTGAAGCCAAGAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

77.564

100

0.776
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

55.305

99.679

0.551
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

54.341

99.679

0.542
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.376

99.679

0.532
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.603

100

0.516

Multiple sequence alignment