Detailed information NTG0129660

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LG36_RS10780
• Genome accession: NZ_CP009472
• Coordinates: 2226598..2227044 (-)
• Length: 148 a.a.
• NCBI ID: WP_021721872.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain AI06
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2221598..2232044

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LG36_RS10745 (LG36_2052)	-	2221716..2222093 (-)	378	WP_038602343.1	pyridoxamine 5'-phosphate oxidase family protein	-
LG36_RS10750 (LG36_2053)	-	2222298..2223164 (+)	867	WP_038602346.1	RluA family pseudouridine synthase	-
LG36_RS10755 (LG36_2054)	-	2223202..2224011 (-)	810	WP_038602349.1	metal ABC transporter permease	-
LG36_RS10760 (LG36_2055)	-	2224004..2224741 (-)	738	WP_021723055.1	metal ABC transporter ATP-binding protein	-
LG36_RS10765 (LG36_2056)	-	2224918..2225760 (-)	843	WP_038602352.1	metal ABC transporter substrate-binding protein	-
LG36_RS10770 (LG36_2057)	-	2225757..2226194 (-)	438	WP_021721874.1	zinc-dependent MarR family transcriptional regulator	-
LG36_RS10775 (LG36_2058)	comGG	2226275..2226550 (-)	276	WP_021721873.1	hypothetical protein	Machinery gene
LG36_RS10780 (LG36_2059)	comGF	2226598..2227044 (-)	447	WP_021721872.1	competence type IV pilus minor pilin ComGF	Machinery gene
LG36_RS10785 (LG36_2060)	comGE	2227007..2227303 (-)	297	WP_038602356.1	competence type IV pilus minor pilin ComGE	Machinery gene
LG36_RS10790 (LG36_2061)	comGD	2227275..2227706 (-)	432	WP_080651959.1	competence type IV pilus minor pilin ComGD	Machinery gene
LG36_RS10795 (LG36_2062)	comGC	2227666..2228049 (-)	384	WP_080739889.1	competence type IV pilus major pilin ComGC	Machinery gene
LG36_RS10800 (LG36_2063)	comGB	2228063..2229136 (-)	1074	WP_080739890.1	competence type IV pilus assembly protein ComGB	Machinery gene
LG36_RS10805 (LG36_2064)	comGA	2229030..2229968 (-)	939	WP_038602362.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17156.02 Da
• Isoelectric Point: 9.0730

>NTDB_id=129660 LG36_RS10780 WP_021721872.1 2226598..2227044(-) (comGF) [Lactococcus lactis strain AI06]
MERKFCDLNLKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQMAISQSDSIKDWQIFCQQMRFELSGTKLDKVEQNFL
YVTKDKKLRFGFMNDDFRKTDDKGQGYQPMLYDIKGAKIQATKNLITIRIDFNQGGERTFIYQFPEDK

Nucleotide

• Length: 447 bp

>NTDB_id=129660 LG36_RS10780 WP_021721872.1 2226598..2227044(-) (comGF) [Lactococcus lactis strain AI06]
ATGGAAAGGAAATTTTGCGACTTGAACCTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGATCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAATGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAAGGTAGAACAGAATTTTCTA
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAATGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGGGGCTAAGATTCAAGCTACTAAAAATCTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATCTATCAATTTCCAGAAGACAAATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	78.014	95.27	0.743
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	42.143	94.595	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.714	94.595	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.714	94.595	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.714	94.595	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.714	94.595	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	40.714	94.595	0.385