Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IX91_RS02210 Genome accession   NZ_CP009354
Coordinates   439963..440388 (-) Length   141 a.a.
NCBI ID   WP_004742603.1    Uniprot ID   F9SZQ1
Organism   Vibrio tubiashii ATCC 19109     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434963..445388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IX91_RS02190 (IX91_02245) coaE 435494..436102 (-) 609 WP_004742599.1 dephospho-CoA kinase -
  IX91_RS02195 (IX91_02250) pilD 436104..436973 (-) 870 WP_004742600.1 A24 family peptidase Machinery gene
  IX91_RS02200 (IX91_02255) pilC 437032..438255 (-) 1224 WP_004742601.1 type II secretion system F family protein Machinery gene
  IX91_RS02205 (IX91_02260) pilB 438266..439957 (-) 1692 WP_004742602.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IX91_RS02210 (IX91_02265) pilA 439963..440388 (-) 426 WP_004742603.1 pilin Machinery gene
  IX91_RS02215 (IX91_02270) nadC 440632..441519 (-) 888 WP_004742604.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IX91_RS02220 (IX91_02275) ampD 441651..442205 (+) 555 WP_004742605.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IX91_RS02225 (IX91_02280) fldB 442409..442930 (-) 522 WP_004742606.1 flavodoxin FldB -
  IX91_RS02230 (IX91_02285) xerD 443094..443990 (+) 897 WP_004742607.1 site-specific tyrosine recombinase XerD -
  IX91_RS02235 (IX91_02290) - 444031..444783 (+) 753 WP_004742608.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14646.63 Da        Isoelectric Point: 6.9455

>NTDB_id=128850 IX91_RS02210 WP_004742603.1 439963..440388(-) (pilA) [Vibrio tubiashii ATCC 19109]
MQSLQSRKTKGFTLIELMIVVAVIGVLAAIAVPQYQTYVAKSEASSALASITGHRANVETYVLTEGSFPANSSALAIPSS
PLGIISYDNVSSAAGDILFTFSSSGVSPDVVSKKVSLKRDGNGAWTCQSDVDSDLKPKGCS

Nucleotide


Download         Length: 426 bp        

>NTDB_id=128850 IX91_RS02210 WP_004742603.1 439963..440388(-) (pilA) [Vibrio tubiashii ATCC 19109]
ATGCAAAGCTTACAAAGCCGTAAAACCAAAGGCTTTACCTTAATTGAGTTGATGATTGTGGTTGCCGTTATTGGCGTTCT
AGCAGCAATTGCGGTGCCTCAATATCAAACATATGTAGCGAAAAGCGAGGCATCCTCAGCATTAGCCTCAATAACCGGAC
ACCGTGCCAATGTCGAAACGTATGTTCTGACAGAAGGGAGCTTTCCTGCGAACTCATCTGCCTTGGCAATACCTTCCTCA
CCTCTTGGCATCATTAGCTATGACAATGTTTCTTCAGCGGCGGGAGATATTCTCTTCACTTTCAGCTCTTCAGGCGTTAG
TCCTGATGTTGTTAGTAAAAAAGTCTCTCTAAAGCGTGATGGAAACGGGGCTTGGACTTGCCAGTCTGACGTAGACAGCG
ACCTAAAGCCAAAAGGCTGTTCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F9SZQ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.02

100

0.504
  pilA Vibrio cholerae strain A1552

47.02

100

0.504
  pilA Vibrio cholerae C6706

47.02

100

0.504
  pilA Pseudomonas aeruginosa PAK

42.069

100

0.433
  pilA Acinetobacter baumannii strain A118

45.185

95.745

0.433
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.278

100

0.411
  pilA/pilAII Pseudomonas stutzeri DSM 10701

41.606

97.163

0.404
  pilA Acinetobacter nosocomialis M2

38.732

100

0.39
  pilE Neisseria gonorrhoeae MS11

38.636

93.617

0.362

Multiple sequence alignment