Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IX91_RS02205 Genome accession   NZ_CP009354
Coordinates   438266..439957 (-) Length   563 a.a.
NCBI ID   WP_004742602.1    Uniprot ID   -
Organism   Vibrio tubiashii ATCC 19109     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433266..444957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IX91_RS02175 (IX91_02225) rplS 433668..434021 (+) 354 WP_004742596.1 50S ribosomal protein L19 -
  IX91_RS02180 (IX91_02235) yacG 434462..434656 (-) 195 WP_004742597.1 DNA gyrase inhibitor YacG -
  IX91_RS02185 (IX91_02240) zapD 434719..435459 (-) 741 WP_004742598.1 cell division protein ZapD -
  IX91_RS02190 (IX91_02245) coaE 435494..436102 (-) 609 WP_004742599.1 dephospho-CoA kinase -
  IX91_RS02195 (IX91_02250) pilD 436104..436973 (-) 870 WP_004742600.1 A24 family peptidase Machinery gene
  IX91_RS02200 (IX91_02255) pilC 437032..438255 (-) 1224 WP_004742601.1 type II secretion system F family protein Machinery gene
  IX91_RS02205 (IX91_02260) pilB 438266..439957 (-) 1692 WP_004742602.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IX91_RS02210 (IX91_02265) pilA 439963..440388 (-) 426 WP_004742603.1 pilin Machinery gene
  IX91_RS02215 (IX91_02270) nadC 440632..441519 (-) 888 WP_004742604.1 carboxylating nicotinate-nucleotide diphosphorylase -
  IX91_RS02220 (IX91_02275) ampD 441651..442205 (+) 555 WP_004742605.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IX91_RS02225 (IX91_02280) fldB 442409..442930 (-) 522 WP_004742606.1 flavodoxin FldB -
  IX91_RS02230 (IX91_02285) xerD 443094..443990 (+) 897 WP_004742607.1 site-specific tyrosine recombinase XerD -
  IX91_RS02235 (IX91_02290) - 444031..444783 (+) 753 WP_004742608.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62452.58 Da        Isoelectric Point: 5.5119

>NTDB_id=128849 IX91_RS02205 WP_004742602.1 438266..439957(-) (pilB) [Vibrio tubiashii ATCC 19109]
MLTNLPSILRHAELLDPAQEAQLVELVNDTSLTVAEVIIEQGWLTQAELVSQLSRIFALPIIDFANYDYQAVCHSLGLRE
LITRSRAVPIRQSEHQLLIAVTDPTNIAIEEEFRFATGKQIELVLADVKQLNGALRRLYGSAICGEEASHAKELSEQELV
SLVEISNDEMNPVEDLSSDNSPVSRYINQILLDAVRKKASDIHFEPYEASYRVRFRCDGILIETQQPSHTLSRRLSARLK
IIAKLDIAERRLPQDGRIKLQLSASTAVDIRISTLPTLWGEKIVLRLLDSSSAELDIQSLGYNQHQQALYQKLLSKPQGM
ILMTGPTGSGKTVSLYAGLNMLNTPQVNIATAEDPVEINLPGVNQVQINPRIGLDFVQALRAFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAESIVRLQHMGISPFNLSSSLSLIIAQRLARKLCNHCKQQQTLPEAHCQQLELANN
TVVFAASEQGCSECNQGYFGRTGIYEVMQIDDKLVQVINQKADAKAIEKIAIKQGMQTLKESGTEKLLAGVTSYQELQRV
LYL

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=128849 IX91_RS02205 WP_004742602.1 438266..439957(-) (pilB) [Vibrio tubiashii ATCC 19109]
ATGCTAACCAACCTACCCTCTATTTTACGCCATGCTGAGCTTCTTGATCCTGCTCAAGAAGCTCAGTTAGTTGAACTGGT
TAACGATACAAGCCTTACCGTGGCCGAAGTCATAATAGAGCAAGGTTGGCTCACCCAAGCAGAGCTAGTCTCACAACTCT
CGCGAATTTTTGCCTTACCCATCATCGACTTTGCTAATTACGACTATCAAGCGGTATGCCACAGTTTAGGCCTGCGCGAA
CTGATCACCCGCTCCCGAGCTGTACCTATTAGGCAGAGTGAACACCAACTGCTTATCGCCGTTACCGACCCGACCAATAT
CGCAATCGAAGAAGAGTTTCGCTTTGCGACCGGAAAGCAGATTGAGTTAGTTTTAGCTGACGTAAAGCAACTCAATGGCG
CGTTACGTCGCTTGTATGGCAGCGCTATCTGTGGTGAAGAAGCGAGTCATGCAAAAGAGCTGAGCGAACAAGAGCTCGTC
AGCTTAGTCGAGATCTCTAATGATGAGATGAACCCAGTCGAAGACCTGAGTAGTGATAACTCCCCAGTCAGTCGGTATAT
CAATCAAATCTTATTGGATGCGGTCAGGAAAAAGGCATCTGATATTCACTTTGAACCCTATGAAGCCTCTTATCGTGTGC
GCTTTCGCTGTGATGGAATTTTGATTGAGACTCAACAACCTAGCCATACATTAAGCCGTCGGTTATCCGCAAGACTAAAG
ATCATCGCCAAACTCGATATTGCCGAGCGCCGCTTACCTCAAGATGGGCGCATCAAGCTTCAACTCAGTGCAAGTACCGC
TGTTGATATTCGAATTTCAACCTTACCAACCCTGTGGGGCGAGAAGATCGTGCTACGCCTTCTCGATAGCAGCAGTGCCG
AGTTAGACATTCAATCACTAGGGTACAACCAACACCAACAAGCTTTGTATCAAAAATTACTGAGTAAACCCCAAGGCATG
ATTTTGATGACAGGACCAACGGGCAGCGGTAAGACAGTCTCTCTTTATGCGGGGTTAAACATGCTCAATACCCCACAAGT
GAACATTGCAACCGCAGAAGACCCGGTGGAAATTAATTTACCGGGCGTTAACCAAGTTCAAATCAACCCCAGAATTGGAC
TAGACTTCGTCCAAGCGTTGCGCGCCTTTTTACGCCAAGACCCGGATATTGTCATGGTCGGTGAGATACGCGACCTTGAA
ACGGCTGAAATCGCAGTCAAAGCGGCACAAACCGGTCACCTAGTGCTATCCACATTGCATACAAACTCGGCTGCCGAAAG
CATCGTTCGCTTGCAACATATGGGCATCTCCCCATTTAATCTATCTTCATCACTGAGCTTAATCATTGCCCAACGTTTGG
CACGAAAGCTGTGTAACCATTGCAAGCAGCAGCAAACCTTGCCCGAAGCGCACTGCCAACAACTGGAGTTAGCAAACAAC
ACCGTGGTTTTTGCTGCTAGTGAGCAAGGGTGCAGTGAATGCAATCAAGGCTACTTTGGGCGAACCGGAATCTATGAAGT
GATGCAGATAGACGACAAGTTAGTGCAAGTCATCAATCAAAAGGCGGATGCTAAAGCCATCGAAAAGATCGCGATAAAGC
AAGGAATGCAGACGCTTAAAGAGTCTGGAACGGAGAAACTGCTCGCAGGAGTCACCAGTTACCAAGAGTTGCAACGTGTT
CTTTACTTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

63.83

100

0.639
  pilB Vibrio parahaemolyticus RIMD 2210633

62.014

100

0.623
  pilB Vibrio campbellii strain DS40M4

61.634

100

0.616
  pilB Acinetobacter baumannii D1279779

48.127

94.849

0.456
  pilB Legionella pneumophila strain ERS1305867

45.699

99.112

0.453
  pilB Acinetobacter baylyi ADP1

47.985

92.54

0.444
  pilF Neisseria gonorrhoeae MS11

43.985

94.494

0.416
  pilB Haemophilus influenzae Rd KW20

43.299

86.146

0.373
  pilB Haemophilus influenzae 86-028NP

42.827

85.435

0.366
  pilB/pilB1 Synechocystis sp. PCC 6803

38.815

92.895

0.361

Multiple sequence alignment