Detailed information NTG0128847

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: IX91_RS02195
• Genome accession: NZ_CP009354
• Coordinates: 436104..436973 (-)
• Length: 289 a.a.
• NCBI ID: WP_004742600.1
• Uniprot ID: F9SZP8
• Organism: Vibrio tubiashii ATCC 19109
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 431104..441973

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IX91_RS02160 (IX91_02210)	rpsP	432029..432277 (+)	249	WP_004742593.1	30S ribosomal protein S16	-
IX91_RS02165 (IX91_02215)	rimM	432307..432855 (+)	549	WP_004742594.1	ribosome maturation factor RimM	-
IX91_RS02170 (IX91_02220)	trmD	432883..433626 (+)	744	WP_004742595.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
IX91_RS02175 (IX91_02225)	rplS	433668..434021 (+)	354	WP_004742596.1	50S ribosomal protein L19	-
IX91_RS02180 (IX91_02235)	yacG	434462..434656 (-)	195	WP_004742597.1	DNA gyrase inhibitor YacG	-
IX91_RS02185 (IX91_02240)	zapD	434719..435459 (-)	741	WP_004742598.1	cell division protein ZapD	-
IX91_RS02190 (IX91_02245)	coaE	435494..436102 (-)	609	WP_004742599.1	dephospho-CoA kinase	-
IX91_RS02195 (IX91_02250)	pilD	436104..436973 (-)	870	WP_004742600.1	A24 family peptidase	Machinery gene
IX91_RS02200 (IX91_02255)	pilC	437032..438255 (-)	1224	WP_004742601.1	type II secretion system F family protein	Machinery gene
IX91_RS02205 (IX91_02260)	pilB	438266..439957 (-)	1692	WP_004742602.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IX91_RS02210 (IX91_02265)	pilA	439963..440388 (-)	426	WP_004742603.1	pilin	Machinery gene
IX91_RS02215 (IX91_02270)	nadC	440632..441519 (-)	888	WP_004742604.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32357.41 Da
• Isoelectric Point: 4.9261

>NTDB_id=128847 IX91_RS02195 WP_004742600.1 436104..436973(-) (pilD) [Vibrio tubiashii ATCC 19109]
MDVFDYYPWLFPTLATLFGLIVGSFLNVVIHRLPIIMEREWRAECAESFPEYQISPPEGTYNLSVPNSTCPKCDTPIRMI
DNIPVVSWLILKGKCRQCDNKISMRYPLIEILSAGLCFVIANQFALNYFSIALLFFTFTLIAATFIDLDTMLLPDQLTLP
LTWAGIALALFGISPVSLQDAVIGAMAGYLALWSVYWLFKLATGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSLVGL
VFGLIQLKLKSQGIDKAFPFGPYLAIAGWLSMMWGNNILNWYLSNFLGI

Nucleotide

• Length: 870 bp

>NTDB_id=128847 IX91_RS02195 WP_004742600.1 436104..436973(-) (pilD) [Vibrio tubiashii ATCC 19109]
ATGGACGTTTTTGACTATTACCCTTGGCTTTTTCCTACACTAGCAACATTGTTTGGTCTTATCGTAGGCAGCTTTTTAAA
CGTGGTTATTCATCGCCTGCCGATCATCATGGAACGTGAATGGCGAGCCGAATGTGCGGAAAGCTTTCCTGAATACCAAA
TATCGCCCCCAGAAGGGACTTACAACCTCAGCGTTCCAAATTCAACTTGCCCCAAATGTGATACCCCAATTCGCATGATT
GACAACATTCCTGTTGTTAGTTGGCTGATACTGAAAGGGAAATGTCGTCAATGCGATAATAAAATAAGCATGCGCTACCC
TCTGATTGAGATCTTATCAGCAGGTCTATGCTTTGTTATCGCTAATCAGTTTGCATTGAACTACTTCTCGATTGCCTTGC
TGTTTTTCACCTTTACTCTTATCGCGGCAACCTTTATTGATCTCGACACCATGCTGCTGCCAGATCAACTCACTCTTCCT
CTAACCTGGGCGGGAATTGCGCTCGCACTGTTTGGGATTAGCCCAGTATCTCTACAAGACGCGGTGATCGGTGCAATGGC
TGGCTATCTTGCTTTATGGTCAGTTTACTGGCTGTTTAAGCTAGCCACAGGCAAAGAAGGAATGGGTTACGGAGACTTTA
AACTGCTTGCCGCCTTGGGCGCTTGGCTTGGTTGGCAATATTTGCCGATGATAATTTTGCTCTCTTCTTTAGTTGGCTTA
GTTTTTGGCCTCATTCAGCTCAAATTGAAAAGCCAAGGAATCGACAAAGCCTTCCCCTTCGGTCCTTATTTAGCTATTGC
AGGTTGGTTGAGCATGATGTGGGGAAATAACATTCTCAATTGGTATTTAAGTAACTTTTTAGGAATCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-123)

3D structure

• AlphaFold DB: F9SZP8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	74.048	100	0.74
pilD	Vibrio cholerae strain A1552	71.528	99.654	0.713
pilD	Acinetobacter baumannii D1279779	49.077	93.772	0.46
pilD	Acinetobacter nosocomialis M2	49.077	93.772	0.46
pilD	Neisseria gonorrhoeae MS11	47.273	95.156	0.45