Detailed information NTG1170550

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACMTA2_RS12310
• Genome accession: NZ_OZ221631
• Coordinates: 2768367..2769482 (+)
• Length: 371 a.a.
• NCBI ID: WP_413795063.1
• Uniprot ID: -
• Organism: Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2763367..2774482

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMTA2_RS12295	-	2764334..2765248 (+)	915	WP_413795061.1	sugar kinase	-
ACMTA2_RS12300	-	2765373..2765648 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
ACMTA2_RS12305	-	2765697..2768234 (-)	2538	WP_413795062.1	PAS domain-containing protein	-
ACMTA2_RS12310	pilU	2768367..2769482 (+)	1116	WP_413795063.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACMTA2_RS12315	-	2769600..2771555 (-)	1956	WP_413795064.1	acetoacetate--CoA ligase	-
ACMTA2_RS12320	-	2771654..2772430 (-)	777	WP_413795065.1	3-hydroxybutyrate dehydrogenase	-
ACMTA2_RS12325	-	2772438..2773829 (-)	1392	WP_413795066.1	GntP family permease	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41234.13 Da
• Isoelectric Point: 6.0675

>NTDB_id=1170550 ACMTA2_RS12310 WP_413795063.1 2768367..2769482(+) (pilU) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVLKPLGNEAFKVGDIARLAESLMDAEQRVEFDRELEMNLAISLAGVGR
FRVNVFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDSVMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLANNLQAFVSQRLVRTRSGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FSLVVEGAIDEEEALKNADSANNLRLRLKLHSESGAVPPADPAAGEWGLMD

Nucleotide

• Length: 1116 bp

>NTDB_id=1170550 ACMTA2_RS12310 WP_413795063.1 2768367..2769482(+) (pilU) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTTTACATGTCCACGGGGGCGCCGCCGTG
TGCGCGTTTCGAGGGCGTGCTCAAGCCCTTGGGCAACGAGGCTTTCAAGGTCGGCGACATTGCCAGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCGTCGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATCTCCCTGGCCGGTGTCGGACGG
TTCCGGGTCAATGTCTTCAAACAGCGCAATGACGTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCACGCTTCGA
AGACCTGAAACTGCCCCGGGTGCTGTTGGACAGCGTCATGCAGAAACAGGGGTTGATGCTGTTTGTCGGTGCGACGGGCT
CGGGCAAGTCGACTTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAACCAACGGGAAGTCGGTGTCGACACCCGCAGTTTCCATGC
GGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCATG
CCCTGGCTTTCGCTGACACCGGGCACCTGGTGATTTCCACCTTGCATGCCCATAACGCCAACCAGGCCCTGGATCGGGTA
ATCAATTTCTTTCCGGAAGAACGCCGACCGCAACTGCTCAACGACCTTGCCAACAACCTCCAGGCATTCGTTTCCCAGCG
CCTGGTGCGCACCCGCTCCGGCCAGCGCCGGGCGGCGGTGGAGGTGATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GGCGCAACGAACTGGGCGAGCTCAAGGGCATCATGGAGAAGTCAGAGGAGTTGGGGATGCAAACCTTTGACCAGGCCTTG
TTCAGCCTGGTGGTCGAAGGCGCCATCGATGAGGAAGAAGCGCTCAAAAATGCCGACTCGGCGAACAACCTGCGGTTGCG
GCTGAAGTTGCATAGCGAGTCGGGCGCGGTGCCACCGGCCGATCCGGCGGCGGGAGAGTGGGGGCTTATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	95.687	0.539
pilU	Vibrio cholerae strain A1552	52.857	94.34	0.499
pilU	Acinetobacter baylyi ADP1	51.685	95.957	0.496
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.459	99.73	0.394
pilT	Vibrio cholerae strain A1552	41.141	89.757	0.369
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.757	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.722	0.367
pilT	Acinetobacter nosocomialis M2	40.419	90.027	0.364
pilT	Acinetobacter baumannii D1279779	40.419	90.027	0.364
pilT	Acinetobacter baumannii strain A118	40.419	90.027	0.364
pilT	Pseudomonas aeruginosa PAK	39.244	92.722	0.364