Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ACLV7Z_RS01165 Genome accession   NZ_OZ217343
Coordinates   222419..223360 (+) Length   313 a.a.
NCBI ID   WP_218774872.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis G22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 217419..228360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Z_RS01145 (SMIG22_02240) - 217485..219302 (+) 1818 WP_218774870.1 acyltransferase family protein -
  ACLV7Z_RS01150 (SMIG22_02250) nagA 219454..220605 (+) 1152 WP_218774871.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7Z_RS01155 (SMIG22_02260) - 220768..221826 (+) 1059 WP_004257473.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7Z_RS01160 (SMIG22_02270) - 221977..222342 (+) 366 WP_000286413.1 DUF1033 family protein -
  ACLV7Z_RS01165 (SMIG22_02280) comGA/cglA/cilD 222419..223360 (+) 942 WP_218774872.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7Z_RS01170 (SMIG22_02290) comGB/cglB 223308..224324 (+) 1017 WP_218774887.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7Z_RS01175 (SMIG22_02300) comGC/cglC 224326..224649 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  ACLV7Z_RS01180 (SMIG22_02310) comGD/cglD 224612..225046 (+) 435 WP_033628889.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7Z_RS01185 (SMIG22_02320) comGE/cglE 225009..225311 (+) 303 WP_071478895.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7Z_RS01190 (SMIG22_02330) comGF/cglF 225274..225735 (+) 462 WP_218774873.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7Z_RS01195 (SMIG22_02340) comGG/cglG 225713..226126 (+) 414 WP_218774874.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7Z_RS01200 (SMIG22_02350) - 226159..226746 (+) 588 WP_218774875.1 class I SAM-dependent methyltransferase -
  ACLV7Z_RS01205 (SMIG22_02360) comYH 226807..227760 (+) 954 WP_218774876.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35550.52 Da        Isoelectric Point: 5.8494

>NTDB_id=1170167 ACLV7Z_RS01165 WP_218774872.1 222419..223360(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis G22]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRIGDERCKIGCYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDDKMASLRLSTVGDYRGYESLVIRLLHDEKQDLHFWFQDIDELGKQYRQRGLYLFAGPVGSGKTTLMHELAKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANKDYQEHQSTSWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1170167 ACLV7Z_RS01165 WP_218774872.1 222419..223360(+) (comGA/cglA/cilD) [Streptococcus mitis isolate S. mitis G22]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGAGCGCAAGATATTTATTTTGTTCCTAAGTT
AGACGCTTATGAGCTTCATATGAGAATAGGAGACGAGCGCTGTAAAATCGGTTGTTATGATTTTGAAAAGTTTGCGGCCG
TCATCAGTCACTTTAAGTTTGTAGCTGGTATGAATGTTGGAGAAAAGCGACGTAGTCAGCTTGGTTCCTGCGACTATGCC
TATGATGATAAGATGGCGTCCCTACGTCTATCTACCGTAGGCGATTATCGAGGATATGAGAGTTTAGTAATTCGCTTGTT
GCATGATGAGAAGCAGGATTTACATTTTTGGTTTCAGGATATCGACGAATTGGGCAAGCAGTACAGGCAACGGGGACTTT
ATCTTTTTGCTGGTCCAGTCGGAAGTGGCAAGACAACCCTGATGCACGAATTGGCCAAGTCCCTCTTTAAAGGACAGCAA
GTCATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGGTTGAC
CTATGAAAATCTAATCAAACTTTCTTTGCGGCATCGTCCTGATCTCTTGATTATCGGAGAAATTCGGGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCCAGTTTGACAGGGGCGACAGTCTTTTCAACCATTCATGCCAAGAGTATTCGAGGTGTTTAT
GAGCGCCTGCTGGAGTTGGGTGTGAGTGAGGAAGAGTTGGCAGTCGTTCTGCAAGGTGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAAAGACTATCAAGAACACCAGTCAACTAGCTGGAATGAACAGATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.444

100

0.974
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

95.527

100

0.955
  comGA/cglA/cilD Streptococcus pneumoniae D39

95.527

100

0.955
  comGA/cglA/cilD Streptococcus pneumoniae R6

95.527

100

0.955
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

95.527

100

0.955
  comYA Streptococcus gordonii str. Challis substr. CH1

79.355

99.042

0.786
  comYA Streptococcus mutans UA159

66.559

99.361

0.661
  comYA Streptococcus mutans UA140

66.559

99.361

0.661
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.449

99.681

0.553
  comGA Latilactobacillus sakei subsp. sakei 23K

42.647

86.901

0.371

Multiple sequence alignment