Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE/cglE   Type   Machinery gene
Locus tag   ACLV7Z_RS01185 Genome accession   NZ_OZ217343
Coordinates   225009..225311 (+) Length   100 a.a.
NCBI ID   WP_071478895.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis G22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 220009..230311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Z_RS01155 (SMIG22_02260) - 220768..221826 (+) 1059 WP_004257473.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7Z_RS01160 (SMIG22_02270) - 221977..222342 (+) 366 WP_000286413.1 DUF1033 family protein -
  ACLV7Z_RS01165 (SMIG22_02280) comGA/cglA/cilD 222419..223360 (+) 942 WP_218774872.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7Z_RS01170 (SMIG22_02290) comGB/cglB 223308..224324 (+) 1017 WP_218774887.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7Z_RS01175 (SMIG22_02300) comGC/cglC 224326..224649 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  ACLV7Z_RS01180 (SMIG22_02310) comGD/cglD 224612..225046 (+) 435 WP_033628889.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7Z_RS01185 (SMIG22_02320) comGE/cglE 225009..225311 (+) 303 WP_071478895.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7Z_RS01190 (SMIG22_02330) comGF/cglF 225274..225735 (+) 462 WP_218774873.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7Z_RS01195 (SMIG22_02340) comGG/cglG 225713..226126 (+) 414 WP_218774874.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7Z_RS01200 (SMIG22_02350) - 226159..226746 (+) 588 WP_218774875.1 class I SAM-dependent methyltransferase -
  ACLV7Z_RS01205 (SMIG22_02360) comYH 226807..227760 (+) 954 WP_218774876.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV7Z_RS01210 (SMIG22_02370) - 227810..229000 (+) 1191 WP_173306883.1 acetate kinase -
  ACLV7Z_RS01215 (SMIG22_02380) rnpA 229148..229519 (+) 372 WP_000739243.1 ribonuclease P protein component -

Sequence

Protein


Download         Length: 100 a.a.        Molecular weight: 11121.08 Da        Isoelectric Point: 9.7273

>NTDB_id=1170171 ACLV7Z_RS01185 WP_071478895.1 225009..225311(+) (comGE/cglE) [Streptococcus mitis isolate S. mitis G22]
MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVVRVAKMALQTGQNQVNINGVEIQVFS
SEKGLEVYHGSEQLLAIKEP

Nucleotide


Download         Length: 303 bp        

>NTDB_id=1170171 ACLV7Z_RS01185 WP_071478895.1 225009..225311(+) (comGE/cglE) [Streptococcus mitis isolate S. mitis G22]
ATGGAAAAATTAAACGCATTAAGGAAACAAAAAATTAGGGCAGTGATTTTACTGGAAGCAGTAGTCGCTCTAGCTATTTT
TGCTAGCATTGCGACCCTCCTTTTGGGACAAATTCAAAAAAATAGGCAAGAAGAAGCAAAAATCTTGCAGAAAGAAGAAG
TTGTGCGTGTCGCTAAGATGGCTCTGCAGACAGGTCAAAATCAGGTAAACATAAACGGAGTGGAGATTCAGGTGTTTTCT
AGTGAAAAGGGATTGGAGGTTTATCATGGTTCAGAACAGTTGTTGGCTATCAAAGAGCCATAA

Domains

Predicted by InterproScan.

Type II secretory pathway pseudopilin (ComGE)

(16-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE/cglE Streptococcus mitis NCTC 12261

98

100

0.98
  comGE/cglE Streptococcus pneumoniae Rx1

98

100

0.98
  comGE/cglE Streptococcus pneumoniae D39

98

100

0.98
  comGE/cglE Streptococcus pneumoniae R6

98

100

0.98
  comGE/cglE Streptococcus pneumoniae TIGR4

98

100

0.98
  comGE/cglE Streptococcus mitis SK321

97

100

0.97
  comYE Streptococcus mutans UA140

42.222

90

0.38
  comYE Streptococcus mutans UA159

42.222

90

0.38

Multiple sequence alignment