Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ACLV7Z_RS01170 Genome accession   NZ_OZ217343
Coordinates   223308..224324 (+) Length   338 a.a.
NCBI ID   WP_218774887.1    Uniprot ID   -
Organism   Streptococcus mitis isolate S. mitis G22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 218308..229324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLV7Z_RS01150 (SMIG22_02250) nagA 219454..220605 (+) 1152 WP_218774871.1 N-acetylglucosamine-6-phosphate deacetylase -
  ACLV7Z_RS01155 (SMIG22_02260) - 220768..221826 (+) 1059 WP_004257473.1 zinc-dependent alcohol dehydrogenase family protein -
  ACLV7Z_RS01160 (SMIG22_02270) - 221977..222342 (+) 366 WP_000286413.1 DUF1033 family protein -
  ACLV7Z_RS01165 (SMIG22_02280) comGA/cglA/cilD 222419..223360 (+) 942 WP_218774872.1 competence type IV pilus ATPase ComGA Machinery gene
  ACLV7Z_RS01170 (SMIG22_02290) comGB/cglB 223308..224324 (+) 1017 WP_218774887.1 competence type IV pilus assembly protein ComGB Machinery gene
  ACLV7Z_RS01175 (SMIG22_02300) comGC/cglC 224326..224649 (+) 324 WP_000738641.1 comG operon protein ComGC Machinery gene
  ACLV7Z_RS01180 (SMIG22_02310) comGD/cglD 224612..225046 (+) 435 WP_033628889.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACLV7Z_RS01185 (SMIG22_02320) comGE/cglE 225009..225311 (+) 303 WP_071478895.1 competence type IV pilus minor pilin ComGE Machinery gene
  ACLV7Z_RS01190 (SMIG22_02330) comGF/cglF 225274..225735 (+) 462 WP_218774873.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACLV7Z_RS01195 (SMIG22_02340) comGG/cglG 225713..226126 (+) 414 WP_218774874.1 competence type IV pilus minor pilin ComGG Machinery gene
  ACLV7Z_RS01200 (SMIG22_02350) - 226159..226746 (+) 588 WP_218774875.1 class I SAM-dependent methyltransferase -
  ACLV7Z_RS01205 (SMIG22_02360) comYH 226807..227760 (+) 954 WP_218774876.1 class I SAM-dependent methyltransferase Machinery gene
  ACLV7Z_RS01210 (SMIG22_02370) - 227810..229000 (+) 1191 WP_173306883.1 acetate kinase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38511.52 Da        Isoelectric Point: 9.7259

>NTDB_id=1170168 ACLV7Z_RS01170 WP_218774887.1 223308..224324(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis G22]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLNKQCVTQMRTGLSQGKSFSEIMDSLGFSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGFVSILALLALTFYKRSSKMRVFSILARLPFLGIFVQTYLTAYYAREWGNMISQGMELTQIFQIMQEQGSHLFKEI
GQDLAQSLQNGREFSQTIATYPFFKKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFIRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1170168 ACLV7Z_RS01170 WP_218774887.1 223308..224324(+) (comGB/cglB) [Streptococcus mitis isolate S. mitis G22]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTGTTTAA
CAATCTCTTTTCCAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCCTTGTTGAACAAGCAGTGTG
TGACCCAGATGCGCACGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATAATGGACAGCTTGGGATTTTCAAGTGCCATT
GTCACTCAGTTATCCCTAGCGGAAGTTCATGGGAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
TGCCAAGGTTAAGAAAAAACTAATTGAAGTAGCGACCTATCCCTTGATTTTGTTGGGATTTCTTCTCTTAATTATGCTGG
GACTACGGAACTATCTACTACCACAACTGGATAGTAGCAATATTGCTACCCAAATCATTGGCAATCTACCACAAATTTTT
CTAGGAATGGTAGGGTTTGTTTCCATACTTGCCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATTTTAGCACGCCTTCCCTTTCTTGGAATCTTTGTGCAGACTTATCTGACAGCTTATTATGCGCGTGAATGGGGGA
ATATGATTTCGCAGGGAATGGAGCTGACGCAGATTTTTCAAATCATGCAGGAACAAGGTTCCCATCTCTTTAAAGAAATC
GGTCAAGATTTAGCTCAATCCCTACAAAATGGCCGCGAATTTTCTCAAACCATAGCAACCTATCCTTTCTTTAAGAAAGA
GTTGAGTCTTATTATCGAGTATGGTGAAGTCAAGTCCAAGCTGGGGAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTATCCGAGTTAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus mitis SK321

96.45

100

0.965
  comGB/cglB Streptococcus mitis NCTC 12261

94.97

100

0.95
  comGB/cglB Streptococcus pneumoniae Rx1

94.97

100

0.95
  comGB/cglB Streptococcus pneumoniae D39

94.97

100

0.95
  comGB/cglB Streptococcus pneumoniae R6

94.97

100

0.95
  comGB/cglB Streptococcus pneumoniae TIGR4

94.97

100

0.95
  comYB Streptococcus gordonii str. Challis substr. CH1

73.214

99.408

0.728
  comYB Streptococcus mutans UA140

57.911

93.491

0.541
  comYB Streptococcus mutans UA159

57.911

93.491

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment