Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QM552_RS08460 Genome accession   NZ_OW011623
Coordinates   1866804..1867937 (-) Length   377 a.a.
NCBI ID   WP_004292190.1    Uniprot ID   N6X775
Organism   Thauera humireducens strain Piv1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1861804..1872937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QM552_RS08440 (C4PIVTH_1761) fdh3B 1863519..1864151 (+) 633 WP_282810010.1 formate dehydrogenase FDH3 subunit beta -
  QM552_RS08445 (C4PIVTH_1762) - 1864148..1864384 (+) 237 WP_004292193.1 hypothetical protein -
  QM552_RS08450 (C4PIVTH_1763) - 1864395..1865417 (+) 1023 WP_282810011.1 formate dehydrogenase subunit gamma -
  QM552_RS08455 (C4PIVTH_1764) - 1865581..1866750 (+) 1170 WP_282810012.1 class I SAM-dependent RNA methyltransferase -
  QM552_RS08460 (C4PIVTH_1765) pilU 1866804..1867937 (-) 1134 WP_004292190.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QM552_RS08465 (C4PIVTH_1766) pilT 1868019..1869062 (-) 1044 WP_038012416.1 type IV pilus twitching motility protein PilT Machinery gene
  QM552_RS08470 (C4PIVTH_1767) - 1869154..1869855 (+) 702 WP_038012414.1 YggS family pyridoxal phosphate-dependent enzyme -
  QM552_RS08475 (C4PIVTH_1768) proC 1869902..1870726 (+) 825 WP_004292188.1 pyrroline-5-carboxylate reductase -
  QM552_RS08480 (C4PIVTH_1769) - 1870733..1871314 (+) 582 WP_004292187.1 YggT family protein -
  QM552_RS08485 (C4PIVTH_1770) - 1871417..1872697 (-) 1281 WP_282810013.1 dihydroorotase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42151.46 Da        Isoelectric Point: 6.7013

>NTDB_id=1152195 QM552_RS08460 WP_004292190.1 1866804..1867937(-) (pilU) [Thauera humireducens strain Piv1]
MERDQALKFMHDLLRLMLQKNGSDLFITAGFPPAVKVDGRITPQSNQALQPQHTAELARAIMNDRQAAEFEASKECNFGI
SPAGIGRFRANAFVQQGKVGLVLRTIPQRIPKFDELGLPPVLTDIAMSKRGLVIFVGGTGTGKTTSLASMVDYRNENSYG
HIITVEDPIEFVHAHKNCIVSQREIGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMDYAIAFAETGHLCLATLHANSA
NQAIDRIINFFPEERRQQLLMDLSLNLRAMVSQRLLPLKDRKGRVPAVEVMLNSPLISDLIFKGNVPEIKEIMKRSRELG
MQTFDQALFDLYEAQKISYEDALRNADSINDLRLQIKLHSKLGERDLSSGTENLGIV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1152195 QM552_RS08460 WP_004292190.1 1866804..1867937(-) (pilU) [Thauera humireducens strain Piv1]
ATGGAACGCGACCAGGCACTCAAGTTCATGCACGACCTGCTGCGGCTGATGCTGCAGAAGAACGGCTCCGACCTCTTCAT
CACCGCGGGCTTTCCGCCGGCCGTGAAGGTGGACGGCCGCATCACGCCGCAGTCCAACCAGGCACTGCAACCGCAGCACA
CGGCCGAACTGGCCCGCGCGATCATGAACGACCGCCAGGCTGCCGAATTCGAAGCCAGCAAGGAATGCAACTTCGGCATC
TCGCCGGCCGGCATCGGCCGCTTCCGCGCCAATGCCTTCGTGCAGCAGGGCAAGGTCGGGCTGGTGTTGCGGACCATCCC
GCAGCGCATCCCGAAATTCGATGAGCTCGGCCTGCCGCCCGTGCTGACCGACATCGCGATGAGCAAGCGCGGGCTGGTGA
TCTTCGTCGGCGGCACCGGCACCGGCAAGACGACCTCGCTGGCGTCGATGGTCGACTACCGCAACGAGAACAGCTACGGC
CACATCATCACGGTCGAGGACCCGATCGAGTTCGTGCATGCGCACAAGAACTGCATCGTTTCGCAGCGCGAGATCGGCAT
CGACACCGACTCATGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTGATGGGCGAGATCCGCG
ACCGCGAGACGATGGACTACGCGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTCGCGACACTGCACGCCAACAGCGCC
AACCAGGCCATCGACCGCATCATCAACTTCTTCCCCGAGGAACGCCGCCAGCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCGCAGCGCCTGCTGCCACTGAAGGACCGCAAGGGCCGGGTGCCGGCCGTCGAGGTGATGCTCAATT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCAACGTGCCCGAGATCAAGGAGATCATGAAGCGCTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTCTACGAGGCGCAGAAGATCTCGTATGAGGACGCGCTGCGCAACGCCGA
CTCGATCAACGACCTGCGCCTGCAGATCAAGCTGCACAGCAAGCTGGGCGAGCGCGATCTCAGCAGCGGCACCGAGAACC
TCGGCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6X775

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.431

96.021

0.599
  pilU Acinetobacter baylyi ADP1

60.632

92.308

0.56
  pilU Vibrio cholerae strain A1552

55.493

94.164

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.075

88.859

0.401
  pilT Legionella pneumophila strain Lp02

43.807

87.798

0.385
  pilT Legionella pneumophila strain ERS1305867

43.807

87.798

0.385
  pilT Acinetobacter nosocomialis M2

42.598

87.798

0.374
  pilT Acinetobacter baumannii D1279779

42.598

87.798

0.374
  pilT Acinetobacter baumannii strain A118

42.598

87.798

0.374
  pilT Pseudomonas aeruginosa PAK

41.791

88.859

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.859

0.371
  pilT Acinetobacter baylyi ADP1

43.438

84.881

0.369

Multiple sequence alignment