Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CKV79_RS04125 Genome accession   NZ_LT906451
Coordinates   880866..882296 (+) Length   476 a.a.
NCBI ID   WP_051546119.1    Uniprot ID   A0A0W0VXF5
Organism   Legionella lansingensis strain NCTC12830     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 875866..887296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV79_RS04100 (SAMEA44548918_00829) - 876960..877937 (-) 978 WP_028372872.1 hypothetical protein -
  CKV79_RS04105 (SAMEA44548918_00830) pilM 878195..879232 (+) 1038 WP_028372871.1 type IV pilus biogenesis protein PilM -
  CKV79_RS04110 (SAMEA44548918_00831) - 879235..879792 (+) 558 WP_028372870.1 PilN domain-containing protein -
  CKV79_RS04115 (SAMEA44548918_00832) - 879782..880372 (+) 591 WP_028372869.1 type 4a pilus biogenesis protein PilO -
  CKV79_RS04120 (SAMEA44548918_00833) - 880483..880869 (+) 387 WP_231950209.1 pilus assembly protein PilP -
  CKV79_RS04125 (SAMEA44548918_00834) pilQ 880866..882296 (+) 1431 WP_051546119.1 type IV pilus secretin PilQ Machinery gene
  CKV79_RS04130 (SAMEA44548918_00835) aroK 882438..882965 (+) 528 WP_028372868.1 shikimate kinase AroK -
  CKV79_RS04135 (SAMEA44548918_00836) aroB 882952..884061 (+) 1110 WP_028372867.1 3-dehydroquinate synthase -
  CKV79_RS04140 (SAMEA44548918_00837) - 884065..885594 (+) 1530 WP_028372866.1 AAA family ATPase -
  CKV79_RS04145 pnuC 885761..886342 (-) 582 WP_051546118.1 nicotinamide riboside transporter PnuC -
  CKV79_RS04150 (SAMEA44548918_00839) - 886568..886996 (+) 429 WP_028372865.1 universal stress protein -

Sequence

Protein


Download         Length: 476 a.a.        Molecular weight: 53020.64 Da        Isoelectric Point: 10.1411

>NTDB_id=1147397 CKV79_RS04125 WP_051546119.1 880866..882296(+) (pilQ) [Legionella lansingensis strain NCTC12830]
MILRNIFLVLFIFFLFYSKLLAQPSLVIPIVAGKSEKDKMTRKDVYHKRISLNFQDIEVRSALQLLAEFAGINIVVSDTV
AGNITISLNDIPWDQALNIILTTRSLEKKRMGNIIFIAPATEITERRKKIEEAQLKLNDMTPLQSELFQINYAKASEIAN
ILQAKNETLLSTRGKMAIDARTNTLWIQESTRRLAVIRKLIEYFDVPVKQVLIEARIVNVTKDFAQDLGLHFGLSRGRLS
DARKDNITQLTEKTASPSATPLADRLNFDIGNIPIGTSTASLGIALAKLGDGILLDLELSALESEGRGEVISSPRLIATN
QQPAVIESGEEIPYQEATSSGATAVSFKKAVLSLKVTPQITPDGKILMDLKINQDIPTPKVFNGVPTILTKEIQTNVLAC
NGQTIVLGGIYKQDKNNEINRVPFLGELPMVGGLFRNQSTSIRNEELLIFITPRIISNNLRQKPLRRQRQKTKERL

Nucleotide


Download         Length: 1431 bp        

>NTDB_id=1147397 CKV79_RS04125 WP_051546119.1 880866..882296(+) (pilQ) [Legionella lansingensis strain NCTC12830]
TTGATTTTGCGAAACATATTTTTAGTATTGTTCATTTTTTTTCTTTTTTATTCTAAATTACTTGCACAACCCAGTTTAGT
CATACCGATAGTTGCAGGAAAGAGCGAAAAAGATAAAATGACAAGAAAGGATGTTTATCACAAACGAATTTCACTAAATT
TTCAAGATATTGAGGTGAGATCCGCTTTGCAATTATTGGCTGAATTTGCTGGCATCAACATAGTTGTGAGTGATACAGTA
GCGGGCAATATAACAATTAGCTTGAATGATATACCCTGGGACCAGGCTTTAAATATTATTCTAACAACCCGATCCTTAGA
GAAAAAACGAATGGGCAATATCATATTTATCGCGCCAGCCACTGAAATAACAGAGCGTAGGAAAAAAATTGAAGAAGCTC
AACTTAAGTTAAACGATATGACCCCGTTGCAATCTGAGCTCTTTCAAATCAATTATGCGAAGGCTTCTGAAATTGCCAAT
ATATTGCAAGCAAAGAATGAAACCCTTCTGTCTACTCGGGGTAAGATGGCTATTGATGCAAGAACCAATACACTTTGGAT
ACAAGAGAGTACCAGGCGACTTGCCGTTATAAGAAAATTGATTGAGTATTTTGATGTTCCAGTGAAACAAGTATTGATAG
AAGCACGTATTGTTAATGTAACAAAAGATTTTGCTCAGGATTTAGGGCTGCATTTTGGTCTCTCCAGAGGCAGACTTAGT
GATGCGCGCAAGGATAATATCACGCAATTGACAGAAAAGACTGCTTCGCCATCAGCAACTCCATTAGCAGATCGCTTAAA
TTTTGATATTGGAAACATACCTATAGGAACTTCAACAGCCTCTCTTGGGATAGCTCTTGCCAAGTTGGGTGATGGGATTT
TGTTGGATTTGGAGCTTTCCGCCCTAGAAAGTGAGGGTCGAGGAGAAGTCATTTCCAGTCCACGATTGATAGCGACTAAC
CAGCAGCCAGCAGTGATTGAATCAGGAGAAGAAATTCCATACCAAGAAGCAACTTCGAGTGGCGCCACAGCTGTATCTTT
TAAAAAAGCTGTATTGAGCCTGAAAGTGACCCCACAGATCACCCCTGATGGTAAAATTTTAATGGATTTAAAAATTAACC
AGGATATACCCACTCCCAAGGTATTTAATGGTGTCCCAACGATTCTAACGAAGGAAATTCAAACAAATGTGTTAGCCTGT
AATGGACAAACCATTGTTCTCGGTGGAATTTATAAACAAGATAAAAATAACGAGATCAATCGTGTCCCTTTTCTAGGAGA
ATTACCAATGGTAGGCGGATTATTTAGAAATCAGTCGACATCAATTAGAAATGAGGAGTTGCTCATTTTCATTACTCCTA
GGATAATATCAAACAATTTACGGCAAAAACCGTTAAGAAGGCAAAGGCAAAAAACTAAGGAACGTCTCTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(146-210)

Bacterial type II and III secretion system protein (Secretin)

(301-456)

Secretin and TonB N terminus short domain (STN)

(72-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0VXF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain ERS1305867

61.959

92.227

0.571
  pilQ Legionella pneumophila strain Lp02

61.959

92.227

0.571
  comQ Acinetobacter baylyi ADP1

44.395

93.697

0.416
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

47.596

87.395

0.416
  pilQ Vibrio cholerae strain A1552

47.596

87.395

0.416
  pilQ Vibrio campbellii strain DS40M4

44.731

89.706

0.401
  pilQ Acinetobacter baumannii D1279779

41.164

97.479

0.401
  pilQ Acinetobacter baumannii strain A118

41.164

97.479

0.401
  pilQ Pseudomonas aeruginosa PAK

40.359

93.697

0.378
  pilQ Neisseria gonorrhoeae strain FA1090

40.09

93.277

0.374
  pilQ Neisseria meningitidis MC58

39.45

91.597

0.361
  pilQ Neisseria meningitidis M1080

39.45

91.597

0.361

Multiple sequence alignment