Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DQL20_RS01280 Genome accession   NZ_LS483444
Coordinates   214289..215230 (+) Length   313 a.a.
NCBI ID   WP_006597118.1    Uniprot ID   -
Organism   Streptococcus australis strain NCTC13166     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 209289..220230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL20_RS01255 (NCTC11371_00249) - 210246..211034 (+) 789 WP_006595143.1 hypothetical protein -
  DQL20_RS01260 (NCTC11371_00250) - 211044..211184 (+) 141 WP_006595142.1 hypothetical protein -
  DQL20_RS01265 (NCTC11371_00251) - 211240..212487 (+) 1248 WP_006597122.1 glycosyltransferase family 2 protein -
  DQL20_RS01270 (NCTC11371_00252) - 212496..213599 (+) 1104 WP_006595140.1 glycosyl hydrolase family 8 -
  DQL20_RS01275 (NCTC11371_00253) - 213806..214183 (+) 378 WP_006595139.1 DUF1033 family protein -
  DQL20_RS01280 (NCTC11371_00254) comYA 214289..215230 (+) 942 WP_006597118.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL20_RS01285 (NCTC11371_00255) comYB 215163..216194 (+) 1032 WP_408022506.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL20_RS01290 (NCTC11371_00256) comGC/cglC 216191..216508 (+) 318 WP_006595136.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL20_RS01295 comGD 216477..216902 (+) 426 WP_040802795.1 competence type IV pilus minor pilin ComGD -
  DQL20_RS01300 (NCTC11371_00258) comGE 216868..217161 (+) 294 WP_006595134.1 competence type IV pilus minor pilin ComGE -
  DQL20_RS01305 (NCTC11371_00259) comGF/cglF 217145..217597 (+) 453 WP_040803180.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL20_RS01310 (NCTC11371_00260) comGG 217563..218009 (+) 447 WP_006595132.1 competence type IV pilus minor pilin ComGG -
  DQL20_RS01315 (NCTC11371_00261) comYH 218041..218994 (+) 954 WP_006597125.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35662.73 Da        Isoelectric Point: 5.2747

>NTDB_id=1141712 DQL20_RS01280 WP_006597118.1 214289..215230(+) (comYA) [Streptococcus australis strain NCTC13166]
MVQEIAQSIIRMASNAEAQDIYFVPRASDYQLFLRVGDERRFVETYSQEQMVAVISHFKFMAGMNVGERRRSQLGSCDYS
LGDKVLSLRLSTVGDYRGYESLVIRLLHNEDRELRFWFDQLQELEEKVSKRGLYLFAGPVGSGKTTLMHALAKKKFSGQQ
VMSIEDPVEIKQEEMLQLQLNEAIGMTYDSLIKLSLRHRPDLLLIGEIRDTETARAVIRASLTGVTVFSTIHAKSIPGVY
ERLLELGVSEEELRIVLQGICYQRLIKGGGVTDFAIQDYQNHSSQQWNQQMDALYEAGHIELDQAQAEKIIDC

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1141712 DQL20_RS01280 WP_006597118.1 214289..215230(+) (comYA) [Streptococcus australis strain NCTC13166]
ATGGTTCAAGAAATTGCACAATCCATAATTCGAATGGCATCGAATGCCGAAGCTCAGGATATTTATTTTGTTCCTCGAGC
ATCAGACTATCAGCTCTTTCTAAGGGTTGGGGATGAGCGACGCTTTGTCGAGACCTATTCGCAAGAACAAATGGTAGCGG
TCATCAGTCATTTTAAGTTTATGGCAGGGATGAATGTGGGGGAAAGGCGCCGGAGTCAATTGGGCTCCTGTGACTATTCT
CTAGGGGATAAGGTCTTGTCCTTACGCTTGTCTACTGTGGGAGACTATAGGGGATACGAGAGTTTAGTTATTCGCCTGCT
CCACAATGAGGACCGGGAGCTCCGTTTCTGGTTCGACCAACTTCAAGAGCTAGAAGAGAAGGTGAGTAAGCGGGGGCTCT
ACTTATTTGCGGGTCCAGTCGGATCGGGGAAGACAACACTGATGCATGCCCTGGCCAAGAAAAAATTTTCGGGGCAACAA
GTCATGTCGATTGAAGATCCCGTAGAAATTAAGCAAGAAGAGATGCTGCAACTGCAGCTCAATGAAGCGATTGGCATGAC
TTATGATAGCTTGATTAAACTGTCTCTGCGTCATCGCCCGGATCTCCTCCTCATCGGAGAAATTCGTGACACGGAGACAG
CTCGTGCAGTTATTCGGGCTAGTCTGACAGGTGTGACGGTCTTTTCTACCATCCATGCCAAGAGCATTCCTGGGGTTTAT
GAACGCCTACTGGAGTTGGGGGTGAGTGAGGAGGAGTTACGGATTGTCCTTCAAGGGATCTGTTACCAGCGTTTGATAAA
GGGAGGAGGTGTCACTGACTTTGCCATCCAAGATTACCAAAACCACTCCAGTCAACAGTGGAACCAGCAAATGGATGCGC
TTTATGAAGCGGGACATATTGAGCTGGATCAGGCGCAAGCCGAAAAAATTATCGATTGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus gordonii str. Challis substr. CH1

75.806

99.042

0.751
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

72.903

99.042

0.722
  comGA/cglA/cilD Streptococcus pneumoniae D39

71.935

99.042

0.712
  comGA/cglA/cilD Streptococcus pneumoniae R6

71.935

99.042

0.712
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

71.935

99.042

0.712
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

71.935

99.042

0.712
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.179

99.681

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

55.305

99.361

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.877

88.498

0.371

Multiple sequence alignment