Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   DQL20_RS01285 Genome accession   NZ_LS483444
Coordinates   215163..216194 (+) Length   343 a.a.
NCBI ID   WP_408022506.1    Uniprot ID   -
Organism   Streptococcus australis strain NCTC13166     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 210163..221194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL20_RS01255 (NCTC11371_00249) - 210246..211034 (+) 789 WP_006595143.1 hypothetical protein -
  DQL20_RS01260 (NCTC11371_00250) - 211044..211184 (+) 141 WP_006595142.1 hypothetical protein -
  DQL20_RS01265 (NCTC11371_00251) - 211240..212487 (+) 1248 WP_006597122.1 glycosyltransferase family 2 protein -
  DQL20_RS01270 (NCTC11371_00252) - 212496..213599 (+) 1104 WP_006595140.1 glycosyl hydrolase family 8 -
  DQL20_RS01275 (NCTC11371_00253) - 213806..214183 (+) 378 WP_006595139.1 DUF1033 family protein -
  DQL20_RS01280 (NCTC11371_00254) comYA 214289..215230 (+) 942 WP_006597118.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL20_RS01285 (NCTC11371_00255) comYB 215163..216194 (+) 1032 WP_408022506.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL20_RS01290 (NCTC11371_00256) comGC/cglC 216191..216508 (+) 318 WP_006595136.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL20_RS01295 comGD 216477..216902 (+) 426 WP_040802795.1 competence type IV pilus minor pilin ComGD -
  DQL20_RS01300 (NCTC11371_00258) comGE 216868..217161 (+) 294 WP_006595134.1 competence type IV pilus minor pilin ComGE -
  DQL20_RS01305 (NCTC11371_00259) comGF/cglF 217145..217597 (+) 453 WP_040803180.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL20_RS01310 (NCTC11371_00260) comGG 217563..218009 (+) 447 WP_006595132.1 competence type IV pilus minor pilin ComGG -
  DQL20_RS01315 (NCTC11371_00261) comYH 218041..218994 (+) 954 WP_006597125.1 class I SAM-dependent methyltransferase Machinery gene
  DQL20_RS01320 (NCTC11371_00262) - 219043..220239 (+) 1197 WP_006595130.1 acetate kinase -
  DQL20_RS01325 (NCTC11371_00263) - 220385..221074 (+) 690 WP_006595129.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 39317.56 Da        Isoelectric Point: 9.8568

>NTDB_id=1141713 DQL20_RS01285 WP_408022506.1 215163..216194(+) (comYB) [Streptococcus australis strain NCTC13166]
MRFMKRDILSWIRRKPKKLSIAKQEHVIELFHNLYSSGFHLSEVIDFLRRSQLVEADFVERMQVGLANGKPLSAILSDLG
FSDQVTTQLALAEQHGNVTLSLEKIRSYLENLTQVRKKLVEVATYPVLLLGFLILIMLGLKNYLLPQLENQNGATRLISI
FPQYVLLGSVLLTIGVLMLLFLGRRAKRLALVSCLARFPFLRPFLQDYLTAFYAREWGSMIAQGLELNQIFPVMQGQRSR
LFREIGQDLEVALARGISFSDHIQTYPFFKRELALMIEYGEAKSKLGSELEIYADKTWAGFFHRLNKAMNVVQPLVFIFV
ALMIVLLYAAMLLPIYENLEVPL

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=1141713 DQL20_RS01285 WP_408022506.1 215163..216194(+) (comYB) [Streptococcus australis strain NCTC13166]
ATGCGCTTTATGAAGCGGGACATATTGAGCTGGATCAGGCGCAAGCCGAAAAAATTATCGATTGCTAAGCAAGAACATGT
CATTGAACTCTTTCACAATCTCTACAGCAGTGGCTTTCATCTATCAGAGGTCATCGACTTTCTTCGGCGCAGTCAATTGG
TAGAGGCAGATTTTGTCGAGAGGATGCAGGTCGGACTTGCCAACGGGAAGCCCCTGTCAGCCATTCTCTCTGATCTAGGT
TTCTCCGATCAGGTAACGACTCAATTGGCACTGGCGGAGCAGCATGGAAATGTTACCTTAAGTTTAGAAAAGATCCGTAG
CTACTTGGAAAATCTGACCCAGGTTCGAAAGAAACTAGTTGAGGTGGCGACCTATCCCGTGCTCTTGTTGGGATTTTTGA
TCTTGATTATGCTGGGGCTGAAAAATTATCTCTTGCCCCAGTTGGAGAATCAAAATGGGGCGACACGACTGATTAGTATC
TTTCCCCAGTATGTTTTGTTAGGGAGTGTGTTGCTGACGATTGGAGTTTTGATGCTCCTTTTCCTAGGCCGTCGTGCCAA
ACGGCTGGCCCTGGTTTCATGTTTGGCTAGATTTCCTTTTCTTCGCCCCTTCTTACAGGATTATCTGACCGCTTTTTATG
CTCGAGAATGGGGAAGTATGATTGCCCAAGGGTTGGAGCTGAATCAAATTTTTCCTGTGATGCAGGGGCAACGTTCCCGT
CTCTTTCGAGAGATTGGACAGGATTTAGAAGTAGCCTTGGCTAGGGGGATTTCTTTTTCTGACCATATTCAGACTTATCC
TTTCTTTAAACGAGAGCTGGCCCTCATGATTGAATATGGGGAAGCCAAATCCAAACTCGGTAGTGAGTTGGAGATCTATG
CAGACAAGACTTGGGCAGGCTTTTTCCATCGTTTGAACAAGGCCATGAATGTCGTCCAACCGCTGGTCTTTATCTTTGTC
GCTCTCATGATTGTTTTACTCTATGCAGCCATGTTGCTGCCGATTTATGAAAATTTGGAGGTTCCTTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(214-334)

Type II secretion system (T2SS), protein F (T2SSF)

(29-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus gordonii str. Challis substr. CH1

60.87

100

0.612
  comGB/cglB Streptococcus mitis NCTC 12261

60.714

97.959

0.595
  comGB/cglB Streptococcus mitis SK321

59.821

97.959

0.586
  comGB/cglB Streptococcus pneumoniae Rx1

59.226

97.959

0.58
  comGB/cglB Streptococcus pneumoniae D39

59.226

97.959

0.58
  comGB/cglB Streptococcus pneumoniae R6

59.226

97.959

0.58
  comGB/cglB Streptococcus pneumoniae TIGR4

59.226

97.959

0.58
  comYB Streptococcus mutans UA159

57.434

100

0.574
  comYB Streptococcus mutans UA140

57.434

100

0.574
  comGB Lactococcus lactis subsp. cremoris KW2

49.271

100

0.493

Multiple sequence alignment