Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   DQN32_RS06595 Genome accession   NZ_LS483432
Coordinates   1262962..1263381 (-) Length   139 a.a.
NCBI ID   WP_011054759.1    Uniprot ID   A0A5S4TJJ6
Organism   Streptococcus pyogenes strain NCTC13745     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1230242..1271678 1262962..1263381 within 0


Gene organization within MGE regions


Location: 1230242..1271678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN32_RS06355 (NCTC13745_01266) prx 1230242..1230424 (-) 183 WP_011054726.1 hypothetical protein Regulator
  DQN32_RS06365 (NCTC13745_01268) - 1230770..1231345 (-) 576 WP_011054727.1 hypothetical protein -
  DQN32_RS06370 (NCTC13745_01269) spek 1231821..1232600 (-) 780 WP_011054728.1 streptococcal pyrogenic exotoxin SpeK -
  DQN32_RS06375 (NCTC13745_01270) - 1232904..1233770 (-) 867 WP_076639317.1 DUF334 domain-containing protein -
  DQN32_RS06380 (NCTC13745_01271) - 1233758..1234282 (-) 525 WP_011017840.1 Panacea domain-containing protein -
  DQN32_RS06385 (NCTC13745_01272) - 1234422..1235624 (-) 1203 WP_011054730.1 glucosaminidase domain-containing protein -
  DQN32_RS06390 (NCTC13745_01274) - 1235740..1235967 (-) 228 WP_011054444.1 phage holin -
  DQN32_RS06395 (NCTC13745_01275) - 1235964..1236236 (-) 273 WP_011017397.1 hypothetical protein -
  DQN32_RS06400 (NCTC13745_01276) - 1236248..1236880 (-) 633 WP_011054443.1 hypothetical protein -
  DQN32_RS06405 (NCTC13745_01277) - 1236883..1237311 (-) 429 WP_002988448.1 DUF1617 family protein -
  DQN32_RS06410 (NCTC13745_01278) - 1237323..1239212 (-) 1890 WP_111681974.1 gp58-like family protein -
  DQN32_RS06415 (NCTC13745_01279) - 1239223..1239537 (-) 315 WP_063812987.1 hypothetical protein -
  DQN32_RS06420 (NCTC13745_01280) - 1239539..1240753 (-) 1215 WP_111681975.1 hypothetical protein -
  DQN32_RS06425 (NCTC13745_01281) - 1240750..1242897 (-) 2148 WP_076639318.1 phage tail spike protein -
  DQN32_RS06430 (NCTC13745_01282) - 1242894..1243610 (-) 717 WP_076639319.1 distal tail protein Dit -
  DQN32_RS06435 (NCTC13745_01283) - 1243607..1246867 (-) 3261 WP_076639320.1 tape measure protein -
  DQN32_RS06440 (NCTC13745_01284) - 1246857..1247438 (-) 582 WP_011054739.1 bacteriophage Gp15 family protein -
  DQN32_RS06445 (NCTC13745_01285) - 1247442..1247876 (-) 435 WP_011054740.1 hypothetical protein -
  DQN32_RS06450 (NCTC13745_01286) - 1247915..1248400 (-) 486 WP_011054741.1 phage tail tube protein -
  DQN32_RS06455 (NCTC13745_01287) - 1248400..1248798 (-) 399 WP_010922084.1 minor capsid protein -
  DQN32_RS06460 (NCTC13745_01288) - 1248795..1249151 (-) 357 WP_010922083.1 minor capsid protein -
  DQN32_RS06465 (NCTC13745_01289) - 1249151..1249483 (-) 333 WP_010922082.1 minor capsid protein -
  DQN32_RS06470 (NCTC13745_01290) - 1249473..1249889 (-) 417 WP_011054743.1 hypothetical protein -
  DQN32_RS06475 (NCTC13745_01291) - 1249943..1250761 (-) 819 WP_010922080.1 N4-gp56 family major capsid protein -
  DQN32_RS06480 (NCTC13745_01292) - 1250765..1251379 (-) 615 WP_011106689.1 hypothetical protein -
  DQN32_RS06485 (NCTC13745_01293) - 1251505..1251771 (-) 267 WP_011054745.1 hypothetical protein -
  DQN32_RS06490 (NCTC13745_01294) - 1251833..1252072 (-) 240 WP_002986829.1 hypothetical protein -
  DQN32_RS06495 (NCTC13745_01295) - 1252044..1253522 (-) 1479 WP_011054746.1 phage minor capsid protein -
  DQN32_RS06500 (NCTC13745_01296) - 1253527..1255029 (-) 1503 WP_002986832.1 phage portal protein -
  DQN32_RS06505 (NCTC13745_01297) - 1255043..1256334 (-) 1292 Protein_1212 PBSX family phage terminase large subunit -
  DQN32_RS06510 (NCTC13745_01298) - 1256337..1256810 (-) 474 WP_011054747.1 hypothetical protein -
  DQN32_RS06515 (NCTC13745_01299) - 1256861..1257238 (-) 378 WP_002986841.1 ASCH domain-containing protein -
  DQN32_RS06520 - 1257299..1257730 (-) 432 WP_074375320.1 GNAT family N-acetyltransferase -
  DQN32_RS06525 (NCTC13745_01300) - 1257708..1258226 (-) 519 WP_076639322.1 ParB N-terminal domain-containing protein -
  DQN32_RS06530 - 1258307..1258564 (+) 258 WP_011054748.1 hypothetical protein -
  DQN32_RS06550 (NCTC13745_01301) - 1259203..1259643 (-) 441 WP_011017866.1 ArpU family phage packaging/lysis transcriptional regulator -
  DQN32_RS09475 (NCTC13745_01302) - 1259917..1260087 (-) 171 WP_164997036.1 hypothetical protein -
  DQN32_RS06555 (NCTC13745_01303) - 1260084..1260590 (-) 507 WP_011054751.1 DUF1642 domain-containing protein -
  DQN32_RS09480 (NCTC13745_01304) - 1260587..1260757 (-) 171 WP_011054752.1 hypothetical protein -
  DQN32_RS06560 (NCTC13745_01305) - 1260754..1261158 (-) 405 WP_011054753.1 YopX family protein -
  DQN32_RS06565 (NCTC13745_01306) - 1261168..1261437 (-) 270 WP_011054754.1 hypothetical protein -
  DQN32_RS06570 (NCTC13745_01307) - 1261434..1261718 (-) 285 WP_011054755.1 DUF3310 domain-containing protein -
  DQN32_RS06575 (NCTC13745_01308) - 1261712..1261963 (-) 252 WP_011054756.1 hypothetical protein -
  DQN32_RS06580 (NCTC13745_01309) - 1261960..1262316 (-) 357 WP_011054757.1 hypothetical protein -
  DQN32_RS06585 (NCTC13745_01310) - 1262313..1262753 (-) 441 WP_011054758.1 RusA family crossover junction endodeoxyribonuclease -
  DQN32_RS06590 - 1262753..1262956 (-) 204 WP_011106686.1 hypothetical protein -
  DQN32_RS06595 (NCTC13745_01311) ssbA 1262962..1263381 (-) 420 WP_011054759.1 single-stranded DNA-binding protein Machinery gene
  DQN32_RS06600 (NCTC13745_01312) - 1263374..1264048 (-) 675 WP_011054760.1 ERF family protein -
  DQN32_RS06605 (NCTC13745_01313) - 1264049..1264531 (-) 483 WP_011018142.1 siphovirus Gp157 family protein -
  DQN32_RS06610 (NCTC13745_01314) - 1264553..1264807 (-) 255 WP_011054761.1 hypothetical protein -
  DQN32_RS06615 (NCTC13745_01315) - 1264818..1264958 (-) 141 WP_011284979.1 hypothetical protein -
  DQN32_RS06620 (NCTC13745_01316) - 1264955..1265188 (-) 234 WP_011054762.1 hypothetical protein -
  DQN32_RS06625 (NCTC13745_01317) - 1265169..1265582 (-) 414 WP_011054763.1 DnaD domain protein -
  DQN32_RS06630 (NCTC13745_01318) - 1265704..1265961 (-) 258 WP_011106684.1 hypothetical protein -
  DQN32_RS06635 (NCTC13745_01319) - 1266055..1266240 (-) 186 WP_011054765.1 hypothetical protein -
  DQN32_RS06640 (NCTC13745_01320) - 1266269..1266526 (-) 258 WP_002988339.1 hypothetical protein -
  DQN32_RS06650 (NCTC13745_01322) - 1266772..1266939 (-) 168 WP_002986885.1 hypothetical protein -
  DQN32_RS06655 (NCTC13745_01323) - 1267015..1267215 (+) 201 WP_002986887.1 KTSC domain-containing protein -
  DQN32_RS09485 (NCTC13745_01324) - 1267212..1267361 (-) 150 WP_002986888.1 hypothetical protein -
  DQN32_RS06660 (NCTC13745_01325) - 1267394..1268122 (-) 729 WP_011054767.1 phage antirepressor KilAC domain-containing protein -
  DQN32_RS06665 (NCTC13745_01326) - 1268133..1268324 (-) 192 WP_002986891.1 hypothetical protein -
  DQN32_RS06670 (NCTC13745_01327) - 1269126..1269479 (+) 354 WP_002986893.1 helix-turn-helix domain-containing protein -
  DQN32_RS06675 (NCTC13745_01328) - 1269493..1269873 (+) 381 WP_002986894.1 ImmA/IrrE family metallo-endopeptidase -
  DQN32_RS06680 (NCTC13745_01329) - 1269884..1270405 (+) 522 WP_002986895.1 hypothetical protein -
  DQN32_RS06685 (NCTC13745_01330) - 1270581..1271678 (+) 1098 WP_015967409.1 site-specific integrase -

Sequence


Protein


Download         Length: 139 a.a.        Molecular weight: 15649.29 Da        Isoelectric Point: 4.8660

>NTDB_id=1141342 DQN32_RS06595 WP_011054759.1 1262962..1263381(-) (ssbA) [Streptococcus pyogenes strain NCTC13745]
MINNVVLVGRMTKDAELRYTASQVAVATFTLAVNRRFKEQNGEREADFINCVIWRQSAENLANWAKKGALIGVTGRIQTR
NYENQQGQRVYVTEVVADNFQMLESRNQQSGQGNSSQNDNSQPFGNSNPMDISDDDLPF

Nucleotide


Download         Length: 420 bp        

>NTDB_id=1141342 DQN32_RS06595 WP_011054759.1 1262962..1263381(-) (ssbA) [Streptococcus pyogenes strain NCTC13745]
ATGATTAATAATGTAGTACTAGTTGGTCGCATGACCAAGGACGCAGAGCTTCGCTATACAGCGAGTCAAGTAGCTGTAGC
TACGTTCACACTTGCGGTAAACCGCAGATTTAAAGAGCAAAACGGGGAGAGAGAAGCAGATTTCATTAACTGTGTTATCT
GGCGACAGTCTGCTGAAAATTTAGCCAACTGGGCTAAAAAAGGTGCTTTGATCGGAGTTACGGGTCGTATTCAGACACGT
AACTACGAAAACCAACAAGGACAACGTGTCTATGTAACAGAAGTTGTTGCAGATAATTTCCAAATGTTGGAAAGTCGTAA
TCAACAATCTGGTCAAGGTAACTCTTCGCAAAACGATAACAGTCAACCGTTTGGCAATTCAAACCCAATGGATATTTCAG
ACGATGATCTGCCGTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A5S4TJJ6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

54.857

100

0.691

  ssb Latilactobacillus sakei subsp. sakei 23K

55.882

100

0.683

  ssbB/cilA Streptococcus pneumoniae TIGR4

46.043

100

0.46

  ssbB/cilA Streptococcus pneumoniae Rx1

45.324

100

0.453

  ssbB/cilA Streptococcus mitis NCTC 12261

45.324

100

0.453

  ssbB/cilA Streptococcus pneumoniae D39

45.324

100

0.453

  ssbB/cilA Streptococcus pneumoniae R6

45.324

100

0.453

  ssbB/cilA Streptococcus mitis SK321

45.324

100

0.453

  ssbB Streptococcus sobrinus strain NIDR 6715-7

44.604

100

0.446

  ssbB Bacillus subtilis subsp. subtilis str. 168

54.955

79.856

0.439

  ssbA Streptococcus mutans UA159

41.727

100

0.417

  ssb Vibrio cholerae strain A1552

31.214

100

0.388

  ssb Glaesserella parasuis strain SC1401

28.814

100

0.367


Multiple sequence alignment