Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H5027_RS03145 Genome accession   NZ_LR861807
Coordinates   713347..714480 (-) Length   377 a.a.
NCBI ID   WP_016900968.1    Uniprot ID   A0A2N7V1C6
Organism   Xanthomonas arboricola pv. juglandis isolate 3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 708347..719480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H5027_RS03130 (XSP_000626) - 709599..710987 (-) 1389 WP_016900971.1 DEAD/DEAH box helicase -
  H5027_RS03135 (XSP_000627) - 711296..712354 (+) 1059 WP_180708324.1 fumarylacetoacetate hydrolase family protein -
  H5027_RS03140 (XSP_000628) maiA 712398..713063 (+) 666 WP_053045859.1 maleylacetoacetate isomerase -
  H5027_RS03145 (XSP_000629) pilU 713347..714480 (-) 1134 WP_016900968.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H5027_RS03150 (XSP_000630) - 714579..714962 (+) 384 WP_039525328.1 DUF4398 domain-containing protein -
  H5027_RS03155 (XSP_000631) - 714959..715849 (+) 891 WP_053045857.1 hypothetical protein -
  H5027_RS03160 (XSP_000632) - 716195..716413 (+) 219 WP_002814034.1 YdcH family protein -
  H5027_RS03165 (XSP_000633) - 716701..718071 (+) 1371 WP_053045855.1 pyridoxal-phosphate dependent enzyme -
  H5027_RS03170 (XSP_000634) - 718161..719357 (+) 1197 WP_053045854.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41754.11 Da        Isoelectric Point: 6.1401

>NTDB_id=1132468 H5027_RS03145 WP_016900968.1 713347..714480(-) (pilU) [Xanthomonas arboricola pv. juglandis isolate 3]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1132468 H5027_RS03145 WP_016900968.1 713347..714480(-) (pilU) [Xanthomonas arboricola pv. juglandis isolate 3]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCACCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCTTATTCAT
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATTGCCCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAAGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCATGCCTTCCAGAG
CGCGCTGAAAAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCGAGACCATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCGCTGCG
CCTGGTCAAGGGCGTGGACGGACGCCGCCGGCCGGCGACCGAAGTGCTGCTCAATACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGTCCGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTTCGCCTGTCCGAGGGCGGCTCCGGCGAGCACGACCCGTATGCGGATTACGAAACGGCAGCAGCGCCGAAGATCAGCC
ACGGCTTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7V1C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

48.889

95.491

0.467
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.979

100

0.446
  pilT Pseudomonas aeruginosa PAK

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

40.828

89.655

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366
  pilT Acinetobacter nosocomialis M2

40.828

89.655

0.366
  pilT Acinetobacter baumannii D1279779

40.828

89.655

0.366
  pilT Acinetobacter baylyi ADP1

40.294

90.186

0.363

Multiple sequence alignment