Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7A87_RS16655 Genome accession   NZ_LR861805
Coordinates   3954513..3955646 (+) Length   377 a.a.
NCBI ID   WP_119128315.1    Uniprot ID   -
Organism   Xanthomonas sp. CPBF 426 isolate     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3933728..3956648 3954513..3955646 within 0


Gene organization within MGE regions


Location: 3933728..3956648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7A87_RS16570 (XSP_003329) - 3933728..3934678 (+) 951 WP_102580563.1 glycosyltransferase family 2 protein -
  H7A87_RS16575 (XSP_003330) - 3934771..3935091 (+) 321 WP_232087983.1 GtrA family protein -
  H7A87_RS16580 (XSP_003331) - 3935132..3937312 (+) 2181 WP_147421081.1 hypothetical protein -
  H7A87_RS16585 (XSP_003332) - 3938211..3939152 (-) 942 WP_119128320.1 NAD-dependent epimerase/dehydratase family protein -
  H7A87_RS16590 (XSP_003333) gmd 3939136..3940170 (-) 1035 WP_012439287.1 GDP-mannose 4,6-dehydratase -
  H7A87_RS16595 (XSP_003334) - 3940248..3940847 (-) 600 WP_180496777.1 glycosyltransferase -
  H7A87_RS16600 (XSP_003335) - 3940883..3942631 (-) 1749 WP_119128319.1 glycosyltransferase -
  H7A87_RS16605 (XSP_003336) - 3942652..3943713 (-) 1062 WP_102580568.1 glycosyltransferase family 4 protein -
  H7A87_RS16610 (XSP_003337) - 3943763..3946051 (-) 2289 WP_232087869.1 methyltransferase domain-containing protein -
  H7A87_RS16615 (XSP_003338) - 3946091..3947377 (-) 1287 WP_102580569.1 ABC transporter ATP-binding protein -
  H7A87_RS16620 (XSP_003339) - 3947374..3948159 (-) 786 WP_180496778.1 ABC transporter permease -
  H7A87_RS16625 (XSP_003340) - 3948252..3949631 (-) 1380 WP_119128317.1 glycosyltransferase family 4 protein -
  H7A87_RS16630 (XSP_003341) - 3949628..3950827 (-) 1200 WP_119128316.1 cystathionine gamma-synthase -
  H7A87_RS16635 (XSP_003342) - 3950929..3952299 (-) 1371 WP_104583283.1 pyridoxal-phosphate dependent enzyme -
  H7A87_RS16640 (XSP_003343) - 3952580..3952798 (-) 219 WP_002814034.1 YdcH family protein -
  H7A87_RS16645 (XSP_003344) - 3953144..3954034 (-) 891 WP_115677892.1 hypothetical protein -
  H7A87_RS16650 (XSP_003345) - 3954031..3954414 (-) 384 WP_039525328.1 DUF4398 domain-containing protein -
  H7A87_RS16655 (XSP_003346) pilU 3954513..3955646 (+) 1134 WP_119128315.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41825.19 Da        Isoelectric Point: 6.1401

>NTDB_id=1132463 H7A87_RS16655 WP_119128315.1 3954513..3955646(+) (pilU) [Xanthomonas sp. CPBF 426 isolate]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSLRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAATPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1132463 H7A87_RS16655 WP_119128315.1 3954513..3955646(+) (pilU) [Xanthomonas sp. CPBF 426 isolate]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAGAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGAATGGTCAAGAAAATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCAGGACGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGTGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATTCCCAGCATCGA
AGAGCTGAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCATGCCTTCCAGAG
CGCGTTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAGACCATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTTCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCCGTGGTGTCGCTGCG
CCTGGTCAAGGGCGTGGACGGACGCCGCCGGCCGGCGACCGAGGTACTGCTCAACACGCCGATGATCCGCGATCTGCTGC
GCCGCGGCCAGGTGCACGAGGTTAAGCAGGCGATGGAAGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGTCCGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGGCTGGCGCTGAA
GTTCCGCCTGTCCGAGGGCGGCTCCGGCGAGCACGACCCGTATGCGGACTACGAAACGGCAGCGACACCGAAGATCAGCC
ACGGCTTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

48.889

95.491

0.467
  pilU Acinetobacter baylyi ADP1

49.153

93.899

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.979

100

0.446
  pilT Pseudomonas aeruginosa PAK

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Acinetobacter baumannii D1279779

40.828

89.655

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366
  pilT Acinetobacter nosocomialis M2

40.828

89.655

0.366
  pilT Acinetobacter baumannii strain A118

40.828

89.655

0.366
  pilT Acinetobacter baylyi ADP1

40.294

90.186

0.363

Multiple sequence alignment