Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   H1W80_RS08460 Genome accession   NZ_LR822040
Coordinates   1634211..1635152 (-) Length   313 a.a.
NCBI ID   WP_022096896.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_1125     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1629211..1640152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W80_RS08420 (STHERMO_1901) - 1629287..1630480 (-) 1194 WP_180473352.1 acetate kinase -
  H1W80_RS08425 (STHERMO_1902) comYH 1630536..1631492 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  H1W80_RS08430 (STHERMO_1903) comGG 1631537..1631854 (-) 318 WP_002952035.1 competence type IV pilus minor pilin ComGG -
  H1W80_RS08435 (STHERMO_1904) comYF 1631832..1632269 (-) 438 WP_082309153.1 competence type IV pilus minor pilin ComGF Machinery gene
  H1W80_RS08440 (STHERMO_1905) comGE 1632253..1632546 (-) 294 WP_180473353.1 competence type IV pilus minor pilin ComGE -
  H1W80_RS08445 (STHERMO_1906) comYD 1632518..1632946 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  H1W80_RS08450 (STHERMO_1907) comYC 1632906..1633232 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  H1W80_RS08455 (STHERMO_1908) comYB 1633229..1634329 (-) 1101 WP_180473354.1 competence type IV pilus assembly protein ComGB Machinery gene
  H1W80_RS08460 (STHERMO_1909) comGA/cglA/cilD 1634211..1635152 (-) 942 WP_022096896.1 competence type IV pilus ATPase ComGA Machinery gene
  H1W80_RS08465 (STHERMO_1910) - 1635233..1635595 (-) 363 WP_014608746.1 DUF1033 family protein -
  H1W80_RS08470 (STHERMO_1911) rpoC 1635754..1639392 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35409.56 Da        Isoelectric Point: 6.3927

>NTDB_id=1132027 H1W80_RS08460 WP_022096896.1 1634211..1635152(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1125]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVAGMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1132027 H1W80_RS08460 WP_022096896.1 1634211..1635152(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1125]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCTATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTCTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGATCTTGGTGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.595

99.361

0.652
  comYA Streptococcus mutans UA159

64.856

100

0.649
  comYA Streptococcus mutans UA140

64.856

100

0.649
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.309

99.361

0.639
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.309

99.361

0.639
  comYA Streptococcus gordonii str. Challis substr. CH1

63.259

100

0.633
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.538

99.681

0.613
  comGA Lactococcus lactis subsp. cremoris KW2

51.125

99.361

0.508
  comGA Latilactobacillus sakei subsp. sakei 23K

38.816

97.125

0.377

Multiple sequence alignment