Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   H1W89_RS08725 Genome accession   NZ_LR822029
Coordinates   1674237..1675178 (-) Length   313 a.a.
NCBI ID   WP_014608745.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_1035     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1669237..1680178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1W89_RS08685 (STHERMO_1935) - 1669313..1670506 (-) 1194 WP_014608740.1 acetate kinase -
  H1W89_RS08690 (STHERMO_1936) comYH 1670562..1671518 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  H1W89_RS08695 (STHERMO_1937) comGG 1671563..1671880 (-) 318 WP_180474625.1 competence type IV pilus minor pilin ComGG -
  H1W89_RS08700 (STHERMO_1938) comYF 1671858..1672295 (-) 438 WP_014608742.1 competence type IV pilus minor pilin ComGF Machinery gene
  H1W89_RS08705 (STHERMO_1939) comGE 1672279..1672572 (-) 294 WP_011226625.1 competence type IV pilus minor pilin ComGE -
  H1W89_RS08710 (STHERMO_1940) comYD 1672544..1672972 (-) 429 WP_011226626.1 competence type IV pilus minor pilin ComGD Machinery gene
  H1W89_RS08715 (STHERMO_1941) comYC 1672932..1673258 (-) 327 WP_002946126.1 competence type IV pilus major pilin ComGC Machinery gene
  H1W89_RS08720 (STHERMO_1942) comYB 1673255..1674355 (-) 1101 WP_116920398.1 competence type IV pilus assembly protein ComGB Machinery gene
  H1W89_RS08725 (STHERMO_1943) comGA/cglA/cilD 1674237..1675178 (-) 942 WP_014608745.1 competence type IV pilus ATPase ComGA Machinery gene
  H1W89_RS08730 (STHERMO_1944) - 1675259..1675621 (-) 363 WP_014608746.1 DUF1033 family protein -
  H1W89_RS08735 (STHERMO_1945) rpoC 1675780..1679418 (-) 3639 WP_011226631.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35508.70 Da        Isoelectric Point: 6.6091

>NTDB_id=1131432 H1W89_RS08725 WP_014608745.1 1674237..1675178(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1035]
MVTEFAKEMIKNADSCGAQDIYVIPRQDNYELYMRVGQERRLIDVYRPDFMASLIGHFKFVARMMVGEKRRSQLGSCDYD
CGDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIKDALDYRGLYLFAGPVGSGKTTLMHELVQERFKGQ
QVISIEDPVEIKQDNVLQLQVNQAIDMTYDSLIKLSLRHRPDVLIIGEIRDKETARAVIRASLTGVTVLSTIHAKSIAGV
YERLLDLGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNQQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1131432 H1W89_RS08725 WP_014608745.1 1674237..1675178(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_1035]
ATGGTAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATGTCATTCCACGTCA
GGATAATTATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTGGTCACTTTAAATTTGTGGCGAGAATGATGGTGGGTGAGAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGGTGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTAAGGATGCCTTGGATTATAGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACCACACTTATGCATGAGTTGGTTCAGGAGCGTTTTAAGGGGCAG
CAGGTAATTTCTATTGAGGATCCTGTTGAAATCAAACAGGATAATGTCTTGCAACTCCAGGTTAATCAGGCGATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATGTTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATTAGAGCTAGTCTAACAGGTGTAACTGTTCTCTCAACTATTCATGCTAAGAGTATTGCTGGCGTT
TACGAACGTCTCTTGGACCTTGGTGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCAGCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.273

99.361

0.649
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus pneumoniae D39

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae R6

63.987

99.361

0.636
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

63.987

99.361

0.636
  comYA Streptococcus gordonii str. Challis substr. CH1

62.939

100

0.629
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.218

99.681

0.61
  comGA Lactococcus lactis subsp. cremoris KW2

50.804

99.361

0.505
  comGA Latilactobacillus sakei subsp. sakei 23K

38.487

97.125

0.374

Multiple sequence alignment