Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   H0511_RS08730 Genome accession   NZ_LR822017
Coordinates   1708813..1709754 (-) Length   313 a.a.
NCBI ID   WP_179974044.1    Uniprot ID   -
Organism   Streptococcus thermophilus isolate STH_CIRM_336     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1703813..1714754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H0511_RS08690 (STHERMO_1966) - 1703889..1705082 (-) 1194 WP_014608740.1 acetate kinase -
  H0511_RS08695 (STHERMO_1967) comYH 1705138..1706094 (-) 957 WP_011226622.1 class I SAM-dependent methyltransferase Machinery gene
  H0511_RS08700 (STHERMO_1968) comGG 1706139..1706456 (-) 318 WP_011681685.1 competence type IV pilus minor pilin ComGG -
  H0511_RS08705 (STHERMO_1969) comYF 1706434..1706871 (-) 438 WP_179974042.1 competence type IV pilus minor pilin ComGF Machinery gene
  H0511_RS08710 (STHERMO_1970) comGE 1706855..1707148 (-) 294 WP_179972344.1 competence type IV pilus minor pilin ComGE -
  H0511_RS08715 (STHERMO_1971) comGD 1707120..1707548 (-) 429 WP_269473123.1 competence type IV pilus minor pilin ComGD -
  H0511_RS08720 (STHERMO_1972) comYC 1707508..1707834 (-) 327 WP_179972346.1 competence type IV pilus major pilin ComGC Machinery gene
  H0511_RS08725 (STHERMO_1973) comYB 1707831..1708931 (-) 1101 WP_179974043.1 competence type IV pilus assembly protein ComGB Machinery gene
  H0511_RS08730 (STHERMO_1974) comGA/cglA/cilD 1708813..1709754 (-) 942 WP_179974044.1 competence type IV pilus ATPase ComGA Machinery gene
  H0511_RS08735 (STHERMO_1975) - 1709835..1710197 (-) 363 WP_179972348.1 DUF1033 family protein -
  H0511_RS08740 (STHERMO_1976) rpoC 1710356..1713994 (-) 3639 WP_179974045.1 DNA-directed RNA polymerase subunit beta' -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35611.85 Da        Isoelectric Point: 6.1042

>NTDB_id=1130995 H0511_RS08730 WP_179974044.1 1708813..1709754(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_336]
MITEFAKEMIKNADSCGAQDIYFIPRQDKYELYMRVGQERRLIDVYRPDFMASLISHFKFVAGMMVGEKRRSQLGSCDYD
CDDGHLVSLRLSTVGDYRGLESLVIRILHSERRELVYWNQGIQPIMDALDYRGLYLFAGPVGSGKTTLMHELVQECFKGQ
QVISIEDPVEIKQDDVLQLQVNKAIDMTYDSLIKLSLRHRPDILIIGEIRDKETARAVIRASLTGVTVLSTIHAKSITGV
YERLLDFGVDKSELDNALQGIAYMRLIKGGGVIDFARENFQNHSSTNWNHQLEGLVKQGCLTERDIQGEKIKD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1130995 H0511_RS08730 WP_179974044.1 1708813..1709754(-) (comGA/cglA/cilD) [Streptococcus thermophilus isolate STH_CIRM_336]
ATGATAACAGAATTTGCTAAGGAAATGATCAAAAATGCTGATAGTTGTGGGGCTCAAGACATCTATTTCATTCCACGTCA
GGATAAATATGAGCTCTATATGCGAGTCGGGCAAGAGAGGAGATTGATTGATGTATATCGGCCTGATTTCATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCGGGAATGATGGTGGGTGAAAAGCGTAGGAGTCAACTGGGTTCATGTGACTATGAT
TGTGATGACGGTCACTTGGTTTCTCTGCGTTTATCAACTGTTGGAGACTATCGAGGTTTGGAGAGCTTGGTTATCCGTAT
CCTACATTCCGAACGTCGAGAATTAGTGTATTGGAATCAAGGAATCCAGCCTATTATGGATGCCTTGGATTATCGAGGAC
TGTATCTCTTTGCGGGTCCCGTAGGTTCTGGGAAGACTACACTTATGCATGAGTTGGTTCAGGAGTGTTTTAAGGGGCAG
CAGGTAATTTCGATTGAGGATCCTGTTGAAATCAAACAGGATGATGTCTTGCAACTCCAGGTTAATAAGGCAATTGATAT
GACCTATGATAGTTTGATTAAGCTATCTCTACGTCACCGTCCAGATATTTTGATTATTGGGGAGATTCGGGATAAGGAGA
CTGCCCGGGCAGTTATCAGAGCTAGTCTAACAGGTGTAACTGTTCTTTCAACTATTCATGCTAAGAGTATTACTGGCGTT
TACGAACGTCTCTTGGACTTTGGCGTAGATAAGTCTGAGTTGGATAATGCTCTACAAGGCATTGCCTACATGCGTTTGAT
CAAGGGAGGAGGTGTGATTGATTTTGCCAGAGAAAATTTCCAAAACCATTCGTCGACCAACTGGAATCATCAGTTGGAAG
GTTTGGTTAAACAAGGATGTCTCACTGAGAGGGATATCCAAGGGGAAAAAATTAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

65.273

99.361

0.649
  comYA Streptococcus mutans UA159

64.537

100

0.645
  comYA Streptococcus mutans UA140

64.537

100

0.645
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae R6

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

64.63

99.361

0.642
  comGA/cglA/cilD Streptococcus pneumoniae D39

64.63

99.361

0.642
  comYA Streptococcus gordonii str. Challis substr. CH1

63.578

100

0.636
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.577

99.681

0.604
  comGA Lactococcus lactis subsp. cremoris KW2

50.804

99.361

0.505
  comGA Latilactobacillus sakei subsp. sakei 23K

38.591

95.208

0.367

Multiple sequence alignment