Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   E0F31_RS10075 Genome accession   NZ_LR216064
Coordinates   1865207..1866148 (-) Length   313 a.a.
NCBI ID   WP_000249555.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1860207..1871148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F31_RS10035 (SAMEA3714520_01891) comYH 1860700..1861653 (-) 954 WP_000345083.1 class I SAM-dependent methyltransferase Machinery gene
  E0F31_RS10040 (SAMEA3714520_01892) - 1861714..1862301 (-) 588 WP_000679775.1 class I SAM-dependent methyltransferase -
  E0F31_RS10045 (SAMEA3714520_01893) comGG/cglG 1862438..1862851 (-) 414 WP_000265617.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F31_RS10050 (SAMEA3714520_01894) comGF/cglF 1862829..1863290 (-) 462 WP_000250546.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F31_RS10055 (SAMEA3714520_01895) comGE/cglE 1863253..1863555 (-) 303 WP_257641679.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F31_RS10060 (SAMEA3714520_01896) comGD/cglD 1863518..1863922 (-) 405 WP_000588016.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F31_RS10065 (SAMEA3714520_01897) comGC/cglC 1863915..1864241 (-) 327 WP_000738632.1 comG operon protein ComGC Machinery gene
  E0F31_RS10070 (SAMEA3714520_01898) comGB/cglB 1864243..1865259 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F31_RS10075 (SAMEA3714520_01899) comGA/cglA/cilD 1865207..1866148 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F31_RS10080 (SAMEA3714520_01900) - 1866224..1866589 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F31_RS10085 (SAMEA3714520_01901) - 1866740..1867798 (-) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F31_RS10090 (SAMEA3714520_01902) nagA 1867961..1869112 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  E0F31_RS10095 (SAMEA3714520_01903) - 1869265..1871082 (-) 1818 WP_001220845.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35561.49 Da        Isoelectric Point: 6.0083

>NTDB_id=1126301 E0F31_RS10075 WP_000249555.1 1865207..1866148(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1126301 E0F31_RS10075 WP_000249555.1 1865207..1866148(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACTATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.722

100

0.987
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.722

100

0.987
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

97.125

100

0.971
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment