Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   E0F31_RS10050 Genome accession   NZ_LR216064
Coordinates   1862829..1863290 (-) Length   153 a.a.
NCBI ID   WP_000250546.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1857829..1868290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F31_RS10020 (SAMEA3714520_01888) - 1858140..1858964 (-) 825 WP_000727906.1 membrane protein insertase YidC -
  E0F31_RS10025 (SAMEA3714520_01889) rnpA 1858939..1859310 (-) 372 WP_000739248.1 ribonuclease P protein component -
  E0F31_RS12135 - 1859327..1859458 (-) 132 WP_000768902.1 hypothetical protein -
  E0F31_RS10030 (SAMEA3714520_01890) - 1859459..1860649 (-) 1191 WP_050085692.1 acetate kinase -
  E0F31_RS10035 (SAMEA3714520_01891) comYH 1860700..1861653 (-) 954 WP_000345083.1 class I SAM-dependent methyltransferase Machinery gene
  E0F31_RS10040 (SAMEA3714520_01892) - 1861714..1862301 (-) 588 WP_000679775.1 class I SAM-dependent methyltransferase -
  E0F31_RS10045 (SAMEA3714520_01893) comGG/cglG 1862438..1862851 (-) 414 WP_000265617.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F31_RS10050 (SAMEA3714520_01894) comGF/cglF 1862829..1863290 (-) 462 WP_000250546.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F31_RS10055 (SAMEA3714520_01895) comGE/cglE 1863253..1863555 (-) 303 WP_257641679.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F31_RS10060 (SAMEA3714520_01896) comGD/cglD 1863518..1863922 (-) 405 WP_000588016.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F31_RS10065 (SAMEA3714520_01897) comGC/cglC 1863915..1864241 (-) 327 WP_000738632.1 comG operon protein ComGC Machinery gene
  E0F31_RS10070 (SAMEA3714520_01898) comGB/cglB 1864243..1865259 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F31_RS10075 (SAMEA3714520_01899) comGA/cglA/cilD 1865207..1866148 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F31_RS10080 (SAMEA3714520_01900) - 1866224..1866589 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F31_RS10085 (SAMEA3714520_01901) - 1866740..1867798 (-) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17839.42 Da        Isoelectric Point: 8.8053

>NTDB_id=1126296 E0F31_RS10050 WP_000250546.1 1862829..1863290(-) (comGF/cglF) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVRFRFQFQKGLEREFIYRVEKEKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1126296 E0F31_RS10050 WP_000250546.1 1862829..1863290(-) (comGF/cglF) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
TGTCATCAGTGGGGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGAGGTTATCAGCCTATGGTTTATGGGCTCAAATCCGCACAGATTACAGAGGACAATCAACTGGTGCGCTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

95.425

100

0.954
  comGF/cglF Streptococcus pneumoniae D39

95.425

100

0.954
  comGF/cglF Streptococcus pneumoniae R6

95.425

100

0.954
  comGF/cglF Streptococcus pneumoniae TIGR4

95.425

100

0.954
  comGF/cglF Streptococcus mitis SK321

94.118

100

0.941
  comGF/cglF Streptococcus mitis NCTC 12261

92.81

100

0.928
  comGF Lactococcus lactis subsp. cremoris KW2

40

91.503

0.366

Multiple sequence alignment