Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   E0F31_RS10070 Genome accession   NZ_LR216064
Coordinates   1864243..1865259 (-) Length   338 a.a.
NCBI ID   WP_077141332.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1859243..1870259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F31_RS12135 - 1859327..1859458 (-) 132 WP_000768902.1 hypothetical protein -
  E0F31_RS10030 (SAMEA3714520_01890) - 1859459..1860649 (-) 1191 WP_050085692.1 acetate kinase -
  E0F31_RS10035 (SAMEA3714520_01891) comYH 1860700..1861653 (-) 954 WP_000345083.1 class I SAM-dependent methyltransferase Machinery gene
  E0F31_RS10040 (SAMEA3714520_01892) - 1861714..1862301 (-) 588 WP_000679775.1 class I SAM-dependent methyltransferase -
  E0F31_RS10045 (SAMEA3714520_01893) comGG/cglG 1862438..1862851 (-) 414 WP_000265617.1 competence type IV pilus minor pilin ComGG Machinery gene
  E0F31_RS10050 (SAMEA3714520_01894) comGF/cglF 1862829..1863290 (-) 462 WP_000250546.1 competence type IV pilus minor pilin ComGF Machinery gene
  E0F31_RS10055 (SAMEA3714520_01895) comGE/cglE 1863253..1863555 (-) 303 WP_257641679.1 competence type IV pilus minor pilin ComGE Machinery gene
  E0F31_RS10060 (SAMEA3714520_01896) comGD/cglD 1863518..1863922 (-) 405 WP_000588016.1 competence type IV pilus minor pilin ComGD Machinery gene
  E0F31_RS10065 (SAMEA3714520_01897) comGC/cglC 1863915..1864241 (-) 327 WP_000738632.1 comG operon protein ComGC Machinery gene
  E0F31_RS10070 (SAMEA3714520_01898) comGB/cglB 1864243..1865259 (-) 1017 WP_077141332.1 competence type IV pilus assembly protein ComGB Machinery gene
  E0F31_RS10075 (SAMEA3714520_01899) comGA/cglA/cilD 1865207..1866148 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  E0F31_RS10080 (SAMEA3714520_01900) - 1866224..1866589 (-) 366 WP_000286415.1 DUF1033 family protein -
  E0F31_RS10085 (SAMEA3714520_01901) - 1866740..1867798 (-) 1059 WP_000649470.1 zinc-dependent alcohol dehydrogenase family protein -
  E0F31_RS10090 (SAMEA3714520_01902) nagA 1867961..1869112 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38466.48 Da        Isoelectric Point: 9.6162

>NTDB_id=1126300 E0F31_RS10070 WP_077141332.1 1864243..1865259(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1126300 E0F31_RS10070 WP_077141332.1 1864243..1865259(-) (comGB/cglB) [Streptococcus pneumoniae strain GPSC13 substr. ST473 isolate 569492b0-41bd-11e5-998e-3c4a9275d6c6]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

95.266

100

0.953
  comGB/cglB Streptococcus mitis NCTC 12261

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment